Construct: sgRNA BRDN0001147566

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCCCACCATAAGAACCCCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1G1 (53944)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77439

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 64300394 + CSNK1G1 NNNCACCATAAGAACCCCAG NGG 0 1.0 Tier I
2 NC_000017.11 17 74930724 + OTOP2 NNNCACCATAAGAACCCCAC NCG 2 0.0459 Tier I
3 NC_000017.11 17 7292686 + YBX2 NNNTATCATAAGAACCCCAG NGG 2 0.7429 Tier II
4 NC_000004.12 4 53110290 + SCFD2 NNNCACCATAAAAACCCTAG NGG 2 0.6 Tier II
5 NC_000017.11 17 50102767 - PDK2 NNNCTCCACAAGAACCCCAG NGG 2 0.3357 Tier II
6 NC_000001.11 1 44270735 + ERI3 NNNCACTATAAGAACTCCAG NGG 2 0.25 Tier II
7 NC_000017.11 17 79590196 + RBFOX3 NNNCAGGATAAGAACCCCAG NGG 2 0.2353 Tier II
8 NC_000010.11 10 87544388 - MINPP1 NNNCTCCATAAGAACCCAAG NGG 2 0.1958 Tier II
9 NC_000017.11 17 3692472 - P2RX5 NNNCACCATCACAACCCCAG NGG 2 0.1765 Tier II
10 NC_000002.12 2 182202640 + PDE1A NNNCATCATAAGAACCCCAG NGA 2 0.0645 Tier II
11 NC_000019.10 19 45342936 - KLC3 NNNCACCATAAGCAGCCCAG NGG 2 0.0105 Tier II
12 NC_000019.10 19 47438192 - SLC8A2 NNNCACCATACGAACCCCAG NGC 2 0.0089 Tier II
13 NC_000001.11 1 204456488 + PIK3C2B NNNCACCATAAGAATCCCAG NGA 2 0.0046 Tier II
14 NC_000006.12 6 74437525 - LOC101928516 NNNCACCATAAAAACCCTAG NGG 2 0.6 Tier III
15 NC_000017.11 17 3692472 - P2RX5-TAX1BP3 NNNCACCATCACAACCCCAG NGG 2 0.1765 Tier III
16 NC_000015.10 15 66935381 - SMASR NNNCACTATAAGAAGCCCAG NGG 2 0.0406 Tier III
17 NC_000019.10 19 31839132 + LINC01837 NNNCACCATATGAATCCCAG NGG 2 0.0205 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 65958647 - Csnk1g1 NNNCACCATGAGAACCCCAG NGG 1 0.5556 Tier I
2 NC_000083.6 17 78224952 - Crim1 NNNCACCATTAGAACCCCAG NGG 1 0.8824 Tier II
3 NC_000080.6 14 4047348 + Gm32966 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
4 NC_000080.6 14 4139157 + Gm32966 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
5 NC_000080.6 14 4676973 + Gm3239 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
6 NC_000080.6 14 4753588 + Gm26650 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
7 NC_000080.6 14 4790784 + Gm26650 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
8 NC_000080.6 14 4882995 + Gm26650 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
9 NC_000080.6 14 5432088 - Gm3194 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
10 NC_000080.6 14 5933181 - Gm3248 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
11 NC_000080.6 14 6011531 - LOC115488283 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
12 NC_000080.6 14 6522841 - LOC108168187 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
13 NC_000080.6 14 7077219 - Gm3696 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
14 NC_000080.6 14 7077219 - Gm31035 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier II
15 NC_000085.6 19 31054496 + Prkg1 NNNCACCATAAGAGCACCAG NGG 2 0.4667 Tier II
16 NC_000074.6 8 27089320 + Adgra2 NNNCACCAGAAGGACCCCAG NGG 2 0.4037 Tier II
17 NC_000079.6 13 19092086 - Amph NNNAACCATAAGAACCACAG NGG 2 0.393 Tier II
18 NC_000073.6 7 44078529 - Klk1b21 NNNCCCCATAAGAACCCCAT NGG 2 0.35 Tier II
19 NC_000078.6 12 24584800 - Grhl1 NNNCAGCATAAGGACCCCAG NGG 2 0.3261 Tier II
20 NC_000075.6 9 22436551 + 9530077C05Rik NNNCATCATCAGAACCCCAG NGG 2 0.3095 Tier II
21 NC_000068.7 2 7360657 - Celf2 NNNCATCATAAGAAACCCAG NGG 2 0.2063 Tier II
22 NC_000083.6 17 6018946 - Synj2 NNNCACCATAGGAACTCCAG NGG 2 0.2 Tier II
23 NC_000076.6 10 77835029 + Tspear NNNCACCATCAGAACCACAG NGG 2 0.1556 Tier II
24 NC_000075.6 9 31454626 + Tmem45b NNNCACCATGAGCACCCCAG NGG 2 0.117 Tier II
25 NC_000085.6 19 37587163 + Exoc6 NNNCACCCTAAGAACCCCCG NGG 2 0.0887 Tier II
26 NC_000084.6 18 6628019 - 4921524L21Rik NNNCACCATCAGCACCCCAG NGG 2 0.0702 Tier II
27 NC_000076.6 10 70181365 - Ccdc6 NNNCACCATAAGAACACCAG NTG 2 0.039 Tier II
28 NC_000080.6 14 122131491 - Tm9sf2 NNNCACCATAAGAAGCCCAT NGG 2 0.035 Tier II
29 NC_000082.6 16 63760833 - Epha3 NNNCACCATATGAACCCCAG NGA 2 0.0214 Tier II
30 NC_000068.7 2 68266868 - Stk39 NNNCACCATATGAATCCCAG NGG 2 0.0205 Tier II
31 NC_000080.6 14 4449433 + Gm3164 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier III
32 NC_000080.6 14 5176392 + Gm26552 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier III
33 NC_000080.6 14 5359812 - Gm41087 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier III
34 NC_000080.6 14 5712354 - Gm36476 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier III
35 NC_000080.6 14 5933181 - Gm51421 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier III
36 NC_000080.6 14 6011531 - Gm51421 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier III
37 NC_000080.6 14 6399734 - Gm26630 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier III
38 NC_000080.6 14 6729628 - Gm26680 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier III
39 NC_000080.6 14 6862393 - Gm32041 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier III
40 NC_000080.6 14 6959425 - Gm32041 NNNCACCATAACAACCCCAG NGG 1 0.5294 Tier III
41 NC_000074.6 8 27089320 + Gm39151 NNNCACCAGAAGGACCCCAG NGG 2 0.4037 Tier III
42 NC_000080.6 14 6826246 - Gm26680 NNNCACCCTAACAACCCCAG NGG 2 0.2269 Tier III
43 NC_000079.6 13 78577183 + Gm32067 NNNCACCATAAGAACCACAG NAG 2 0.121 Tier III
44 NC_000081.6 15 8742509 + Gm31340 NNNCACCATAAGACCCTCAG NGG 2 0.1 Tier III
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Other clones with same target sequence:

(none)