Construct: sgRNA BRDN0001147758
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGTTCATTCCAGGCCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MUSK (4593)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77502
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
110767961 |
- |
MUSK |
NNNGTTCATTCCAGGCCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
110443110 |
+ |
TMEM232 |
NNNGTTCATTCAAGGCCGTG |
NGG |
1 |
0.7143 |
Tier II |
| 3 |
NC_000005.10 |
5 |
14728319 |
+ |
ANKH |
NNNGTTCAATCCAGGCCTTG |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000004.12 |
4 |
3465062 |
+ |
DOK7 |
NNNGTTCATTCCAAGCCTTG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000015.10 |
15 |
56106829 |
+ |
RFX7 |
NNNGGTCATTCCAGGCCATG |
NGG |
2 |
0.4431 |
Tier II |
| 6 |
NC_000019.10 |
19 |
15792801 |
+ |
OR10H5 |
NNNGTTTATTCCAGGCCCTG |
NGG |
2 |
0.3869 |
Tier II |
| 7 |
NC_000023.11 |
X |
17667492 |
- |
NHS |
NNNGTGCATTCCAGGCCCTG |
NGG |
2 |
0.2721 |
Tier II |
| 8 |
NC_000004.12 |
4 |
2060803 |
+ |
NAT8L |
NNNGCTCATTCCAGGCGGTG |
NGG |
2 |
0.0588 |
Tier II |
| 9 |
NC_000001.11 |
1 |
183964284 |
- |
COLGALT2 |
NNNGTTCATTCCAGGCCATG |
NGT |
2 |
0.0112 |
Tier II |
| 10 |
NC_000005.10 |
5 |
35029805 |
- |
AGXT2 |
NNNGTTCATTCCAGGCCCTG |
NGT |
2 |
0.0077 |
Tier II |
| 11 |
NC_000009.12 |
9 |
110767961 |
- |
LOC107987115 |
NNNGTTCATTCCAGGCCGTG |
NGG |
0 |
1.0 |
Tier III |
| 12 |
NC_000005.10 |
5 |
14728319 |
+ |
LOC124900944 |
NNNGTTCAATCCAGGCCTTG |
NGG |
2 |
0.619 |
Tier III |
| 13 |
NC_000022.11 |
22 |
10687840 |
- |
LOC124905154 |
NNNGTTAATTCCAGGCCATG |
NGG |
2 |
0.5192 |
Tier III |
| 14 |
NC_000001.11 |
1 |
36695364 |
- |
LOC107984941 |
NNNGTTCAATCCAGGCAGTG |
NGG |
2 |
0.4333 |
Tier III |
| 15 |
NC_000023.11 |
X |
51369288 |
- |
LOC105373204 |
NNNGTTCATTCAAGGCCCTG |
NGG |
2 |
0.3401 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
38992031 |
- |
Igsf11 |
NNNGGTCATGCCAGGCCGTG |
NGG |
2 |
0.32 |
Tier II |
| 2 |
NC_000068.7 |
2 |
102073277 |
- |
Ldlrad3 |
NNNGTTCATGCCAGGCAGTG |
NGG |
2 |
0.2333 |
Tier II |
| 3 |
NC_000071.6 |
5 |
121429425 |
- |
Naa25 |
NNNGTTCATTTCAAGCCGTG |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000069.6 |
3 |
9496939 |
+ |
Zfp704 |
NNNGTTCAGTTCAGGCCGTG |
NGG |
2 |
0.1905 |
Tier II |
| 5 |
NC_000084.6 |
18 |
53519611 |
+ |
Prdm6 |
NNNGTTCATTTCAGGCAGTG |
NGG |
2 |
0.1436 |
Tier II |
| 6 |
NC_000073.6 |
7 |
137012865 |
+ |
Mgmt |
NNNGTTCATTCCAGGCCATG |
NTG |
2 |
0.027 |
Tier II |
| 7 |
NC_000075.6 |
9 |
27101578 |
+ |
Jam3 |
NNNGTTCATTCCAGCCCGTG |
NGT |
2 |
0.0044 |
Tier II |
| 8 |
NC_000077.6 |
11 |
113165427 |
+ |
2610035D17Rik |
NNNCTTCATTCCAGGACGTG |
NGG |
2 |
0.5294 |
Tier III |
| 9 |
NC_000077.6 |
11 |
113165427 |
+ |
LOC115487747 |
NNNCTTCATTCCAGGACGTG |
NGG |
2 |
0.5294 |
Tier III |
| 10 |
NC_000079.6 |
13 |
15814926 |
+ |
A530046M15Rik |
NNNGGTCATGCCAGGCCGTG |
NGG |
2 |
0.32 |
Tier III |
| 11 |
NC_000079.6 |
13 |
15814926 |
+ |
B230303A05Rik |
NNNGGTCATGCCAGGCCGTG |
NGG |
2 |
0.32 |
Tier III |
| 12 |
NC_000082.6 |
16 |
38992031 |
- |
Gm34258 |
NNNGGTCATGCCAGGCCGTG |
NGG |
2 |
0.32 |
Tier III |
| 13 |
NC_000086.7 |
X |
136749279 |
+ |
BC065397 |
NNNGTTCCTTCCAGGCCCTG |
NGG |
2 |
0.2041 |
Tier III |
Other clones with same target sequence:
(none)
