Construct: sgRNA BRDN0001147841
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGCACCATAGAAACGTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AKT3 (10000)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76220
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
243573002 |
+ |
AKT3 |
NNNCACCATAGAAACGTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
158434992 |
- |
TULP4 |
NNNCAGCAGAGAAACGTGTG |
NGG |
2 |
0.3095 |
Tier II |
| 3 |
NC_000002.12 |
2 |
54642883 |
+ |
SPTBN1 |
NNNCACCATGGAAACGTGTG |
NAG |
2 |
0.144 |
Tier II |
| 4 |
NC_000018.10 |
18 |
49449449 |
- |
DYM |
NNNCACCATAGAAAAGTCTG |
NGG |
2 |
0.1058 |
Tier II |
| 5 |
NC_000017.11 |
17 |
38909270 |
- |
LASP1 |
NNNAACCATAGAAAGGTGTG |
NGG |
2 |
0.0421 |
Tier II |
| 6 |
NC_000014.9 |
14 |
80187014 |
- |
LOC105370593 |
NNNCACTTTAGAAACGTGTG |
NGG |
2 |
0.3482 |
Tier III |
| 7 |
NC_000008.11 |
8 |
141456334 |
+ |
MROH5 |
NNNCACAATAGAAACGTGTG |
NAG |
2 |
0.1944 |
Tier III |
| 8 |
NC_000008.11 |
8 |
141456334 |
+ |
LOC105375789 |
NNNCACAATAGAAACGTGTG |
NAG |
2 |
0.1944 |
Tier III |
| 9 |
NC_000010.11 |
10 |
57790725 |
- |
LOC105378314 |
NNNCACCATAGAAAAGTCTG |
NGG |
2 |
0.1058 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
177067287 |
+ |
Akt3 |
NNNCACCATAGAAACGTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
69475537 |
+ |
Dnah2 |
NNNCAAAATAGAAACGTGTG |
NGG |
2 |
0.6964 |
Tier I |
| 3 |
NC_000085.6 |
19 |
3918746 |
+ |
Aldh3b1 |
NNNCTCCATAGAAACGTGTG |
NTG |
2 |
0.0142 |
Tier I |
| 4 |
NC_000068.7 |
2 |
181255908 |
- |
Gmeb2 |
NNNCACCATAGAGACCTGTG |
NGG |
2 |
0.0 |
Tier I |
| 5 |
NC_000086.7 |
X |
159495952 |
+ |
Map7d2 |
NNNAACCATAGAAAAGTGTG |
NGG |
2 |
0.1871 |
Tier II |
| 6 |
NC_000071.6 |
5 |
73189974 |
+ |
Fryl |
NNNAACCATAGAAACGGGTG |
NGG |
2 |
0.0991 |
Tier II |
| 7 |
NC_000078.6 |
12 |
69883119 |
- |
Map4k5 |
NNNCCCCATAGAAATGTGTG |
NGG |
2 |
0.0333 |
Tier II |
| 8 |
NC_000072.6 |
6 |
53624491 |
+ |
Creb5 |
NNNCACCATAGAAACGTCTG |
NGC |
2 |
0.0106 |
Tier II |
| 9 |
NC_000076.6 |
10 |
40871543 |
+ |
Cdc40 |
NNNCACCATAGAAACTGGTG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000077.6 |
11 |
60414654 |
- |
Atpaf2 |
NNNCACCATAGAAACTTGTC |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000073.6 |
7 |
35096611 |
+ |
Gm35611 |
NNNCACCATAGACACATGTG |
NGG |
2 |
0.2105 |
Tier III |
| 12 |
NC_000085.6 |
19 |
3918746 |
+ |
Gm42059 |
NNNCTCCATAGAAACGTGTG |
NTG |
2 |
0.0142 |
Tier III |
| 13 |
NC_000076.6 |
10 |
18804680 |
+ |
4930444F02Rik |
NNNCACCATAGACACGTGTG |
NTG |
2 |
0.0082 |
Tier III |
| 14 |
NC_000076.6 |
10 |
18804680 |
+ |
Gm32926 |
NNNCACCATAGACACGTGTG |
NTG |
2 |
0.0082 |
Tier III |
| 15 |
NC_000086.7 |
X |
19183608 |
- |
Gm14636 |
NNNCACCATAGAAACTTGGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
