Construct: sgRNA BRDN0001147709

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCAAAGTCTGTGGCAATGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROCK1 (6093)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77593

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000018.10 18 20991181 - ROCK1 NNNAAGTCTGTGGCAATGTG NGG 0 1.0 Tier I
2 NC_000017.11 17 48212685 + SKAP1 NNNAAGTCTATGGCAATGTT NGG 2 0.6533 Tier II
3 NC_000023.11 X 17390579 + NHS NNNAAGTTTGCGGCAATGTG NGG 2 0.5833 Tier II
4 NC_000020.11 20 1937772 - SIRPA NNNAGGTATGTGGCAATGTG NGG 2 0.468 Tier II
5 NC_000003.12 3 25577532 - RARB NNNAATTCTGTGGCAATGTT NGG 2 0.4667 Tier II
6 NC_000022.11 22 30544276 - SEC14L6 NNNAGGTGTGTGGCAATGTG NGG 2 0.4629 Tier II
7 NC_000021.9 21 17624275 - CXADR NNNAAGTGTGTGGCAATTTG NGG 2 0.4286 Tier II
8 NC_000011.10 11 34204247 + ABTB2 NNNAGGTCTGTGGCAAAGTG NGG 2 0.384 Tier II
9 NC_000011.10 11 12882068 - TEAD1 NNNAAGTCAGGGGCAATGTG NGG 2 0.3714 Tier II
10 NC_000011.10 11 126926367 - KIRREL3 NNNCAGTCAGTGGCAATGTG NGG 2 0.3277 Tier II
11 NC_000001.11 1 6418045 - HES2 NNNGAGTCTGTGCCAATGTG NGG 2 0.2632 Tier II
12 NC_000006.12 6 53337825 + ELOVL5 NNNAAGGCTGGGGCAATGTG NGG 2 0.2353 Tier II
13 NC_000001.11 1 181437173 - CACNA1E NNNAAGTCTGTGGCAATAAG NGG 2 0.1978 Tier II
14 NC_000008.11 8 66752357 + SGK3 NNNAAGTCTGGGGCAATCTG NGG 2 0.1905 Tier II
15 NC_000008.11 8 66752357 + C8orf44-SGK3 NNNAAGTCTGGGGCAATCTG NGG 2 0.1905 Tier II
16 NC_000010.11 10 33281635 - NRP1 NNNAAGTCAGTGGCAGTGTG NGG 2 0.1786 Tier II
17 NC_000007.14 7 98975115 + TRRAP NNNAAGTGTGTGGCCATGTG NGG 2 0.1753 Tier II
18 NC_000015.10 15 62472946 + TLN2 NNNAAGTCTGTGGCCATGTC NGG 2 0.1169 Tier II
19 NC_000016.10 16 4958918 + SEC14L5 NNNAAGTCTGTGGCAAGCTG NGG 2 0.056 Tier II
20 NC_000006.12 6 143292663 + AIG1 NNNAAGTCTGTGGCAATGTG NCA 2 0.0 Tier II
21 NC_000013.11 13 39260014 + LOC107984579 NNNAAGTTTGTGGCAACGTG NGG 2 0.6176 Tier III
22 NC_000007.14 7 127587694 + LOC105375490 NNNAAGTCTGTGGCAAAGTG NGG 1 0.5333 Tier III
23 NC_000022.11 22 30544276 - SIRPAP1 NNNAGGTGTGTGGCAATGTG NGG 2 0.4629 Tier III
24 NC_000011.10 11 126926367 - LOC105369559 NNNCAGTCAGTGGCAATGTG NGG 2 0.3277 Tier III
25 NC_000006.12 6 9855586 - OFCC1 NNNAAGTCTGAGGAAATGTG NGG 2 0.2625 Tier III
26 NC_000002.12 2 218922539 - LINC01494 NNNAAGCCTGTGGAAATGTG NGG 2 0.2406 Tier III
27 NC_000002.12 2 72024359 - LOC105374798 NNNAAGTCTGTAGCAATGGG NGG 2 0.2333 Tier III
28 NC_000003.12 3 45205389 + LOC101928636 NNNAAGGCTGTGGAAATGTG NGG 2 0.2059 Tier III
29 NC_000011.10 11 31830588 - PAX6-AS1 NNNAAGTCTGTGTCAAAGTG NGG 2 0.16 Tier III
30 NC_000016.10 16 48744457 - LOC105371240 NNNATGTCTGTGTCAATGTG NGG 2 0.1091 Tier III
31 NC_000019.10 19 19941023 + LOC124904665 NNNAAGTCTGGGGCAGTGTG NGG 2 0.0769 Tier III
32 NC_000007.14 7 90101277 + STEAP2-AS1 NNNAAGTCTGTGGCAAAGTG NTG 2 0.0208 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 75025215 + Gab3 NNNAAGTCTGTGCCAATGAG NGG 2 0.1203 Tier I
2 NC_000084.6 18 10099262 - Rock1 NNNAAGTCTGTTGCCATGTG NGG 2 0.1049 Tier I
3 NC_000085.6 19 4151500 + Coro1b NNNATGTCTGTGGCAAGGTG NGG 2 0.0428 Tier I
4 NC_000073.6 7 112851283 + Tead1 NNNAAGGCTATGGCAATGTG NGG 2 0.549 Tier II
5 NC_000067.6 1 13583317 - Tram1 NNNAGGTCTGTGGCAAAGTG NGG 2 0.384 Tier II
6 NC_000082.6 16 85058895 + App NNNAAGTCTGTGAAAATGTG NGG 2 0.3231 Tier II
7 NC_000081.6 15 48161460 - Csmd3 NNNAAGTATGTGGAAATGTG NGG 2 0.2275 Tier II
8 NC_000081.6 15 12875870 + Drosha NNNAAGTCTGTGGCAGTATG NGG 2 0.1331 Tier II
9 NC_000068.7 2 154566181 - E2f1 NNNGAGTCTGTGGCAGTGTG NGG 2 0.1202 Tier II
10 NC_000076.6 10 43941378 - Rtn4ip1 NNNAAGTCTGTGGCAGTGAG NGG 2 0.0549 Tier II
11 NC_000086.7 X 36437736 + Gm14569 NNNAACTCTGTGGCAATGTG NGA 2 0.0473 Tier II
12 NC_000078.6 12 44331905 + Nrcam NNNAAGTATGTGGCAATGTG NGA 2 0.0451 Tier II
13 NC_000074.6 8 91095006 - Rbl2 NNNAAGTGTGTGGCAATGTG NGC 2 0.0143 Tier II
14 NC_000071.6 5 5628284 + Cfap69 NNNATGTCTGTGGCAATGTG NGC 2 0.0081 Tier II
15 NC_000069.6 3 63309433 + Mme NNNAAGACTGTGGCACTGTG NGG 2 0.0 Tier II
16 NC_000077.6 11 119753650 - Rptor NNNAAGACTGTGGCATTGTG NGG 2 0.0 Tier II
17 NC_000073.6 7 94606525 + Gm32647 NNNAAGTCTGTGGTAATTTG NGG 2 0.4889 Tier III
18 NC_000083.6 17 82786919 - Gm34265 NNNAAGTTTCTGGCAATGTG NGG 2 0.35 Tier III
19 NC_000078.6 12 109516249 - Gm34081 NNNAAGCCTGTGGCAATCTG NGG 2 0.3274 Tier III
20 NC_000081.6 15 97107331 - Gm32733 NNNAAGTCTGTGGCAAAGTG NGA 2 0.037 Tier III
21 NC_000067.6 1 177557171 + Gm30013 NNNAAGTCTGTGGCAATGTG NCT 2 0.0 Tier III
22 NC_000068.7 2 38673496 + Adgrd2-ps NNNAAGTCTGTGGCACTGTG NGC 2 0.0 Tier III
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Other clones with same target sequence:

(none)