Construct: sgRNA BRDN0001147808
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATCTCATACATCTTGTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MYO3B (140469)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75571
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
170214470 |
+ |
MYO3B |
NNNCTCATACATCTTGTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
126464761 |
+ |
C10orf90 |
NNNCACATACATCTTGTAAG |
NGG |
2 |
0.2143 |
Tier I |
| 3 |
NC_000005.10 |
5 |
94443474 |
+ |
KIAA0825 |
NNNCTCATAAATCTTGTACT |
NGG |
2 |
0.6067 |
Tier II |
| 4 |
NC_000006.12 |
6 |
162367693 |
- |
PRKN |
NNNCTAATACATCTTGTATG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000023.11 |
X |
111931760 |
- |
TRPC5 |
NNNCTCATACATCTAGTAAG |
NGG |
2 |
0.2481 |
Tier II |
| 6 |
NC_000006.12 |
6 |
65534031 |
+ |
EYS |
NNNCTCATACTTCTTGTACT |
NGG |
2 |
0.2154 |
Tier II |
| 7 |
NC_000006.12 |
6 |
162367693 |
- |
LOC105369171 |
NNNCTAATACATCTTGTATG |
NGG |
2 |
0.4286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
59629261 |
- |
E130308A19Rik |
NNNCTCATACATCTGGTACC |
NGG |
2 |
0.0214 |
Tier II |
| 2 |
NC_000072.6 |
6 |
138059767 |
- |
Slc15a5 |
NNNCTCATACATCTTGTACC |
NGT |
2 |
0.0069 |
Tier II |
Other clones with same target sequence:
(none)
