Construct: sgRNA BRDN0001147473
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGAGCAAGGCATCAGACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ALPK2 (115701)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77523
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
58536399 |
- |
ALPK2 |
NNNAGCAAGGCATCAGACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
2279101 |
+ |
ABCA3 |
NNNAGCAAGGCATCAAACTG |
NGG |
2 |
0.6667 |
Tier I |
| 3 |
NC_000004.12 |
4 |
85866777 |
+ |
ARHGAP24 |
NNNACCAAGGCATCAGATGG |
NGG |
2 |
0.5051 |
Tier II |
| 4 |
NC_000004.12 |
4 |
5932439 |
+ |
C4orf50 |
NNNAGGAAGGCATCAGACTG |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000006.12 |
6 |
33773238 |
+ |
LEMD2 |
NNNAGCTAAGCATCAGACGG |
NGG |
2 |
0.2812 |
Tier II |
| 6 |
NC_000021.9 |
21 |
46438485 |
- |
PCNT |
NNNAGCAAGGCTTCAGACAG |
NGG |
2 |
0.2381 |
Tier II |
| 7 |
NC_000002.12 |
2 |
1874626 |
- |
MYT1L |
NNNAGCAGGGCCTCAGACGG |
NGG |
2 |
0.193 |
Tier II |
| 8 |
NC_000007.14 |
7 |
93247571 |
- |
VPS50 |
NNNATGAAGGCATCAGACGG |
NGG |
2 |
0.15 |
Tier II |
| 9 |
NC_000005.10 |
5 |
52791505 |
- |
ITGA1 |
NNNAGCAAGACATCAGAGGG |
NGG |
2 |
0.1244 |
Tier II |
| 10 |
NC_000005.10 |
5 |
52791505 |
- |
PELO |
NNNAGCAAGACATCAGAGGG |
NGG |
2 |
0.1244 |
Tier II |
| 11 |
NC_000015.10 |
15 |
27375450 |
+ |
GABRG3 |
NNNAGCAAAGCATCAGACGG |
NGA |
2 |
0.0446 |
Tier II |
| 12 |
NC_000008.11 |
8 |
138234061 |
+ |
FAM135B |
NNNAGCAAGGCATCAGAAGG |
NTG |
2 |
0.021 |
Tier II |
| 13 |
NC_000002.12 |
2 |
146915434 |
+ |
LOC107985824 |
NNNAGCAAGGCATCAGAAGG |
NGG |
1 |
0.5385 |
Tier III |
| 14 |
NC_000005.10 |
5 |
52791505 |
- |
PELO-AS1 |
NNNAGCAAGACATCAGAGGG |
NGG |
2 |
0.1244 |
Tier III |
| 15 |
NC_000006.12 |
6 |
146973323 |
- |
STXBP5-AS1 |
NNNAGAAAGGCATCAGAGGG |
NGG |
2 |
0.1238 |
Tier III |
| 16 |
NC_000015.10 |
15 |
77985063 |
- |
ADAMTS7P3 |
NNNAGCATGGCATCAGACGG |
NTG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
120317931 |
- |
Abca14 |
NNNAGCAAGGCATCAAACTG |
NGG |
2 |
0.6667 |
Tier I |
| 2 |
NC_000068.7 |
2 |
73332970 |
- |
Scrn3 |
NNNAGCAAGGCACCAGACAG |
NGG |
2 |
0.5639 |
Tier II |
| 3 |
NC_000079.6 |
13 |
41200618 |
- |
Elovl2 |
NNNAGCAAGGAAACAGACGG |
NGG |
2 |
0.5192 |
Tier II |
| 4 |
NC_000071.6 |
5 |
48214075 |
- |
Slit2 |
NNNAGCAATTCATCAGACGG |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000077.6 |
11 |
67066466 |
+ |
Sco1 |
NNNAGGAAGGCATCAGATGG |
NGG |
2 |
0.3214 |
Tier II |
| 6 |
NC_000076.6 |
10 |
29333498 |
- |
Echdc1 |
NNNAGGAAGGCATCAGAAGG |
NGG |
2 |
0.2692 |
Tier II |
| 7 |
NC_000082.6 |
16 |
90964083 |
+ |
Synj1 |
NNNAGCAAGACAGCAGACGG |
NGG |
2 |
0.2435 |
Tier II |
| 8 |
NC_000070.6 |
4 |
120438141 |
- |
Scmh1 |
NNNAGGAAGCCATCAGACGG |
NGG |
2 |
0.2 |
Tier II |
| 9 |
NC_000078.6 |
12 |
86262977 |
- |
Gpatch2l |
NNNAGCAAGGCAGCAGATGG |
NGG |
2 |
0.1677 |
Tier II |
| 10 |
NC_000081.6 |
15 |
98913633 |
+ |
Lmbr1l |
NNNAGCAAGACATCAGAGGG |
NGG |
2 |
0.1244 |
Tier II |
| 11 |
NC_000079.6 |
13 |
57525201 |
- |
Spock1 |
NNNAGCAAAGCATCAGAGGG |
NGG |
2 |
0.0857 |
Tier II |
| 12 |
NC_000072.6 |
6 |
137772380 |
- |
Dera |
NNNAACAAGGCATCACACGG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000077.6 |
11 |
86081690 |
- |
Brip1 |
NNNAGCAAGGCATCATACAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000075.6 |
9 |
43616458 |
+ |
Gm29909 |
NNNAGCAAGGCTTCAGATGG |
NGG |
2 |
0.2143 |
Tier III |
| 15 |
NC_000070.6 |
4 |
150058676 |
- |
Gm13067 |
NNNAACAAGGCATCAGAGGG |
NGG |
2 |
0.1156 |
Tier III |
Other clones with same target sequence:
(none)
