Construct: sgRNA BRDN0001147759
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCGAAGCTGACCCTACATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRM (6723)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76281
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
11056685 |
- |
SRM |
NNNGAAGCTGACCCTACATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
52646789 |
- |
KRT2 |
NNNGAAGCTGGCCCTAGATG |
NGG |
2 |
0.0382 |
Tier I |
| 3 |
NC_000004.12 |
4 |
68339275 |
+ |
YTHDC1 |
NNNAAAGCTGAACCTACATG |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000009.12 |
9 |
95902936 |
- |
ERCC6L2 |
NNNAAAGCTGACTCTACATG |
NGG |
2 |
0.63 |
Tier II |
| 5 |
NC_000003.12 |
3 |
85191883 |
- |
CADM2 |
NNNGTTGCTGACCCTACATG |
NGG |
2 |
0.2597 |
Tier II |
| 6 |
NC_000003.12 |
3 |
131846838 |
+ |
CPNE4 |
NNNTAAGCTGAACCTACATG |
NGG |
2 |
0.2597 |
Tier II |
| 7 |
NC_000011.10 |
11 |
895178 |
+ |
CHID1 |
NNNGAAGCTGACCCTACCTG |
NGG |
1 |
0.1905 |
Tier II |
| 8 |
NC_000010.11 |
10 |
7369771 |
+ |
SFMBT2 |
NNNCAAGCTGACCCTACCTG |
NGG |
2 |
0.1008 |
Tier II |
| 9 |
NC_000010.11 |
10 |
86329134 |
- |
GRID1 |
NNNGAAGCTGTCCCTGCATG |
NGG |
2 |
0.0592 |
Tier II |
| 10 |
NC_000003.12 |
3 |
40234232 |
+ |
MYRIP |
NNNGAAGCAGACCCTAGATG |
NGG |
2 |
0.0546 |
Tier II |
| 11 |
NC_000001.11 |
1 |
53685260 |
+ |
GLIS1 |
NNNGCAGCTGACCCTACATG |
NTG |
2 |
0.0195 |
Tier II |
| 12 |
NC_000009.12 |
9 |
925143 |
- |
DMRT1 |
NNNGAAGCTGACCCTCCGTG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000017.11 |
17 |
34851143 |
+ |
LOC105371742 |
NNNGCAGCTGACCCAACATG |
NGG |
2 |
0.2895 |
Tier III |
| 14 |
NC_000003.12 |
3 |
131846838 |
+ |
LOC105374113 |
NNNTAAGCTGAACCTACATG |
NGG |
2 |
0.2597 |
Tier III |
| 15 |
NC_000001.11 |
1 |
175287938 |
- |
LOC105371623 |
NNNGAAGCTGACACCACATG |
NGG |
2 |
0.1049 |
Tier III |
| 16 |
NC_000003.12 |
3 |
40234232 |
+ |
EIF1B-AS1 |
NNNGAAGCAGACCCTAGATG |
NGG |
2 |
0.0546 |
Tier III |
| 17 |
NC_000011.10 |
11 |
134761703 |
+ |
LINC02714 |
NNNGAAGCTGACCCGACAAG |
NGG |
2 |
0.0143 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
5018067 |
- |
Zfp524 |
NNNGAAGCGCACCCTACATG |
NGG |
2 |
0.2476 |
Tier I |
| 2 |
NC_000075.6 |
9 |
110085136 |
+ |
Dhx30 |
NNNGAAGCTGACCCTAGATG |
NAG |
2 |
0.0153 |
Tier I |
| 3 |
NC_000081.6 |
15 |
97858837 |
- |
Vdr |
NNNGAAGCTGAACCTCCATG |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000077.6 |
11 |
55418821 |
- |
Sparc |
NNNGATGCGGACCCTACATG |
NGG |
2 |
0.4422 |
Tier II |
| 5 |
NC_000070.6 |
4 |
141492367 |
+ |
Spen |
NNNGAAGGTGACCCTAAATG |
NGG |
2 |
0.3 |
Tier II |
| 6 |
NC_000070.6 |
4 |
87009129 |
+ |
Slc24a2 |
NNNGAGGCTCACCCTACATG |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000075.6 |
9 |
102310621 |
+ |
Ephb1 |
NNNGAAGCTGACCATACTTG |
NGG |
2 |
0.175 |
Tier II |
| 8 |
NC_000086.7 |
X |
93686850 |
+ |
Pcyt1b |
NNNGAAGCTGACCCTGCTTG |
NGG |
2 |
0.0962 |
Tier II |
| 9 |
NC_000080.6 |
14 |
55629232 |
+ |
Ipo4 |
NNNGAAGCTGACCCTAACTG |
NGG |
2 |
0.0889 |
Tier II |
| 10 |
NC_000073.6 |
7 |
56262860 |
+ |
Oca2 |
NNNGAAGCTGACCCAACATG |
NGA |
2 |
0.0402 |
Tier II |
| 11 |
NC_000077.6 |
11 |
65179068 |
+ |
Myocd |
NNNGAAACTGACCCTACATG |
NTG |
2 |
0.039 |
Tier II |
| 12 |
NC_000080.6 |
14 |
49188480 |
+ |
Naa30 |
NNNGAAGCTGACCCTACTTG |
NGA |
2 |
0.0347 |
Tier II |
| 13 |
NC_000067.6 |
1 |
177838440 |
+ |
Catspere1 |
NNNGAAGCTGACCCTACTTG |
NGT |
2 |
0.0081 |
Tier II |
| 14 |
NC_000067.6 |
1 |
178029749 |
+ |
Catspere2 |
NNNGAAGCTGACCCTACTTG |
NGT |
2 |
0.0081 |
Tier II |
| 15 |
NC_000068.7 |
2 |
163748394 |
+ |
Ada |
NNNGAAGCTGACCCTCAATG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000069.6 |
3 |
152592214 |
+ |
Ak5 |
NNNGAAGCTGACCCTTCATG |
NGG |
1 |
0.0 |
Tier II |
| 17 |
NC_000081.6 |
15 |
97858837 |
- |
Gm33690 |
NNNGAAGCTGAACCTCCATG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
