Construct: sgRNA BRDN0001147776
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGACGATGCCATTCTCATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK1G (57172)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75985
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
209605644 |
- |
CAMK1G |
NNNCGATGCCATTCTCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
30121887 |
+ |
MAPK3 |
NNNCGATGACATTCTCATGG |
NGG |
2 |
0.1513 |
Tier I |
| 3 |
NC_000003.12 |
3 |
85181367 |
+ |
CADM2 |
NNNAGATGCCATCCTCATGT |
NGG |
2 |
0.6648 |
Tier II |
| 4 |
NC_000018.10 |
18 |
62820546 |
+ |
PHLPP1 |
NNNCGATGCCATTCTCATGA |
NGG |
1 |
0.5625 |
Tier II |
| 5 |
NC_000011.10 |
11 |
89553297 |
+ |
NOX4 |
NNNCCATGCCATTCTCATGA |
NGG |
2 |
0.442 |
Tier II |
| 6 |
NC_000004.12 |
4 |
55441126 |
+ |
CLOCK |
NNNCGATGCCATACTCATCT |
NGG |
2 |
0.3103 |
Tier II |
| 7 |
NC_000004.12 |
4 |
55441126 |
+ |
TMEM165 |
NNNCGATGCCATACTCATCT |
NGG |
2 |
0.3103 |
Tier II |
| 8 |
NC_000005.10 |
5 |
144446056 |
+ |
KCTD16 |
NNNAGATGCCATTATCATGT |
NGG |
2 |
0.2947 |
Tier II |
| 9 |
NC_000011.10 |
11 |
7389849 |
- |
SYT9 |
NNNTGATGCCATTCTTATGT |
NGG |
2 |
0.2462 |
Tier II |
| 10 |
NC_000002.12 |
2 |
133325792 |
- |
NCKAP5 |
NNNAGATGCCATTCTCATGT |
NAG |
2 |
0.2183 |
Tier II |
| 11 |
NC_000010.11 |
10 |
85952140 |
- |
GRID1 |
NNNGGATGCCATTCCCATGT |
NGG |
2 |
0.1364 |
Tier II |
| 12 |
NC_000012.12 |
12 |
123051020 |
+ |
PITPNM2 |
NNNCGATGCCATTCTCATGT |
NCT |
2 |
0.0 |
Tier II |
| 13 |
NC_000006.12 |
6 |
111516418 |
- |
TRAF3IP2-AS1 |
NNNTGATGCCATTCTCAGGT |
NGG |
2 |
0.2667 |
Tier III |
| 14 |
NC_000015.10 |
15 |
87521212 |
+ |
LOC102724465 |
NNNCAATGCCATTGTCATGT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
193354987 |
+ |
Camk1g |
NNNCGATGCCATTCTCATGA |
NGG |
1 |
0.5625 |
Tier I |
| 2 |
NC_000078.6 |
12 |
4198388 |
- |
Adcy3 |
NNNCGATGCCCTTCTCATCT |
NGG |
2 |
0.1793 |
Tier I |
| 3 |
NC_000079.6 |
13 |
49821052 |
- |
Gm272 |
NNNCGATGCCATTCTCATGG |
NAG |
2 |
0.0458 |
Tier I |
| 4 |
NC_000080.6 |
14 |
7942073 |
- |
Flnb |
NNNGGATGCCATTCTCATGC |
NGG |
2 |
0.0455 |
Tier I |
| 5 |
NC_000080.6 |
14 |
31150988 |
- |
Stab1 |
NNNCAATGCCCTTCTCATGT |
NGG |
2 |
0.3467 |
Tier II |
| 6 |
NC_000078.6 |
12 |
89148845 |
- |
Nrxn3 |
NNNCGATGCCATTCTCAGAT |
NGG |
2 |
0.2381 |
Tier II |
| 7 |
NC_000078.6 |
12 |
4198388 |
- |
Cenpo |
NNNCGATGCCCTTCTCATCT |
NGG |
2 |
0.1793 |
Tier II |
| 8 |
NC_000070.6 |
4 |
134403395 |
+ |
Pafah2 |
NNNCGATGCCATCCTGATGT |
NGG |
2 |
0.1215 |
Tier II |
| 9 |
NC_000086.7 |
X |
95799915 |
- |
Zc3h12b |
NNNCAATGCCATTCTCATGT |
NTG |
2 |
0.0338 |
Tier II |
| 10 |
NC_000075.6 |
9 |
55152936 |
- |
Ube2q2 |
NNNCGATGCCATTCACATGT |
NGC |
2 |
0.0129 |
Tier II |
Other clones with same target sequence:
(none)
