Construct: sgRNA BRDN0001147860
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGCATCTCACGATACCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AK9 (221264)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77692
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
109573506 |
- |
AK9 |
NNNCATCTCACGATACCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
93455966 |
- |
FNBP1L |
NNNCATCTCACAATGCCCGA |
NGG |
2 |
0.6067 |
Tier II |
| 3 |
NC_000022.11 |
22 |
24624221 |
- |
GGT1 |
NNNCATCTCACCATGCCCGA |
NGG |
2 |
0.3441 |
Tier II |
| 4 |
NC_000015.10 |
15 |
31132827 |
+ |
TRPM1 |
NNNCATCTCACGAGACCTGA |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000013.11 |
13 |
18258179 |
+ |
LOC101060145 |
NNNCATCTCACCATGCCCGA |
NGG |
2 |
0.3441 |
Tier III |
| 6 |
NC_000022.11 |
22 |
18341281 |
- |
GGTLC5P |
NNNCATCTCACCATGCCCGA |
NGG |
2 |
0.3441 |
Tier III |
| 7 |
NC_000022.11 |
22 |
18778426 |
+ |
GGT3P |
NNNCATCTCACCATGCCCGA |
NGG |
2 |
0.3441 |
Tier III |
| 8 |
NC_000022.11 |
22 |
21212761 |
+ |
GGT2P |
NNNCATCTCACCATGCCCGA |
NGG |
2 |
0.3441 |
Tier III |
| 9 |
NC_000005.10 |
5 |
139712714 |
+ |
LOC124901085 |
NNNCACCTCACGAGACCCGA |
NGG |
2 |
0.0 |
Tier III |
| 10 |
NC_000021.9 |
21 |
25389840 |
+ |
LINC00158 |
NNNCATCTCACGAGATCCGA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
48365599 |
+ |
Sorcs3 |
NNNCATCTCCAGATACCCGA |
NGG |
2 |
0.25 |
Tier II |
| 2 |
NC_000086.7 |
X |
36436175 |
- |
Gm14569 |
NNNCATCTCACCATACCCCA |
NGG |
2 |
0.2373 |
Tier II |
Other clones with same target sequence:
(none)
