Construct: sgRNA BRDN0001147535

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGATACTTCATCCCACCAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK4 (814)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77227

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 111478473 - CAMK4 NNNTACTTCATCCCACCAGG NGG 0 1.0 Tier I
2 NC_000016.10 16 55698835 + SLC6A2 NNNTAATTCATCCTACCAGG NGG 2 0.681 Tier II
3 NC_000012.12 12 121668892 - MORN3 NNNTACTCCATCCCACAAGG NGG 2 0.4667 Tier II
4 NC_000020.11 20 58693534 + NPEPL1 NNNGGCTTCATCCCACCAGG NGG 2 0.45 Tier II
5 NC_000011.10 11 132803517 - OPCML NNNTACCTCATTCCACCAGG NGG 2 0.3702 Tier II
6 NC_000018.10 18 33149091 - CCDC178 NNNTTCTTCATCCCAACAGG NGG 2 0.3636 Tier II
7 NC_000005.10 5 119364070 - TNFAIP8 NNNGACTTCATCCCACCTGG NGG 2 0.3125 Tier II
8 NC_000004.12 4 158586705 - RXFP1 NNNTACTTCCTCCCACCAGA NGG 2 0.3125 Tier II
9 NC_000008.11 8 104143468 - RIMS2 NNNTACTTCATCACACCAGT NGG 2 0.2692 Tier II
10 NC_000009.12 9 127545807 - NIBAN2 NNNGACTTCCTCCCACCAGG NGG 2 0.2083 Tier II
11 NC_000023.11 X 47880106 + ZNF81 NNNTAATTCATCCCTCCAGG NGG 2 0.1857 Tier II
12 NC_000004.12 4 90655293 - CCSER1 NNNTACTTCATCAAACCAGG NGG 2 0.1346 Tier II
13 NC_000017.11 17 79231309 - RBFOX3 NNNTCCTTCATCCCTCCAGG NGG 2 0.1 Tier II
14 NC_000008.11 8 41931724 - KAT6A NNNTACTTCATTCCAGCAGG NGG 2 0.0828 Tier II
15 NC_000008.11 8 31911030 + NRG1 NNNTACTTCATTGCACCAGG NGG 2 0.0734 Tier II
16 NC_000017.11 17 70128433 - KCNJ16 NNNTACTTCATCCCACGAGG NGG 1 0.0588 Tier II
17 NC_000003.12 3 52470444 + NISCH NNNTCCTTCATCCCACCAGG NTG 2 0.0195 Tier II
18 NC_000005.10 5 1945151 - LOC124901165 NNNTACTTCATCCCACCGGG NTG 2 0.0156 Tier II
19 NC_000001.11 1 53730937 + GLIS1 NNNTACTTCATCCCACCAAG NGT 2 0.0115 Tier II
20 NC_000012.12 12 121668892 - LOC105370034 NNNTACTCCATCCCACAAGG NGG 2 0.4667 Tier III
21 NC_000020.11 20 58693534 + STX16-NPEPL1 NNNGGCTTCATCCCACCAGG NGG 2 0.45 Tier III
22 NC_000017.11 17 70128433 - LOC124904052 NNNTACTTCATCCCACGAGG NGG 1 0.0588 Tier III
23 NC_000021.9 21 38336413 - LINC01423 NNNTACTGCATCCCACCAGG NGA 2 0.0509 Tier III
24 NC_000005.10 5 1945151 - LOC105374618 NNNTACTTCATCCCACCGGG NTG 2 0.0156 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 33179590 - Camk4 NNNCACTTCATCCCACCAGG NGG 1 0.6471 Tier I
2 NC_000077.6 11 69050300 + Aurkb NNNTCCCTCATCCCACCAGG NGG 2 0.3438 Tier I
3 NC_000072.6 6 40465906 - Wee2 NNNTCCTTCCTCCCACCAGG NGG 2 0.1667 Tier I
4 NC_000073.6 7 84035999 - Cemip NNNTAATTCATCCTACCAGG NGG 2 0.681 Tier II
5 NC_000081.6 15 58430411 - Fam91a1 NNNTATTTCATCCCACCTGG NGG 2 0.4643 Tier II
6 NC_000078.6 12 83692986 - Psen1 NNNCACTTCATCCCACCAAG NGG 2 0.4622 Tier II
7 NC_000072.6 6 65049298 + Smarcad1 NNNTACTTCAGCTCACCAGG NGG 2 0.28 Tier II
8 NC_000078.6 12 91292848 - Cep128 NNNGACTTCAGCCCACCAGG NGG 2 0.25 Tier II
9 NC_000075.6 9 39142221 - Olfr27 NNNTACTTCATCCAACCAGG NAG 2 0.0907 Tier II
10 NC_000072.6 6 35316024 - Fam180a NNNTCCTTCATCCCACCAGG NGA 2 0.0347 Tier II
11 NC_000070.6 4 58524784 - Lpar1 NNNTACTTCCTCCCACCAGG NTG 2 0.013 Tier II
12 NC_000067.6 1 66379448 - Map2 NNNTACTTCATTCCACCAGG NGC 2 0.012 Tier II
13 NC_000073.6 7 71684874 + Gm44689 NNNTAATTCATCCCACCATG NGG 2 0.619 Tier III
14 NC_000084.6 18 40795913 + Gm41710 NNNTACTTGATCCCACCATG NGG 2 0.4127 Tier III
15 NC_000085.6 19 3733316 + Gm36787 NNNTACTTCATTCTACCAGG NGG 2 0.3949 Tier III
16 NC_000078.6 12 93440809 - 4930559C10Rik NNNTTCTTCATCCCCCCAGG NGG 2 0.0992 Tier III
17 NC_000072.6 6 35316024 - Gm38788 NNNTCCTTCATCCCACCAGG NGA 2 0.0347 Tier III
Download CSV

Other clones with same target sequence:

(none)