Construct: sgRNA BRDN0001147852
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAACGTGATATCTTGGTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA3 (6197)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75944
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
20195104 |
- |
RPS6KA3 |
NNNACGTGATATCTTGGTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
120645999 |
- |
KCND2 |
NNNAAGTCATATCTTGGTAG |
NGG |
2 |
0.3516 |
Tier II |
| 3 |
NC_000007.14 |
7 |
78094092 |
- |
MAGI2 |
NNNACTTGATATCTTGGTAC |
NGG |
2 |
0.2857 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
159326883 |
+ |
Rps6ka3 |
NNNACGTGATATCTTGGTAG |
NAG |
1 |
0.2593 |
Tier I |
| 2 |
NC_000081.6 |
15 |
59014456 |
- |
Mtss1 |
NNNCCGTGATATCTTGATAG |
NGG |
2 |
0.3294 |
Tier II |
| 3 |
NC_000085.6 |
19 |
61107503 |
+ |
Zfp950 |
NNNACGTGCTTTCTTGGTAG |
NGG |
2 |
0.1758 |
Tier II |
| 4 |
NC_000069.6 |
3 |
31918230 |
+ |
Kcnmb2 |
NNNACGTGATATGTTGGTAA |
NGG |
2 |
0.1278 |
Tier II |
| 5 |
NC_000084.6 |
18 |
47659392 |
+ |
Gm5095 |
NNNACGTGCTATCTTGGTAC |
NGG |
2 |
0.2449 |
Tier III |
Other clones with same target sequence:
(none)
