Construct: sgRNA BRDN0001147837
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCCGTGCAGCGAGTCAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKZ (8525)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77464
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
46367367 |
+ |
DGKZ |
NNNCGTGCAGCGAGTCAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
36805240 |
- |
MTCL2 |
NNNCCTGCAGCGGGTCAGAG |
NGG |
2 |
0.5124 |
Tier II |
| 3 |
NC_000010.11 |
10 |
29479228 |
- |
SVIL |
NNNCGGGCAGAGAGTCAGAG |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000012.12 |
12 |
1820487 |
- |
CACNA2D4 |
NNNCGAGCAGCGAGCCAGAG |
NGG |
2 |
0.2364 |
Tier II |
| 5 |
NC_000017.11 |
17 |
81400747 |
- |
BAHCC1 |
NNNCGGGCAGGGAGTCAGAG |
NGG |
2 |
0.1429 |
Tier II |
| 6 |
NC_000011.10 |
11 |
6635777 |
- |
DCHS1 |
NNNCCTGCAGCGAGTCAGAG |
NGC |
2 |
0.0175 |
Tier II |
| 7 |
NC_000020.11 |
20 |
5500528 |
- |
LINC00654 |
NNNTGAGCAGCGAGTCAGAG |
NGG |
2 |
0.6933 |
Tier III |
| 8 |
NC_000020.11 |
20 |
5500528 |
- |
LOC124904863 |
NNNTGAGCAGCGAGTCAGAG |
NGG |
2 |
0.6933 |
Tier III |
| 9 |
NC_000013.11 |
13 |
43968661 |
+ |
DGKZP1 |
NNNGGTGCAGCGAGTCAGAG |
NGG |
1 |
0.5 |
Tier III |
| 10 |
NC_000010.11 |
10 |
29479228 |
- |
SVIL-AS1 |
NNNCGGGCAGAGAGTCAGAG |
NGG |
2 |
0.4286 |
Tier III |
| 11 |
NC_000010.11 |
10 |
30706016 |
+ |
SVIL2P |
NNNCATGCAGGGAGTCAGAG |
NGG |
2 |
0.2167 |
Tier III |
| 12 |
NC_000017.11 |
17 |
81400747 |
- |
MIR4740 |
NNNCGGGCAGGGAGTCAGAG |
NGG |
2 |
0.1429 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
4924280 |
+ |
Ppp1r9a |
NNNCATGCAGAGAGTCAGAG |
NGG |
2 |
0.65 |
Tier II |
| 2 |
NC_000073.6 |
7 |
84309527 |
- |
Arnt2 |
NNNCATGCAGCCAGTCAGAG |
NGG |
2 |
0.4588 |
Tier II |
| 3 |
NC_000084.6 |
18 |
61032876 |
+ |
Cdx1 |
NNNCGTGCAGAGAGTCAGAG |
NGA |
2 |
0.0521 |
Tier II |
| 4 |
NC_000077.6 |
11 |
95667459 |
- |
Phb |
NNNGGTGCAGCGAGGCAGAG |
NGG |
2 |
0.025 |
Tier II |
Other clones with same target sequence:
(none)
