Construct: sgRNA BRDN0001147847
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAACTCCTTCATTTCGGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IP6K1 (9807)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76135
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
49747850 |
+ |
IP6K1 |
NNNACTCCTTCATTTCGGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
26222168 |
- |
ANO3 |
NNNAATCCTTCATTTTGGGA |
NGG |
2 |
0.1758 |
Tier II |
| 3 |
NC_000011.10 |
11 |
72238794 |
- |
INPPL1 |
NNNATTCCTTCATTTCCGGA |
NGG |
2 |
0.1497 |
Tier II |
| 4 |
NC_000019.10 |
19 |
41298597 |
- |
HNRNPUL1 |
NNNACTCCTTCGTTTGGGGA |
NGG |
2 |
0.1111 |
Tier II |
| 5 |
NC_000002.12 |
2 |
168607842 |
- |
CERS6 |
NNNACTCCTTCATATGGGGA |
NGG |
2 |
0.0952 |
Tier II |
| 6 |
NC_000005.10 |
5 |
132748828 |
- |
CCNI2 |
NNNATTCCTTCATTTCGGGA |
NTG |
2 |
0.0248 |
Tier II |
| 7 |
NC_000011.10 |
11 |
69073224 |
- |
TPCN2 |
NNNACTCCTTCATGTCGGGC |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
108024419 |
- |
Ip6k1 |
NNNATTCCTTCATTTCGGGA |
NGG |
1 |
0.6364 |
Tier I |
| 2 |
NC_000077.6 |
11 |
53969517 |
- |
Slc22a21 |
NNNGCTCCTTCATTTCGGGA |
NAG |
2 |
0.162 |
Tier I |
| 3 |
NC_000068.7 |
2 |
141457334 |
- |
Macrod2 |
NNNAATCCTTCATTTAGGGA |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000070.6 |
4 |
97886008 |
- |
Nfia |
NNNACTCCTTCATTTCGACA |
NGG |
2 |
0.3103 |
Tier II |
| 5 |
NC_000078.6 |
12 |
53337221 |
- |
Npas3 |
NNNCCTCCTTCACTTCGGGA |
NGG |
2 |
0.2786 |
Tier II |
| 6 |
NC_000075.6 |
9 |
49056100 |
+ |
Zw10 |
NNNACTCCTTCATTTATGGA |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000067.6 |
1 |
8888822 |
- |
Sntg1 |
NNNACTCCTTCATTTCTGGT |
NGG |
2 |
0.15 |
Tier II |
| 8 |
NC_000068.7 |
2 |
21652470 |
- |
Gpr158 |
NNNACTCCTTCATTTCTGGT |
NGG |
2 |
0.15 |
Tier II |
| 9 |
NC_000071.6 |
5 |
19772914 |
+ |
Magi2 |
NNNACTCCTTCATTTCTGGT |
NGG |
2 |
0.15 |
Tier II |
| 10 |
NC_000075.6 |
9 |
3723494 |
- |
Gucy1a2 |
NNNACTCCTTCATTTCTGGT |
NGG |
2 |
0.15 |
Tier II |
| 11 |
NC_000079.6 |
13 |
70809365 |
- |
Adamts16 |
NNNACTCCTTCATTTCTGGT |
NGG |
2 |
0.15 |
Tier II |
| 12 |
NC_000080.6 |
14 |
16931995 |
+ |
Rarb |
NNNACTCCTTCATTTCTGGT |
NGG |
2 |
0.15 |
Tier II |
| 13 |
NC_000070.6 |
4 |
20598161 |
- |
Nkain3 |
NNNACTCCTCCATTTCTGGA |
NGG |
2 |
0.1333 |
Tier II |
| 14 |
NC_000075.6 |
9 |
49734676 |
+ |
Ncam1 |
NNNCCTCCTTCATTTCTGGA |
NGG |
2 |
0.0882 |
Tier II |
| 15 |
NC_000084.6 |
18 |
70100523 |
+ |
Rab27b |
NNNACTCCTTCATTTCGGAA |
NGA |
2 |
0.0496 |
Tier II |
| 16 |
NC_000070.6 |
4 |
104341615 |
- |
Dab1 |
NNNACTCCGTCATTGCGGGA |
NGG |
2 |
0.031 |
Tier II |
| 17 |
NC_000078.6 |
12 |
106529578 |
- |
Gm32935 |
NNNACTCATTCATTTCGGGA |
NGG |
1 |
0.65 |
Tier III |
| 18 |
NC_000081.6 |
15 |
36629853 |
+ |
Gm6704 |
NNNACTTCTTCAGTTCGGGA |
NGG |
2 |
0.212 |
Tier III |
| 19 |
NC_000071.6 |
5 |
19772914 |
+ |
Gm52765 |
NNNACTCCTTCATTTCTGGT |
NGG |
2 |
0.15 |
Tier III |
| 20 |
NC_000084.6 |
18 |
70100523 |
+ |
Gm41770 |
NNNACTCCTTCATTTCGGAA |
NGA |
2 |
0.0496 |
Tier III |
| 21 |
NC_000069.6 |
3 |
73143748 |
- |
Gm20754 |
NNNACTCCTCCATTGCGGGA |
NGG |
2 |
0.0267 |
Tier III |
Other clones with same target sequence:
(none)
