Construct: sgRNA BRDN0001147867

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATCCTAAGAAGAAATATACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK1 (5058)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76465

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 77353562 - PAK1 NNNCTAAGAAGAAATATACA NGG 0 1.0 Tier I
2 NC_000003.12 3 196810628 + PAK2 NNNCTAAGAAAAAATATACA NGA 2 0.0694 Tier I
3 NC_000003.12 3 2781072 + CNTN4 NNNATAAAAAGAAATATACA NGG 2 0.8421 Tier II
4 NC_000012.12 12 72619283 + TRHDE NNNTTAAGAAGAAATATACA NGG 1 0.8 Tier II
5 NC_000003.12 3 4674557 + ITPR1 NNNCTTAAAAGAAATATACA NGG 2 0.7143 Tier II
6 NC_000001.11 1 231397825 + EGLN1 NNNCTAAGGAGAAATATACA NGG 1 0.6667 Tier II
7 NC_000007.14 7 142955608 - KEL NNNCTAAGGTGAAATATACA NGG 2 0.5882 Tier II
8 NC_000012.12 12 15686650 + EPS8 NNNCTAAGAAGAAATAAACA NGG 1 0.5333 Tier II
9 NC_000002.12 2 184604896 + ZNF804A NNNATAATAAGAAATATACA NGG 2 0.5263 Tier II
10 NC_000018.10 18 62359078 - TNFRSF11A NNNTTAATAAGAAATATACA NGG 2 0.5 Tier II
11 NC_000007.14 7 3020259 + CARD11 NNNCTAAGGAGGAATATACA NGG 2 0.4815 Tier II
12 NC_000012.12 12 1165663 + ERC1 NNNTTAAGAAGAAATAAACA NGG 2 0.4267 Tier II
13 NC_000002.12 2 205521286 - PARD3B NNNAAAAGAAGAAATATACA NGG 2 0.4211 Tier II
14 NC_000005.10 5 108287491 + FBXL17 NNNAAAAGAAGAAATATACA NGG 2 0.4211 Tier II
15 NC_000010.11 10 115930170 - ATRNL1 NNNCTTAGAAGAAATAAACA NGG 2 0.381 Tier II
16 NC_000002.12 2 50422815 + NRXN1 NNNCATAGAAGAAATATACA NGG 2 0.3571 Tier II
17 NC_000018.10 18 74763231 - ZNF407 NNNCAAAGAAGAAATACACA NGG 2 0.3529 Tier II
18 NC_000018.10 18 62547870 - ZCCHC2 NNNCTAAGAAGAAAAAAACA NGG 2 0.3088 Tier II
19 NC_000015.10 15 60517987 - RORA NNNCTGAGAAGAAATATGCA NGG 2 0.2857 Tier II
20 NC_000023.11 X 24794287 - POLA1 NNNCTAAGAAGAATTAAACA NGG 2 0.2844 Tier II
21 NC_000008.11 8 68734125 + C8orf34 NNNCGAAGAAGAAATATAAA NGG 2 0.2743 Tier II
22 NC_000023.11 X 68365095 + OPHN1 NNNCAAAGAAGAAATAAACA NGG 2 0.2667 Tier II
23 NC_000003.12 3 126634066 + TXNRD3 NNNCTAAGAAGAAATAATCA NGG 2 0.2667 Tier II
24 NC_000001.11 1 220570431 - MARK1 NNNTTAAGAAGATATATACA NGG 2 0.24 Tier II
25 NC_000014.9 14 50719895 + NIN NNNCTAAGATGAAATATACA NAG 2 0.2288 Tier II
26 NC_000010.11 10 21148061 + NEBL NNNCTAAGAAGAACTATACA NGG 1 0.2143 Tier II
27 NC_000004.12 4 2323338 - ZFYVE28 NNNCTAAGAAGATATACACA NGG 2 0.2118 Tier II
28 NC_000013.11 13 37012932 + SUPT20H NNNCTAAGAAAACATATACA NGG 2 0.2105 Tier II
29 NC_000007.14 7 4846906 + RADIL NNNCAAAGAATAAATATACA NGG 2 0.1923 Tier II
30 NC_000008.11 8 100716865 - PABPC1 NNNCTAAGAAGAAATACACA NAG 2 0.183 Tier II
31 NC_000005.10 5 133743076 + FSTL4 NNNCTAGGAAGAAATATACC NGG 2 0.1604 Tier II
32 NC_000016.10 16 75391221 + CFDP1 NNNTTAAGAAGAAATATCCA NGG 2 0.1524 Tier II
33 NC_000006.12 6 156893651 + ARID1B NNNCAAAGAAGATATATACA NGG 2 0.15 Tier II
34 NC_000023.11 X 53606335 + HUWE1 NNNCAAAGAAGATATATACA NGG 2 0.15 Tier II
35 NC_000012.12 12 103260210 + C12orf42 NNNCTAAAAAGAAATAGACA NGG 2 0.1176 Tier II
36 NC_000008.11 8 1242637 - DLGAP2 NNNCTAAGAACAAACATACA NGG 2 0.1169 Tier II
37 NC_000001.11 1 50460038 - FAF1 NNNCTATGAAGAACTATACA NGG 2 0.0938 Tier II
38 NC_000001.11 1 208064728 - PLXNA2 NNNCTAAGAAGAAATACAGA NGG 2 0.0882 Tier II
39 NC_000013.11 13 43871230 - CCDC122 NNNCGAAGAAGAAATATAGA NGG 2 0.08 Tier II
40 NC_000004.12 4 152468784 + FBXW7 NNNCTAAGATGAAATATACA NGA 2 0.0613 Tier II
41 NC_000011.10 11 22275876 - ANO5 NNNCTAAGAAGAAATACACA NGA 2 0.049 Tier II
42 NC_000006.12 6 49852798 + CRISP1 NNNCTAAGAAGAAAAATACA NGA 2 0.0402 Tier II
43 NC_000009.12 9 106866153 + ZNF462 NNNCTAAGAAGAAATATAGA NAG 2 0.0324 Tier II
44 NC_000002.12 2 114578463 - DPP10 NNNCTAAGAAGAAATAGACA NAG 2 0.0305 Tier II
45 NC_000014.9 14 71352680 - SIPA1L1 NNNCTAAAAAGAAATATACA NGC 2 0.0222 Tier II
46 NC_000023.11 X 9756613 + GPR143 NNNCTAAGAAGATATATACA NGA 2 0.0208 Tier II
47 NC_000007.14 7 102023644 - CUX1 NNNCTAAGAAGAAAGATACA NAG 2 0.013 Tier II
48 NC_000010.11 10 16624884 + RSU1 NNNCTAAGAAGAAATATACC NTG 2 0.0089 Tier II
49 NC_000017.11 17 66760326 + PRKCA NNNCTAAGAAGAAATCTAAA NGG 2 0.0 Tier II
50 NC_000004.12 4 13506340 + LOC124900669 NNNCTAAGAAAAGATATACA NGG 2 0.6522 Tier III
51 NC_000006.12 6 90822950 - LOC107986623 NNNCTAAAAAGAATTATACA NGG 2 0.5333 Tier III
52 NC_000015.10 15 93170367 - LOC101927025 NNNCAAAAAAGAAATATACA NGG 2 0.5 Tier III
53 NC_000022.11 22 27956080 + TTC28-AS1 NNNTTAATAAGAAATATACA NGG 2 0.5 Tier III
54 NC_000018.10 18 56005032 - LINC03069 NNNCAAAGAAGAAATACACA NGG 2 0.3529 Tier III
55 NC_000018.10 18 56005032 - LINC03092 NNNCAAAGAAGAAATACACA NGG 2 0.3529 Tier III
56 NC_000001.11 1 229269229 - RAB4A-AS1 NNNGGAAGAAGAAATATACA NGG 2 0.32 Tier III
57 NC_000015.10 15 60517987 - RORA-AS1 NNNCTGAGAAGAAATATGCA NGG 2 0.2857 Tier III
58 NC_000006.12 6 30275902 + HCG17 NNNCTGAGAATAAATATACA NGG 2 0.2747 Tier III
59 NC_000020.11 20 58820267 + GNAS-AS1 NNNCTATCAAGAAATATACA NGG 2 0.2692 Tier III
60 NC_000008.11 8 64026607 - LINC01414 NNNCTAAGAAGAAATATTAA NGG 2 0.2143 Tier III
61 NC_000012.12 12 110096286 - LOC105369976 NNNCTAAGAAGATATACACA NGG 2 0.2118 Tier III
62 NC_000002.12 2 40892481 + LOC105374497 NNNCAAAGAAGATATATACA NGG 2 0.15 Tier III
63 NC_000009.12 9 115154305 - DELEC1 NNNCTAAGAAGAAATAAACA NAG 2 0.1383 Tier III
64 NC_000002.12 2 113692342 + LINC02936 NNNCTAAGAAGATATATAAA NGG 2 0.1286 Tier III
65 NC_000006.12 6 133562181 - TARID NNNCTAAGAAGATATATAAA NGG 2 0.1286 Tier III
66 NC_000003.12 3 78021781 - LOC105377171 NNNCTAAGAAGCAGTATACA NGG 2 0.1228 Tier III
67 NC_000004.12 4 177224080 - LOC105377557 NNNATAAGAAGAAATAGACA NGG 2 0.0991 Tier III
68 NC_000005.10 5 159602951 - LOC105377684 NNNCTAAGAAGAAATATAGG NGG 2 0.0956 Tier III
69 NC_000001.11 1 50460038 - FAF1-AS1 NNNCTATGAAGAACTATACA NGG 2 0.0938 Tier III
70 NC_000006.12 6 71287216 - LOC124901339 NNNCTAATAAGAAATAGACA NGG 2 0.0735 Tier III
71 NC_000005.10 5 88372681 + TMEM161B-DT NNNCTAAGAAGATATATAGA NGG 2 0.0375 Tier III
72 NC_000005.10 5 2049036 + LOC105374618 NNNCAAAGAAGAAATATACA NTG 2 0.0195 Tier III
73 NC_000004.12 4 171296345 + LINC02504 NNNCTAAGAAAAAATATACA NGT 2 0.0161 Tier III
74 NC_000001.11 1 238583484 + LOC124904565 NNNCTAAGAACAAATATACA NGC 2 0.0095 Tier III
75 NC_000007.14 7 9708813 - LOC105375148 NNNCTAAGAAGAAATATAGA NGA 2 0.0087 Tier III
76 NC_000007.14 7 84537683 - LINC03017 NNNCTATGAAGAAATATACA NGT 2 0.0071 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 58872156 + Ecpas NNNCCAAGAAGAAATATAAA NGG 2 0.4286 Tier I
2 NC_000073.6 7 97888995 + Pak1 NNNCTAAGAAGAAGTACACA NGG 2 0.3294 Tier I
3 NC_000077.6 11 69984787 - Ctdnep1 NNNCTAAGAAGAAATCCACA NGG 2 0.0 Tier I
4 NC_000074.6 8 68658038 + Csgalnact1 NNNCTGAGAAGGAATATACA NGG 2 0.5159 Tier II
5 NC_000067.6 1 191572702 + Dtl NNNCTCAAAAGAAATATACA NGG 2 0.4545 Tier II
6 NC_000068.7 2 119121933 - Rad51 NNNCTACAAAGAAATATACA NGG 2 0.4375 Tier II
7 NC_000078.6 12 116971819 - Ptprn2 NNNCTAAGAAGAATTACACA NGG 2 0.3765 Tier II
8 NC_000078.6 12 13329957 + Nbas NNNTTCAGAAGAAATATACA NGG 2 0.3636 Tier II
9 NC_000072.6 6 95606953 - Suclg2 NNNCAAGGAAGAAATATACA NGG 2 0.3529 Tier II
10 NC_000080.6 14 12717761 - Cadps NNNCTAAGAAATAATATACA NGG 2 0.3333 Tier II
11 NC_000081.6 15 28306981 + Dnah5 NNNCTATGAAGAAAAATACA NGG 2 0.2533 Tier II
12 NC_000086.7 X 165210446 - Glra2 NNNCTAAGAAGAAAAATAAA NGG 2 0.2481 Tier II
13 NC_000075.6 9 67881187 - Vps13c NNNCTAACAAGAAATATGCA NGG 2 0.2462 Tier II
14 NC_000069.6 3 55515729 - Dclk1 NNNCTAAGTAGAAATATGCA NGG 2 0.24 Tier II
15 NC_000081.6 15 21394965 - Cdh12 NNNCTAAGAAGAAATAAAAA NGG 2 0.2286 Tier II
16 NC_000067.6 1 64834716 + Plekhm3 NNNCAAAGAAGAAATATAAA NGG 2 0.2143 Tier II
17 NC_000068.7 2 124382614 + Sema6d NNNCTACGAAGAAGTATACA NGG 2 0.2042 Tier II
18 NC_000067.6 1 95645385 + St8sia4 NNNCTATGAAGAAGTATACA NGG 2 0.2042 Tier II
19 NC_000073.6 7 27734364 - Zfp60 NNNCTAGGAAGAAACATACA NGG 2 0.1925 Tier II
20 NC_000075.6 9 50920248 - Sik2 NNNCTATGAAGAAATATAAA NGG 2 0.1875 Tier II
21 NC_000076.6 10 36583529 - Hs3st5 NNNCTAAGAATAAATATGCA NGG 2 0.1538 Tier II
22 NC_000069.6 3 83142612 - Dchs2 NNNCTAAGAAGAAACATAAA NGG 2 0.1169 Tier II
23 NC_000073.6 7 89833195 - Me3 NNNCTAAGAAGACATAAACA NGG 2 0.1123 Tier II
24 NC_000067.6 1 56980263 + Satb2 NNNCTAAGAAGAAAAGTACA NGG 2 0.1113 Tier II
25 NC_000076.6 10 95176405 + Cradd NNNCTAAGAAGAAAAATCCA NGG 2 0.1103 Tier II
26 NC_000078.6 12 28542035 + Dcdc2c NNNCTAAGAATAAATATACA NAG 2 0.0997 Tier II
27 NC_000075.6 9 80231210 - Myo6 NNNCTAAGAAGAAATAGAAA NGG 2 0.0504 Tier II
28 NC_000085.6 19 4392415 + Kdm2a NNNCTAAGAAGAAATATACT NGA 2 0.0417 Tier II
29 NC_000076.6 10 121301704 + Tbc1d30 NNNCTATGAAGAAATATACA NGA 2 0.0304 Tier II
30 NC_000074.6 8 86707677 - Lonp2 NNNCTAATAAGAAATATACA NTG 2 0.0244 Tier II
31 NC_000068.7 2 158483521 - Ralgapb NNNCTAATAAGAAATATACA NGC 2 0.0139 Tier II
32 NC_000084.6 18 60620378 + Synpo NNNCAAAGAAGAAATTTACA NGG 2 0.0 Tier II
33 NC_000069.6 3 138282130 - Adh1 NNNCTAAGAAGAAATATACA NTT 2 0.0 Tier II
34 NC_000080.6 14 39298443 - Nrg3 NNNCTAAGAAGAAATCCACA NGG 2 0.0 Tier II
35 NC_000067.6 1 174223596 + Spta1 NNNCTAAGAAGAAATTTACT NGG 2 0.0 Tier II
36 NC_000083.6 17 9533703 + Gm51424 NNNATAAAAAGAAATATACA NGG 2 0.8421 Tier III
37 NC_000074.6 8 67226606 + Gm32623 NNNCTAAAAAGAAATAAACA NGG 2 0.5333 Tier III
38 NC_000083.6 17 16371734 + Gm41534 NNNCTAAGATGAAATATACA NAG 2 0.2288 Tier III
39 NC_000086.7 X 81347300 - Gm52423 NNNCTGAGAAGATATATACA NGG 2 0.2143 Tier III
40 NC_000071.6 5 97487095 + 4930467D21Rik NNNCTAAGATGAAATATCCA NGG 2 0.1681 Tier III
41 NC_000067.6 1 177557410 + Gm30013 NNNCTAAGAAGAAATACACA NGA 2 0.049 Tier III
42 NC_000083.6 17 7940276 + Gm1604a NNNCTAAGAAGAAATATATA NGA 2 0.0321 Tier III
43 NC_000077.6 11 69984787 - Gm51904 NNNCTAAGAAGAAATCCACA NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)