Construct: sgRNA BRDN0001147862
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGCATGACTTACCTCTAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FPGT-TNNI3K (100526835)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75903
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
74271705 |
- |
TNNI3K |
NNNCATGACTTACCTCTAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
74271705 |
- |
FPGT-TNNI3K |
NNNCATGACTTACCTCTAGG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000020.11 |
20 |
33707777 |
- |
PXMP4 |
NNNCATGACCTACCTCTATG |
NGG |
2 |
0.3556 |
Tier I |
| 4 |
NC_000012.12 |
12 |
7318179 |
- |
ACSM4 |
NNNCATGACTTACGTCTAGG |
NAG |
2 |
0.0 |
Tier I |
| 5 |
NC_000018.10 |
18 |
49951124 |
+ |
MYO5B |
NNNCATGACTTGCTTCTAGG |
NGG |
2 |
0.5296 |
Tier II |
| 6 |
NC_000003.12 |
3 |
57122786 |
+ |
IL17RD |
NNNCATGGCTTTCCTCTAGG |
NGG |
2 |
0.2444 |
Tier II |
| 7 |
NC_000007.14 |
7 |
8592438 |
- |
NXPH1 |
NNNCATGACTTACCCCTAAG |
NGG |
2 |
0.1948 |
Tier II |
| 8 |
NC_000003.12 |
3 |
12011122 |
+ |
SYN2 |
NNNCATGACTTCACTCTAGG |
NGG |
2 |
0.1012 |
Tier II |
| 9 |
NC_000016.10 |
16 |
20719381 |
- |
ACSM3 |
NNNCATGACTTACATCTAGG |
NAG |
2 |
0.0907 |
Tier II |
| 10 |
NC_000023.11 |
X |
24212173 |
- |
ZFX |
NNNCATGAGTTACCTCTAGG |
NGA |
2 |
0.043 |
Tier II |
| 11 |
NC_000007.14 |
7 |
8592438 |
- |
LOC105375144 |
NNNCATGACTTACCCCTAAG |
NGG |
2 |
0.1948 |
Tier III |
| 12 |
NC_000023.11 |
X |
34387771 |
+ |
SRSF2P1 |
NNNCATGACTTCCCTCAAGG |
NGG |
2 |
0.1404 |
Tier III |
| 13 |
NC_000016.10 |
16 |
20719381 |
- |
LOC100887080 |
NNNCATGACTTACATCTAGG |
NAG |
2 |
0.0907 |
Tier III |
| 14 |
NC_000016.10 |
16 |
20719381 |
- |
LOC124903661 |
NNNCATGACTTACATCTAGG |
NAG |
2 |
0.0907 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
154587900 |
- |
Pxmp4 |
NNNCATGACCTACCTCTACG |
NGG |
2 |
0.2391 |
Tier I |
| 2 |
NC_000076.6 |
10 |
94139884 |
- |
Fgd6 |
NNNCATGACTTACCTCTGCG |
NGG |
2 |
0.1793 |
Tier I |
| 3 |
NC_000074.6 |
8 |
80613803 |
- |
Frem3 |
NNNCATCACTTACCTCTAGG |
NAG |
2 |
0.1782 |
Tier I |
| 4 |
NC_000072.6 |
6 |
141313698 |
- |
Pde3a |
NNNCATGACTTACCTCCAGT |
NGG |
2 |
0.4941 |
Tier II |
| 5 |
NC_000075.6 |
9 |
50495723 |
+ |
Plet1 |
NNNCATGACTTACATCTAAG |
NGG |
2 |
0.25 |
Tier II |
| 6 |
NC_000071.6 |
5 |
135682258 |
+ |
Por |
NNNCATGACTTCCCTCCAGG |
NGG |
2 |
0.1858 |
Tier II |
| 7 |
NC_000067.6 |
1 |
106369811 |
+ |
Phlpp1 |
NNNCATGACTTAGCACTAGG |
NGG |
2 |
0.0789 |
Tier II |
| 8 |
NC_000071.6 |
5 |
118751103 |
- |
Med13l |
NNNCATGACTTAGCTCTAGG |
NTG |
2 |
0.0053 |
Tier II |
| 9 |
NC_000079.6 |
13 |
71541724 |
- |
1700112M02Rik |
NNNCATGACTTACCTCTAAA |
NGG |
2 |
0.6696 |
Tier III |
| 10 |
NC_000075.6 |
9 |
50495723 |
+ |
Plet1os |
NNNCATGACTTACATCTAAG |
NGG |
2 |
0.25 |
Tier III |
Other clones with same target sequence:
(none)
