Construct: sgRNA BRDN0001147723
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGGGTTTATCCGCCCCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80207
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
36394935 |
+ |
PRR5L |
NNNGGGTTTATCTGCCCCCA |
NAG |
2 |
0.1815 |
Tier II |
| 2 |
NC_000017.11 |
17 |
79611544 |
- |
RBFOX3 |
NNNGGGTTGATCCGCCCCCA |
NGA |
2 |
0.043 |
Tier II |
| 3 |
NC_000001.11 |
1 |
216895047 |
- |
ESRRG |
NNNGGGTTTATCCGCCCCCC |
NGC |
2 |
0.0051 |
Tier II |
| 4 |
NC_000005.10 |
5 |
74399995 |
+ |
LINC01331 |
NNNGAGTTTCTCCGCCCCCA |
NGG |
2 |
0.2889 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
116728679 |
+ |
Cdyl2 |
NNNGGGTTGTTCCGCCCCCA |
NGG |
2 |
0.5462 |
Tier II |
| 2 |
NC_000082.6 |
16 |
5380997 |
- |
Gm41414 |
NNNGGGTTTATCTGCTCCCA |
NGG |
2 |
0.2154 |
Tier III |
| 3 |
NC_000080.6 |
14 |
123170762 |
+ |
AA536875 |
NNNGGGTTTATCAGCCACCA |
NGG |
2 |
0.1795 |
Tier III |
| 4 |
NC_000080.6 |
14 |
123170762 |
+ |
Gm33906 |
NNNGGGTTTATCAGCCACCA |
NGG |
2 |
0.1795 |
Tier III |
Other clones with same target sequence:
(none)
