Construct: sgRNA BRDN0001147707
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGTAACTATGATAATCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAR2B (5577)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76152
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
107146372 |
+ |
PRKAR2B |
NNNGTAACTATGATAATCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
34994519 |
+ |
ANKS1A |
NNNGTAACTATGATATTCCT |
NGG |
2 |
0.0 |
Tier II |
| 3 |
NC_000015.10 |
15 |
27217662 |
- |
GABRG3 |
NNNTTAACTATGATACTCGT |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000015.10 |
15 |
66654530 |
+ |
LINC01169 |
NNNGTAACGATCATAATCGT |
NGG |
2 |
0.3277 |
Tier III |
| 5 |
NC_000001.11 |
1 |
149669433 |
- |
LINC00869 |
NNNGTAACTATGAGAATTGT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
31972140 |
- |
Prkar2b |
NNNGTAACTATGATAACCGT |
NGG |
1 |
0.7059 |
Tier I |
| 2 |
NC_000086.7 |
X |
10592654 |
- |
Tspan7 |
NNNGTAACTATCAAAATCGT |
NGG |
2 |
0.3277 |
Tier II |
| 3 |
NC_000084.6 |
18 |
31657301 |
+ |
Sap130 |
NNNGTAACTATGATTATTGT |
NGG |
2 |
0.1286 |
Tier II |
| 4 |
NC_000079.6 |
13 |
114017853 |
- |
Arl15 |
NNNGTAACTATGAGAATTGT |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000079.6 |
13 |
81059733 |
- |
9330111N05Rik |
NNNGTAACTATGATAAACAT |
NGG |
2 |
0.381 |
Tier III |
| 6 |
NC_000067.6 |
1 |
28854527 |
- |
Gm45942 |
NNNGTAACTATACTAATCGT |
NGG |
2 |
0.1965 |
Tier III |
| 7 |
NC_000068.7 |
2 |
42903616 |
+ |
Gm39803 |
NNNGTAACTATGATAATAGT |
NGT |
2 |
0.0087 |
Tier III |
Other clones with same target sequence:
(none)
