Construct: sgRNA BRDN0001147719

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATGATGGCAAAGTAGATGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAOK1 (57551)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76808

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 29482255 + TAOK1 NNNATGGCAAAGTAGATGTG NGG 0 1.0 Tier I
2 NC_000017.11 17 33807021 - ASIC2 NNNATGACAAAGCAGATGTG NGG 2 0.7895 Tier II
3 NC_000001.11 1 107950850 + VAV3 NNNATGGCTTAGTAGATGTG NGG 2 0.5294 Tier II
4 NC_000012.12 12 107443581 + ABTB3 NNNATGGCAGAGTAAATGTG NGG 2 0.5229 Tier II
5 NC_000003.12 3 120634436 - HGD NNNATGGGTAAGTAGATGTG NGG 2 0.3857 Tier II
6 NC_000005.10 5 20307346 + CDH18 NNNAGGGCAGAGTAGATGTG NGG 2 0.3556 Tier II
7 NC_000010.11 10 84215752 - CDHR1 NNNTTGGCAGAGTAGATGTG NGG 2 0.3535 Tier II
8 NC_000022.11 22 35813918 + RBFOX2 NNNATGGGAAAGTAGAAGTG NGG 2 0.3429 Tier II
9 NC_000007.14 7 136875369 + CHRM2 NNNATGGCAAACTAGAAGTG NGG 2 0.2824 Tier II
10 NC_000022.11 22 50435794 + PPP6R2 NNNAGGGCAAAGTAGATGGG NGG 2 0.16 Tier II
11 NC_000007.14 7 50480363 - DDC NNNATGGCAAAGTACAAGTG NGG 2 0.1455 Tier II
12 NC_000001.11 1 220568247 + MARK1 NNNAAGGCAAAGGAGATGTG NGG 2 0.1304 Tier II
13 NC_000017.11 17 65663364 + CEP112 NNNATGGCAAAGTAGATGTT NGA 2 0.0486 Tier II
14 NC_000015.10 15 68064780 + PIAS1 NNNATGGAAAAGTAGATGTG NGA 2 0.0451 Tier II
15 NC_000012.12 12 45935047 + SCAF11 NNNATGGAAAAGTAGATGTG NTG 2 0.0253 Tier II
16 NC_000010.11 10 91828908 + TNKS2 NNNATGACAAAGTAGATGTG NGT 2 0.0161 Tier II
17 NC_000007.14 7 136924938 - CHRM2 NNNATGGCAGAGTAGATGTG NGT 2 0.009 Tier II
18 NC_000020.11 20 51553297 - NFATC2 NNNAGGGCAAAGTAGTTGTG NGG 2 0.0 Tier II
19 NC_000009.12 9 117246411 + ASTN2 NNNATGGCAAACTAGTTGTG NGG 2 0.0 Tier II
20 NC_000011.10 11 14292440 - RRAS2 NNNATGGCAAAGTAGATGTG NAT 2 0.0 Tier II
21 NC_000002.12 2 117865692 + HTR5BP NNNAGGGCAAAGTAAATGTG NGG 2 0.6024 Tier III
22 NC_000011.10 11 42055848 - LINC02745 NNNATGGAAAAGCAGATGTG NGG 2 0.5132 Tier III
23 NC_000005.10 5 20307346 + CDH18-AS1 NNNAGGGCAGAGTAGATGTG NGG 2 0.3556 Tier III
24 NC_000004.12 4 139221823 + LOC105379412 NNNATGGCAAAGTAGGTTTG NGG 2 0.1282 Tier III
25 NC_000001.11 1 208872362 - LOC107985255 NNNAAGGCAAAGTAGATGTG NGA 2 0.0347 Tier III
26 NC_000010.11 10 61479473 - TMEM26-AS1 NNNATGGCAAAGAAGATGTG NTG 2 0.027 Tier III
27 NC_000010.11 10 61479473 - LOC124902548 NNNATGGCAAAGAAGATGTG NTG 2 0.027 Tier III
28 NC_000018.10 18 49735563 + LOC105372112 NNNATGGCAAAGTAGATGGG NTG 2 0.0097 Tier III
29 NC_000007.14 7 136924938 - LOC349160 NNNATGGCAGAGTAGATGTG NGT 2 0.009 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 77571641 - Taok1 NNNATGGCAAAGTTGATGTA NGG 2 0.5 Tier I
2 NC_000076.6 10 28761390 - Themis NNNATGGCAAAGTAAATGAG NGG 2 0.2689 Tier I
3 NC_000073.6 7 126878757 - Taok2 NNNATGGCAAAGTGGATGTC NGG 2 0.2 Tier I
4 NC_000067.6 1 86162791 - Armc9 NNNATGGCAAAGTGGATGTG NAG 2 0.121 Tier I
5 NC_000070.6 4 137499931 + Hspg2 NNNATGGCTAAGTAGATGTA NGG 2 0.5625 Tier II
6 NC_000073.6 7 3338857 - Cacng7 NNNATGGAAAAGCAGATGTG NGG 2 0.5132 Tier II
7 NC_000073.6 7 143297864 - Kcnq1 NNNTTGGCAAAGCAGATGTG NGG 2 0.5024 Tier II
8 NC_000067.6 1 168316010 + Pbx1 NNNATGGAAAGGTAGATGTG NGG 2 0.4225 Tier II
9 NC_000068.7 2 32789192 - Stxbp1 NNNATGGAAAGGTAGATGTG NGG 2 0.4225 Tier II
10 NC_000070.6 4 36332667 - Lingo2 NNNATGGCAAATTAGATGTA NGG 2 0.3606 Tier II
11 NC_000073.6 7 26761075 - Cyp2b19 NNNGTGGCAGAGTAGATGTG NGG 2 0.3472 Tier II
12 NC_000067.6 1 159732522 + Tnr NNNATGGCTGAGTAGATGTG NGG 2 0.3333 Tier II
13 NC_000083.6 17 7286215 + Rps6ka2 NNNATGGCTGAGTAGATGTG NGG 2 0.3333 Tier II
14 NC_000068.7 2 49161955 + Mbd5 NNNATGGCAAAGCACATGTG NGG 2 0.2153 Tier II
15 NC_000067.6 1 129495553 + Thsd7b NNNAAGGCAAAGTAGATGTC NGG 2 0.2143 Tier II
16 NC_000068.7 2 162313979 - Ptprt NNNATGTCACAGTAGATGTG NGG 2 0.1905 Tier II
17 NC_000075.6 9 20697903 + Olfm2 NNNATGGCAAAGAACATGTG NGG 2 0.1888 Tier II
18 NC_000083.6 17 80448422 - Sos1 NNNATGGGAAAGTAGATGCG NGG 2 0.1773 Tier II
19 NC_000067.6 1 60969336 - Icos NNNATGGTAAAGTAGGTGTG NGG 2 0.1683 Tier II
20 NC_000068.7 2 91949316 - Dgkz NNNATGGAAAAGTAGATGGG NGG 2 0.1625 Tier II
21 NC_000079.6 13 73924143 + Trip13 NNNAGGGCAAAGTAGATGGG NGG 2 0.16 Tier II
22 NC_000067.6 1 100044822 - Cntnap5b NNNATGGCAAAGTAGATGTA NGA 2 0.0651 Tier II
23 NC_000079.6 13 63396177 - Fancc NNNATGGCAAACTAGATGTG NCG 2 0.0567 Tier II
24 NC_000078.6 12 87298342 + Ism2 NNNATGGCCAAGTAGATGTG NGC 2 0.0127 Tier II
25 NC_000080.6 14 117431381 + Gpc6 NNNATGGCAAAGTATATGTG NGA 2 0.0099 Tier II
26 NC_000073.6 7 26761075 - Gm51442 NNNGTGGCAGAGTAGATGTG NGG 2 0.3472 Tier III
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Other clones with same target sequence:

(none)