Construct: sgRNA BRDN0001147549

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCCTGGTCGGCCAGTCACGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAST3 (23031)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76104

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 18128890 + MAST3 NNNTGGTCGGCCAGTCACGG NGG 0 1.0 Tier I
2 NC_000017.11 17 7743393 + DNAH2 NNNTGATCGGCCAGGCACGG NGG 2 0.05 Tier I
3 NC_000012.12 12 82893099 + TMTC2 NNNTGGTGGGCCAGTCACGG NGG 1 0.6429 Tier II
4 NC_000001.11 1 15483098 - CELA2B NNNGGGTCGGCCAGTCACGG NGG 1 0.625 Tier II
5 NC_000003.12 3 48792431 - PRKAR2A NNNTAGTGGGCCAGTCACGG NGG 2 0.5571 Tier II
6 NC_000001.11 1 163222492 - RGS5 NNNTGGTCCACCAGTCACGG NGG 2 0.5026 Tier II
7 NC_000010.11 10 109905499 - XPNPEP1 NNNTGCTGGGCCAGTCACGG NGG 2 0.4383 Tier II
8 NC_000001.11 1 51922988 - RAB3B NNNTGGTGGGCCAGTCACTG NGG 2 0.4286 Tier II
9 NC_000001.11 1 13488561 - LRRC38 NNNTGTTCAGCCAGTCACGG NGG 2 0.4286 Tier II
10 NC_000014.9 14 69193350 - EXD2 NNNTGGTGGGCCAGACACGG NGG 2 0.3722 Tier II
11 NC_000018.10 18 79455350 - NFATC1 NNNTGGCCTGCCAGTCACGG NGG 2 0.3667 Tier II
12 NC_000007.14 7 140001683 - TBXAS1 NNNTTATCGGCCAGTCACGG NGG 2 0.3 Tier II
13 NC_000004.12 4 109794519 + CFI NNNTTGTTGGCCAGTCACGG NGG 2 0.2625 Tier II
14 NC_000001.11 1 45631554 - GPBP1L1 NNNTTGTAGGCCAGTCACGG NGG 2 0.195 Tier II
15 NC_000009.12 9 72219132 + GDA NNNTGGTCGGCCGGTCGCGG NGG 2 0.1151 Tier II
16 NC_000001.11 1 218392079 + TGFB2 NNNTGATCGGCCAGGCACGG NGG 2 0.05 Tier II
17 NC_000007.14 7 43598198 + STK17A NNNTGGTCGGCCAGGCACGG NGG 1 0.05 Tier II
18 NC_000015.10 15 52779379 - ONECUT1 NNNTGGTCGGCCAGGCACGG NGG 1 0.05 Tier II
19 NC_000002.12 2 218667507 - RNF25 NNNTGGTTGGCCAGGCACGG NGG 2 0.0438 Tier II
20 NC_000003.12 3 132363666 + ACP3 NNNTGGTTGGCCAGGCACGG NGG 2 0.0438 Tier II
21 NC_000004.12 4 165303855 - KLHL2 NNNTGGTTGGCCAGGCACGG NGG 2 0.0438 Tier II
22 NC_000007.14 7 5367719 - TNRC18 NNNTGGTTGGCCAGGCACGG NGG 2 0.0438 Tier II
23 NC_000010.11 10 101014150 - PDZD7 NNNTGGTTGGCCAGGCACGG NGG 2 0.0438 Tier II
24 NC_000011.10 11 89494046 - NOX4 NNNTGGTTGGCCAGGCACGG NGG 2 0.0438 Tier II
25 NC_000011.10 11 132081460 + NTM NNNTGGTTGGCCAGGCACGG NGG 2 0.0438 Tier II
26 NC_000017.11 17 66652250 - PRKCA NNNTGGTTGGCCAGGCACGG NGG 2 0.0438 Tier II
27 NC_000001.11 1 225984538 + SDE2 NNNTCGTCGGCCAGGCACGG NGG 2 0.0393 Tier II
28 NC_000006.12 6 31798363 - LSM2 NNNTCGTCGGCCAGGCACGG NGG 2 0.0393 Tier II
29 NC_000012.12 12 94417516 + CEP83 NNNTGGTCGGCCAGGCACAG NGG 2 0.0357 Tier II
30 NC_000003.12 3 12743195 + TMEM40 NNNTGCTCGGCCAGGCACGG NGG 2 0.0341 Tier II
31 NC_000006.12 6 169464848 + WDR27 NNNTGCTCGGCCAGGCACGG NGG 2 0.0341 Tier II
32 NC_000008.11 8 117979082 + EXT1 NNNTGCTCGGCCAGGCACGG NGG 2 0.0341 Tier II
33 NC_000016.10 16 71178147 + HYDIN NNNTGCTCGGCCAGGCACGG NGG 2 0.0341 Tier II
34 NC_000003.12 3 113935035 - GRAMD1C NNNTGTTCGGCCAGGCACGG NGG 2 0.0333 Tier II
35 NC_000012.12 12 12742494 + APOLD1 NNNTGGTCGGCCGGGCACGG NGG 2 0.0326 Tier II
36 NC_000001.11 1 26742698 + ARID1A NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
37 NC_000001.11 1 51953531 + RAB3B NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
38 NC_000001.11 1 215136024 - KCNK2 NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
39 NC_000003.12 3 138278235 + ARMC8 NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
40 NC_000003.12 3 138278235 + NME9 NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
41 NC_000005.10 5 119109092 - DMXL1 NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
42 NC_000006.12 6 108046311 - OSTM1 NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
43 NC_000007.14 7 149078412 + ZNF786 NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
44 NC_000008.11 8 42885950 + RNF170 NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
45 NC_000008.11 8 123257210 - ZHX1 NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
46 NC_000008.11 8 123257210 - ZHX1-C8orf76 NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier II
47 NC_000006.12 6 123398296 - TRDN NNNCGGTCGGCCAGGCACGG NGG 2 0.0324 Tier II
48 NC_000007.14 7 1836311 - MAD1L1 NNNTGGTCAGCCAGGCACGG NGG 2 0.0321 Tier II
49 NC_000010.11 10 27869826 - ODAD2 NNNTGGTCGGCCAGGCATGG NGG 2 0.0321 Tier II
50 NC_000002.12 2 70059182 - ASPRV1 NNNTGGTGGGCCAGGCACGG NGG 2 0.0321 Tier II
51 NC_000006.12 6 24484206 - GPLD1 NNNTGGTGGGCCAGGCACGG NGG 2 0.0321 Tier II
52 NC_000008.11 8 43143837 - HGSNAT NNNTGGTGGGCCAGGCACGG NGG 2 0.0321 Tier II
53 NC_000010.11 10 60357000 - ANK3 NNNTGGTGGGCCAGGCACGG NGG 2 0.0321 Tier II
54 NC_000018.10 18 5436699 - EPB41L3 NNNTGGTGGGCCAGGCACGG NGG 2 0.0321 Tier II
55 NC_000001.11 1 26335525 + CRYBG2 NNNGGGTCGGCCAGGCACGG NGG 2 0.0313 Tier II
56 NC_000003.12 3 65516468 - MAGI1 NNNGGGTCGGCCAGGCACGG NGG 2 0.0313 Tier II
57 NC_000014.9 14 73069725 - RBM25 NNNGGGTCGGCCAGGCACGG NGG 2 0.0313 Tier II
58 NC_000019.10 19 51364916 + ETFB NNNGGGTCGGCCAGGCACGG NGG 2 0.0313 Tier II
59 NC_000006.12 6 30333652 - TRIM39 NNNTGGGCGGCCAGGCACGG NGG 2 0.0294 Tier II
60 NC_000006.12 6 30333652 - TRIM39-RPP21 NNNTGGGCGGCCAGGCACGG NGG 2 0.0294 Tier II
61 NC_000016.10 16 15755660 + MYH11 NNNTGGGCGGCCAGGCACGG NGG 2 0.0294 Tier II
62 NC_000001.11 1 11971659 - PLOD1 NNNAGGTCGGCCAGGCACGG NGG 2 0.0238 Tier II
63 NC_000006.12 6 158017706 - SYNJ2 NNNAGGTCGGCCAGGCACGG NGG 2 0.0238 Tier II
64 NC_000009.12 9 100468609 - MSANTD3-TMEFF1 NNNAGGTCGGCCAGGCACGG NGG 2 0.0238 Tier II
65 NC_000002.12 2 199429637 + SATB2 NNNTTGTCGGCCAGGCACGG NGG 2 0.015 Tier II
66 NC_000005.10 5 22389939 - CDH12 NNNTTGTCGGCCAGGCACGG NGG 2 0.015 Tier II
67 NC_000006.12 6 158024152 + SYNJ2 NNNTTGTCGGCCAGGCACGG NGG 2 0.015 Tier II
68 NC_000007.14 7 6033860 - EIF2AK1 NNNTTGTCGGCCAGGCACGG NGG 2 0.015 Tier II
69 NC_000007.14 7 6033860 - ANKRD61 NNNTTGTCGGCCAGGCACGG NGG 2 0.015 Tier II
70 NC_000014.9 14 96479368 - AK7 NNNTTGTCGGCCAGGCACGG NGG 2 0.015 Tier II
71 NC_000002.12 2 75550851 + EVA1A NNNTGGTCGGGCAGGCACGG NGG 2 0.0125 Tier II
72 NC_000001.11 1 6907764 - CAMTA1 NNNTGGTCTGCCAGTCACGG NGC 2 0.0119 Tier II
73 NC_000001.11 1 112642506 - CAPZA1 NNNTGGTCGGCCAGGCGCGG NGG 2 0.0088 Tier II
74 NC_000010.11 10 80353453 - DYDC2 NNNTGGTCGGCCAGGCGCGG NGG 2 0.0088 Tier II
75 NC_000010.11 10 80353453 - DYDC1 NNNTGGTCGGCCAGGCGCGG NGG 2 0.0088 Tier II
76 NC_000022.11 22 33306135 - LARGE1 NNNTGGTCGGCCAGGCGCGG NGG 2 0.0088 Tier II
77 NC_000002.12 2 26951781 + LOC124905979 NNNTGGTCGGCCAGTCACAG NGG 1 0.7143 Tier III
78 NC_000001.11 1 13488561 - LOC102724856 NNNTGTTCAGCCAGTCACGG NGG 2 0.4286 Tier III
79 NC_000022.11 22 35632235 + LOC284912 NNNGGGTGGGCCAGTCACGG NGG 2 0.4018 Tier III
80 NC_000022.11 22 18060054 - LOC105372853 NNNTGGGGGGCCAGTCACGG NGG 2 0.3782 Tier III
81 NC_000014.9 14 69193350 - GALNT16-AS1 NNNTGGTGGGCCAGACACGG NGG 2 0.3722 Tier III
82 NC_000010.11 10 118970989 - LINC03036 NNNTGGTCTGCCAGTCAAGG NGG 2 0.2872 Tier III
83 NC_000011.10 11 72528884 + LOC105369376 NNNTTGTTGGCCAGTCACGG NGG 2 0.2625 Tier III
84 NC_000012.12 12 114016539 + LOC105369993 NNNTTGCCGGCCAGTCACGG NGG 2 0.2063 Tier III
85 NC_000009.12 9 83831981 - LOC101927552 NNNTGATCGGCCAGGCACGG NGG 2 0.05 Tier III
86 NC_000002.12 2 150196434 + LINC01818 NNNTCGTCGGCCAGGCACGG NGG 2 0.0393 Tier III
87 NC_000018.10 18 73242452 - LINC02864 NNNTCGTCGGCCAGGCACGG NGG 2 0.0393 Tier III
88 NC_000012.12 12 53742169 - LOC105378250 NNNTGGCCGGCCAGGCACGG NGG 2 0.0344 Tier III
89 NC_000002.12 2 187071779 + CALCRL-AS1 NNNTGTTCGGCCAGGCACGG NGG 2 0.0333 Tier III
90 NC_000014.9 14 22741186 - OXA1L-DT NNNTGTTCGGCCAGGCACGG NGG 2 0.0333 Tier III
91 NC_000012.12 12 19571065 - LOC101928387 NNNTGGTCGGCCGGGCACGG NGG 2 0.0326 Tier III
92 NC_000007.14 7 102738721 - RASA4DP NNNTGGTAGGCCAGGCACGG NGG 2 0.0325 Tier III
93 NC_000012.12 12 16131648 - LOC101928362 NNNCGGTCGGCCAGGCACGG NGG 2 0.0324 Tier III
94 NC_000001.11 1 113015163 - LRIG2-DT NNNTGGTCAGCCAGGCACGG NGG 2 0.0321 Tier III
95 NC_000005.10 5 89279384 - MEF2C-AS1 NNNTGGTCGGCCAGGCATGG NGG 2 0.0321 Tier III
96 NC_000002.12 2 70059182 - PCBP1-AS1 NNNTGGTGGGCCAGGCACGG NGG 2 0.0321 Tier III
97 NC_000007.14 7 112618980 + LINC03076 NNNTGGTGGGCCAGGCACGG NGG 2 0.0321 Tier III
98 NC_000006.12 6 106699317 - LINC02526 NNNAGGTCGGCCAGGCACGG NGG 2 0.0238 Tier III
99 NC_000003.12 3 15961249 - LOC107986064 NNNTTGTCGGCCAGGCACGG NGG 2 0.015 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 91737604 + Trmt112-ps1 NNNTGGTCGGCGAGTCACTG NGG 2 0.2963 Tier III
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Other clones with same target sequence:

(none)