Construct: sgRNA BRDN0001147821

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAGGTGTGGCAAAACATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM7 (54822)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76112

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 50639536 - TRPM7 NNNGGTGTGGCAAAACATGT NGG 0 1.0 Tier I
2 NC_000005.10 5 137307433 - SPOCK1 NNNGGTGTGGCAGAACATGT NGG 1 0.6522 Tier II
3 NC_000018.10 18 61388663 + CDH20 NNNGGTGTGGAAAAACATTT NGG 2 0.5 Tier II
4 NC_000019.10 19 15619225 + CYP4F8 NNNGGTGTTGCAGAACATGT NGG 2 0.3478 Tier II
5 NC_000019.10 19 15927735 - CYP4F11 NNNGGTGTTGCAGAACATGT NGG 2 0.3478 Tier II
6 NC_000016.10 16 4519269 + CDIP1 NNNTCTGTGGCAAAACATGT NGG 2 0.2857 Tier II
7 NC_000009.12 9 132423856 - CFAP77 NNNAGTGTGGCATAACATGT NGG 2 0.27 Tier II
8 NC_000003.12 3 58409463 + PXK NNNGGTTTGCCAAAACATGT NGG 2 0.2286 Tier II
9 NC_000012.12 12 2228179 - CACNA1C NNNGGTGTGGGAAAACATCT NGG 2 0.1121 Tier II
10 NC_000006.12 6 13055315 + PHACTR1 NNNGGTGTCGCAAAACCTGT NGG 2 0.095 Tier II
11 NC_000022.11 22 25221734 - CRYBB2 NNNGGTGTGGCAAAACAGGT NAG 2 0.0864 Tier II
12 NC_000015.10 15 90054417 + ZNF710 NNNGGTGTGGGAAAACAGGT NGG 2 0.0833 Tier II
13 NC_000016.10 16 3793255 - CREBBP NNNGGTGTGGCAAGACATGT NGC 2 0.0104 Tier II
14 NC_000006.12 6 165468999 + PDE10A NNNGGTGTGGCAAAACAGGT NGT 2 0.0054 Tier II
15 NC_000003.12 3 971577 + LOC107986059 NNNGCTGTGGCAATACATGT NGG 2 0.419 Tier III
16 NC_000019.10 19 15573326 + CYP4F23P NNNGGTGTTGCAGAACATGT NGG 2 0.3478 Tier III
17 NC_000008.11 8 116300158 - LINC00536 NNNGGTGTGGCAAAACATCA NGG 2 0.2522 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 126848609 - Trpm7 NNNGGTGTGGCAAAACATGT NGG 0 1.0 Tier I
2 NC_000068.7 2 68421672 - Stk39 NNNGATGAGGCAAAACATGT NGG 2 0.6933 Tier II
3 NC_000071.6 5 62651193 + Arap2 NNNGATGTGGCAAAACATAT NGG 2 0.619 Tier II
4 NC_000074.6 8 47319997 - Stox2 NNNGGTGTGGCAGAACATGA NGG 2 0.3668 Tier II
5 NC_000072.6 6 96612240 + Tafa1 NNNGCTGTGGCAAAACATCT NGG 2 0.3522 Tier II
6 NC_000071.6 5 128739973 - Piwil1 NNNGGTGGGGCAAGACATGT NGG 2 0.3422 Tier II
7 NC_000073.6 7 66399434 + Aldh1a3 NNNGGTGTTGCAAAACACGT NGG 2 0.2286 Tier II
8 NC_000085.6 19 43474034 - Cnnm1 NNNGGTGTGGCATAGCATGT NGG 2 0.195 Tier II
9 NC_000084.6 18 36964686 + Pcdha4 NNNGTTTTGGCAAAACATGT NGG 2 0.1714 Tier II
10 NC_000084.6 18 36964686 + Pcdha5 NNNGTTTTGGCAAAACATGT NGG 2 0.1714 Tier II
11 NC_000084.6 18 36964686 + Pcdha1 NNNGTTTTGGCAAAACATGT NGG 2 0.1714 Tier II
12 NC_000084.6 18 36964686 + Pcdha3 NNNGTTTTGGCAAAACATGT NGG 2 0.1714 Tier II
13 NC_000084.6 18 36964686 + Pcdha2 NNNGTTTTGGCAAAACATGT NGG 2 0.1714 Tier II
14 NC_000084.6 18 36964686 + Gm37013 NNNGTTTTGGCAAAACATGT NGG 2 0.1714 Tier II
15 NC_000070.6 4 149294698 + Kif1b NNNGGTGTGGCAACACATGT NTG 2 0.0083 Tier II
16 NC_000072.6 6 50115238 + Mpp6 NNNGGTGTGGCAACACATGT NTG 2 0.0083 Tier II
17 NC_000074.6 8 117165110 + Gan NNNGGTGTGGCAACACATGT NTG 2 0.0083 Tier II
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Other clones with same target sequence:

(none)