Construct: sgRNA BRDN0001147621
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGCAGAGTGGTCACCCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDC42BPG (55561)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77526
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
64840596 |
+ |
CDC42BPG |
NNNGCAGAGTGGTCACCCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
169542095 |
- |
F5 |
NNNGCAGAGTGGCCACCCAA |
NGT |
2 |
0.0127 |
Tier I |
| 3 |
NC_000009.12 |
9 |
109458187 |
+ |
PTPN3 |
NNNGCACAGTGGACACCCAA |
NGG |
2 |
0.476 |
Tier II |
| 4 |
NC_000017.11 |
17 |
8151145 |
- |
PER1 |
NNNGCAGAGTGGACACCTAA |
NGG |
2 |
0.4451 |
Tier II |
| 5 |
NC_000001.11 |
1 |
181519416 |
+ |
CACNA1E |
NNNGCAGAGTGGTTACCAAA |
NGG |
2 |
0.3949 |
Tier II |
| 6 |
NC_000010.11 |
10 |
127954265 |
- |
PTPRE |
NNNGCAGAGTGGTCACTCAG |
NGG |
2 |
0.3569 |
Tier II |
| 7 |
NC_000014.9 |
14 |
22995337 |
- |
C14orf93 |
NNNGCAGATTGGTCACCAAA |
NGG |
2 |
0.2872 |
Tier II |
| 8 |
NC_000003.12 |
3 |
72425819 |
+ |
RYBP |
NNNGCAGATTGTTCACCCAA |
NGG |
2 |
0.2051 |
Tier II |
| 9 |
NC_000005.10 |
5 |
126871456 |
- |
MARCHF3 |
NNNGCACAGTGGGCACCCAA |
NGG |
2 |
0.1793 |
Tier II |
| 10 |
NC_000012.12 |
12 |
2678180 |
+ |
CACNA1C |
NNNGCAGAGTGGTCACCCCT |
NGG |
2 |
0.1241 |
Tier II |
| 11 |
NC_000001.11 |
1 |
23084034 |
- |
LUZP1 |
NNNGCAGACTGGTCTCCCAA |
NGG |
2 |
0.1077 |
Tier II |
| 12 |
NC_000023.11 |
X |
53397211 |
+ |
SMC1A |
NNNGAAGAGTGGTCAGCCAA |
NGG |
2 |
0.0879 |
Tier II |
| 13 |
NC_000016.10 |
16 |
78751907 |
+ |
WWOX |
NNNGCAGAGTGGTCACCCAG |
NTG |
2 |
0.0298 |
Tier II |
| 14 |
NC_000014.9 |
14 |
22995337 |
- |
AJUBA-DT |
NNNGCAGATTGGTCACCAAA |
NGG |
2 |
0.2872 |
Tier III |
| 15 |
NC_000016.10 |
16 |
27273965 |
+ |
NSMCE1-DT |
NNNGCAGAGTGGTCCCCCAT |
NGG |
2 |
0.1636 |
Tier III |
| 16 |
NC_000012.12 |
12 |
2678180 |
+ |
CACNA1C-AS1 |
NNNGCAGAGTGGTCACCCCT |
NGG |
2 |
0.1241 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
145569161 |
+ |
Slc24a3 |
NNNGCTGAGTGGTTACCCAA |
NGG |
2 |
0.5238 |
Tier II |
| 2 |
NC_000077.6 |
11 |
114048114 |
+ |
Sdk2 |
NNNGCAGAGGGGTCAACCAA |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000068.7 |
2 |
163733201 |
- |
Ada |
NNNGCAAAGTGGTCACACAA |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000081.6 |
15 |
84687876 |
- |
Prr5 |
NNNGGAGAGTGGTCACCCAG |
NGG |
2 |
0.4588 |
Tier II |
| 5 |
NC_000078.6 |
12 |
33287681 |
- |
Atxn7l1 |
NNNGCAGAGTGCTCACCTAA |
NGG |
2 |
0.3403 |
Tier II |
| 6 |
NC_000075.6 |
9 |
119053949 |
- |
Vill |
NNNGCACAGTGGTCACACAA |
NGG |
2 |
0.3208 |
Tier II |
| 7 |
NC_000070.6 |
4 |
137957971 |
- |
Ece1 |
NNNGGAGAGTGGTCACTCAA |
NGG |
2 |
0.28 |
Tier II |
| 8 |
NC_000067.6 |
1 |
181599707 |
- |
Dnah14 |
NNNGCAGTGTGGTCACACAA |
NGG |
2 |
0.2 |
Tier II |
| 9 |
NC_000067.6 |
1 |
88319127 |
- |
Trpm8 |
NNNGCAGATTGGTCACCCAA |
NAG |
2 |
0.1383 |
Tier II |
| 10 |
NC_000085.6 |
19 |
23365623 |
+ |
Mamdc2 |
NNNGGAGAGTGGTCACCCAA |
NTG |
2 |
0.0234 |
Tier II |
| 11 |
NC_000080.6 |
14 |
20075903 |
+ |
Saysd1 |
NNNGCAGAGTGCTCACCCAA |
NGC |
2 |
0.0118 |
Tier II |
| 12 |
NC_000082.6 |
16 |
14598898 |
+ |
A630010A05Rik |
NNNGCAGAGTTCTCACCCAA |
NGG |
2 |
0.2036 |
Tier III |
| 13 |
NC_000069.6 |
3 |
101503536 |
+ |
Gm40109 |
NNNGCAGAGTGGTCAGCCCA |
NGG |
2 |
0.0318 |
Tier III |
Other clones with same target sequence:
(none)
