Construct: sgRNA BRDN0001147550

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTAAACATATACTAAGCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRAK4 (51135)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75665

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 43777690 - IRAK4 NNNAAACATATACTAAGCAG NGG 0 1.0 Tier I
2 NC_000002.12 2 64114001 + PELI1 NNNAAACAAATACTAAGCAA NGG 2 0.8705 Tier II
3 NC_000008.11 8 130244602 + ASAP1 NNNAAACATTTACTAAGCAA NGG 2 0.8272 Tier II
4 NC_000002.12 2 210661075 - CPS1 NNNAAATATATACTAAGCAA NGG 2 0.7617 Tier II
5 NC_000003.12 3 45488460 + LARS2 NNNAAACAAAAACTAAGCAG NGG 2 0.6964 Tier II
6 NC_000017.11 17 51795166 - CA10 NNNAAGCATTTACTAAGCAG NGG 2 0.6303 Tier II
7 NC_000003.12 3 143736406 + SLC9A9 NNNAAACATTTACTAAGCAT NGG 2 0.6176 Tier II
8 NC_000017.11 17 30050903 + EFCAB5 NNNAAACATTTACTAAGTAG NGG 2 0.5672 Tier II
9 NC_000005.10 5 37018220 - NIPBL NNNAAATATACACTAAGCAG NGG 2 0.5417 Tier II
10 NC_000002.12 2 230218542 + SP110 NNNAAACATATACTAAGAAG NGG 1 0.5385 Tier II
11 NC_000002.12 2 230218542 + SP140 NNNAAACATATACTAAGAAG NGG 1 0.5385 Tier II
12 NC_000013.11 13 28915314 + MTUS2 NNNGAATATATACTAAGCAG NGG 2 0.5078 Tier II
13 NC_000016.10 16 10395307 + ATF7IP2 NNNAAACATAAACAAAGCAG NGG 2 0.4643 Tier II
14 NC_000014.9 14 78360358 - NRXN3 NNNAAACATATATAAAGCAG NGG 2 0.4333 Tier II
15 NC_000006.12 6 114091703 + HS3ST5 NNNAAAAATGTACTAAGCAG NGG 2 0.4167 Tier II
16 NC_000008.11 8 125204080 - NSMCE2 NNNAAATATATACTAAGCAC NGG 2 0.3482 Tier II
17 NC_000003.12 3 173484185 + NLGN1 NNNAAAGATATACAAAGCAG NGG 2 0.2913 Tier II
18 NC_000021.9 21 43639405 + HSF2BP NNNAAACATATACTCAGCAA NGG 2 0.2557 Tier II
19 NC_000002.12 2 50628615 + NRXN1 NNNAAACAAATACTAAGCAG NAG 2 0.2407 Tier II
20 NC_000008.11 8 18775380 + PSD3 NNNAAATATATACCAAGCAG NGG 2 0.2321 Tier II
21 NC_000002.12 2 132744598 + NCKAP5 NNNAAACATAAACTAAGCAG NAG 2 0.1944 Tier II
22 NC_000002.12 2 178708650 - TTN NNNAAATATATACTTAGCAG NGG 2 0.1625 Tier II
23 NC_000005.10 5 172326632 + SH3PXD2B NNNAAATATATACTAGGCAG NGG 2 0.1563 Tier II
24 NC_000001.11 1 240337651 + FMN2 NNNAAACATAGAATAAGCAG NGG 2 0.1538 Tier II
25 NC_000007.14 7 11690084 - THSD7A NNNAAAAATATACTTAGCAG NGG 2 0.15 Tier II
26 NC_000013.11 13 97969486 - IPO5 NNNAAAAATATACTTAGCAG NGG 2 0.15 Tier II
27 NC_000018.10 18 28121088 - CDH2 NNNAAACATATTCTAAGCAC NGG 2 0.1429 Tier II
28 NC_000002.12 2 141940407 + LRP1B NNNAAACATCTACTAAGCGG NGG 2 0.125 Tier II
29 NC_000006.12 6 24327902 - DCDC2 NNNAAACATATATTAAGCAG NGA 2 0.0486 Tier II
30 NC_000014.9 14 49661866 + POLE2 NNNAAGCATATACTAAGCAG NTG 2 0.0278 Tier II
31 NC_000015.10 15 24863675 - SNRPN NNNAAACATATACAAAGCAG NGC 2 0.0138 Tier II
32 NC_000003.12 3 44008016 - LOC124909489 NNNAAACAAAAACTAAGCAG NGG 2 0.6964 Tier III
33 NC_000003.12 3 45488460 + LARS2-AS1 NNNAAACAAAAACTAAGCAG NGG 2 0.6964 Tier III
34 NC_000020.11 20 10901982 - LOC101929413 NNNAAACATAAACAAAGCAG NGG 2 0.4643 Tier III
35 NC_000005.10 5 108484623 - LOC105379114 NNNAAACAGATGCTAAGCAG NGG 2 0.4471 Tier III
36 NC_000006.12 6 114091703 + HDAC2-AS2 NNNAAAAATGTACTAAGCAG NGG 2 0.4167 Tier III
37 NC_000002.12 2 50628615 + LOC101927089 NNNAAACAAATACTAAGCAG NAG 2 0.2407 Tier III
38 NC_000012.12 12 102497017 + LINC02456 NNNAAACATATAAAAAGCAG NGG 2 0.2381 Tier III
39 NC_000002.12 2 178708650 - LOC124906100 NNNAAATATATACTTAGCAG NGG 2 0.1625 Tier III
40 NC_000002.12 2 59236270 + LINC01793 NNNAAACATATACTTAGCAG NAG 2 0.0519 Tier III
41 NC_000013.11 13 89440349 + LOC107984621 NNNAAACATATACTTACCAG NGG 2 0.0471 Tier III
42 NC_000015.10 15 24863675 - SNHG14 NNNAAACATATACAAAGCAG NGC 2 0.0138 Tier III
43 NC_000006.12 6 127093243 + LOC105377989 NNNAAAAATATACTATGCAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 8754697 - Sntg1 NNNAAACATAAACTAAACAG NGG 2 0.7 Tier II
2 NC_000073.6 7 87911702 - Grm5 NNNAAACATAAACTAAACAG NGG 2 0.7 Tier II
3 NT_165789.2 X 298589 + Tmlhe NNNAAACATAAACTAAACAG NGG 2 0.7 Tier II
4 NC_000071.6 5 63651971 + Nwd2 NNNAAACACACACTAAGCAG NGG 2 0.6154 Tier II
5 NC_000075.6 9 68995217 + Rora NNNAAACAAATACAAAGCAG NGG 2 0.5748 Tier II
6 NC_000067.6 1 45808644 - Wdr75 NNNAAACAGAAACTAAGCAG NGG 2 0.4643 Tier II
7 NC_000084.6 18 84461227 + Zfp407 NNNAAACAGAAACTAAGCAG NGG 2 0.4643 Tier II
8 NC_000069.6 3 20138938 + Gyg NNNAAACATAAACAAAGCAG NGG 2 0.4643 Tier II
9 NC_000067.6 1 193117828 - Utp25 NNNAAACATATACTGAGCAT NGG 2 0.455 Tier II
10 NC_000074.6 8 47043730 - Enpp6 NNNAAACATATACAAAGCAT NGG 2 0.4333 Tier II
11 NC_000074.6 8 104492046 + Terb1 NNNTAACATATACAAAGCAG NGG 2 0.3939 Tier II
12 NC_000070.6 4 150484312 - Rere NNNAAACGTAGACTAAGCAG NGG 2 0.2933 Tier II
13 NC_000079.6 13 36115448 - Lyrm4 NNNAAACAGAGACTAAGCAG NGG 2 0.2476 Tier II
14 NC_000068.7 2 156831345 + LOC115486128 NNNAAACATATAATAAGAAG NGG 2 0.2071 Tier II
15 NC_000079.6 13 63166565 - Aopep NNNAAACATATACTTAACAG NGG 2 0.1867 Tier II
16 NC_000070.6 4 115793498 - Atpaf1 NNNAAACATATACAAAGCAG NAG 2 0.1605 Tier II
17 NC_000076.6 10 90310725 - Anks1b NNNCAACATATACTAAGCAC NGG 2 0.1513 Tier II
18 NC_000067.6 1 40431404 + Il1rl1 NNNATACATATTCTAAGCAG NGG 2 0.1212 Tier II
19 NC_000071.6 5 135706040 + Por NNNAAACATCTTCTAAGCAG NGG 2 0.1111 Tier II
20 NC_000069.6 3 102176489 + Vangl1 NNNAAACATAGACTAACCAG NGG 2 0.0941 Tier II
21 NC_000073.6 7 74367442 - Slco3a1 NNNAAACATACACTAAGGAG NGG 2 0.0889 Tier II
22 NC_000078.6 12 3396234 - Kif3c NNNAAACATATACCAGGCAG NGG 2 0.0549 Tier II
23 NC_000076.6 10 63758773 - Ctnna3 NNNAAACATAAACTAAGCAG NGA 2 0.0521 Tier II
24 NC_000068.7 2 96384028 + Lrrc4c NNNAAACATATTGTAAGCAG NGG 2 0.0455 Tier II
25 NC_000084.6 18 77547994 - Rnf165 NNNTAACATATACTAAGCAG NGA 2 0.0442 Tier II
26 NC_000067.6 1 124805718 - Dpp10 NNNAAACATATACAAAGCAG NTG 2 0.0241 Tier II
27 NC_000069.6 3 114918350 - Olfm3 NNNAAACATATACGAAGCAA NGG 2 0.0 Tier II
28 NC_000075.6 9 28227766 - Opcml NNNAAACATATACTATGCAG NGA 2 0.0 Tier II
29 NC_000075.6 9 69276114 + Rora NNNAAACATATACTTCGCAG NGG 2 0.0 Tier II
30 NC_000074.6 8 68002412 + Psd3 NNNAAACATGTACTATGCAG NGG 2 0.0 Tier II
31 NC_000075.6 9 90914775 - Gm31114 NNNAAAAATATACTAAGCAA NGG 2 0.7031 Tier III
32 NC_000069.6 3 17702991 - Gm30340 NNNAAGAATATACTAAGCAG NGG 2 0.5357 Tier III
33 NC_000079.6 13 53749227 - Gm34439 NNNAAAAATATACAAAGCAG NGG 2 0.4643 Tier III
34 NC_000070.6 4 60049787 - Mup-ps1 NNNAAACAGAAACTAAGCAG NGG 2 0.4643 Tier III
35 NC_000068.7 2 156831345 + Gm14230 NNNAAACATATAATAAGAAG NGG 2 0.2071 Tier III
36 NC_000079.6 13 28412934 + Gm40841 NNNAAAAATATACTAAGGAG NGG 2 0.1 Tier III
37 NC_000074.6 8 68002412 + Gm15991 NNNAAACATGTACTATGCAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)