Construct: sgRNA BRDN0001147874
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACAGGGTGATGGACCATCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ARSG (22901)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77641
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
68351682 |
+ |
ARSG |
NNNAGGGTGATGGACCATCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
133100408 |
+ |
RALGDS |
NNNCGGGGGATGGACCATCA |
NGG |
2 |
0.2588 |
Tier II |
| 3 |
NC_000007.14 |
7 |
3927609 |
+ |
SDK1 |
NNNAGGGTGATGGTCCACCA |
NGG |
2 |
0.2286 |
Tier II |
| 4 |
NC_000011.10 |
11 |
70891786 |
- |
SHANK2 |
NNNAGGGTGAGGGTCCATCA |
NGG |
2 |
0.2133 |
Tier II |
| 5 |
NC_000006.12 |
6 |
12240946 |
+ |
EDN1 |
NNNAGGGAGATGGAACATCA |
NGG |
2 |
0.1778 |
Tier II |
| 6 |
NC_000017.11 |
17 |
61832921 |
+ |
BRIP1 |
NNNAGGGTGAGGGAACATCA |
NGG |
2 |
0.0889 |
Tier II |
| 7 |
NC_000020.11 |
20 |
50845438 |
- |
BCAS4 |
NNNAGGGTGATGGCCCTTCA |
NGG |
2 |
0.0286 |
Tier II |
| 8 |
NC_000002.12 |
2 |
190951212 |
+ |
GLS |
NNNAGGGTGATGGAGCAGCA |
NGG |
2 |
0.0167 |
Tier II |
| 9 |
NC_000007.14 |
7 |
18410238 |
+ |
HDAC9 |
NNNAGGGTGATGGAGCAGCA |
NGG |
2 |
0.0167 |
Tier II |
| 10 |
NC_000006.12 |
6 |
170370379 |
- |
FAM120B |
NNNAGGGTGATGGACCCTCA |
NGT |
2 |
0.0028 |
Tier II |
| 11 |
NC_000001.11 |
1 |
219976554 |
+ |
EPRS1 |
NNNAGGGTGATGGAGCATCA |
NGT |
2 |
0.0008 |
Tier II |
| 12 |
NC_000001.11 |
1 |
28225287 |
+ |
DNAJC8 |
NNNAGGGTGATGGACCATCA |
NAC |
2 |
0.0 |
Tier II |
| 13 |
NC_000008.11 |
8 |
24099820 |
+ |
LOC105379328 |
NNNAGGGTGTTGGACCATGA |
NGG |
2 |
0.1103 |
Tier III |
| 14 |
NC_000008.11 |
8 |
24099820 |
+ |
LOC107986931 |
NNNAGGGTGTTGGACCATGA |
NGG |
2 |
0.1103 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
43724195 |
- |
Klk13 |
NNNAGGGAGATGGACAATCA |
NGG |
2 |
0.8 |
Tier II |
| 2 |
NC_000068.7 |
2 |
27166632 |
+ |
Dbh |
NNNAGAGGGATGGACCATCA |
NGG |
2 |
0.7333 |
Tier II |
| 3 |
NC_000072.6 |
6 |
148313070 |
- |
Tmtc1 |
NNNAGAGTGATGGACCATCT |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000084.6 |
18 |
13960130 |
+ |
Zfp521 |
NNNAGGGTGATGGACAATCT |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000079.6 |
13 |
49361703 |
- |
Bicd2 |
NNNAGGTAGATGGACCATCA |
NGG |
2 |
0.4571 |
Tier II |
| 6 |
NC_000067.6 |
1 |
74989835 |
+ |
Nhej1 |
NNNAGGCTCATGGACCATCA |
NGG |
2 |
0.3702 |
Tier II |
| 7 |
NC_000075.6 |
9 |
69107109 |
+ |
Rora |
NNNAGGGAGATGGACCACCA |
NGG |
2 |
0.3429 |
Tier II |
| 8 |
NC_000071.6 |
5 |
48957854 |
+ |
Kcnip4 |
NNNAGGCTGATGGACCATCA |
NAG |
2 |
0.1782 |
Tier II |
| 9 |
NC_000068.7 |
2 |
140627223 |
+ |
Macrod2 |
NNNAGGGTGATGGTCCAGCA |
NGG |
2 |
0.1778 |
Tier II |
| 10 |
NC_000073.6 |
7 |
98900218 |
+ |
Uvrag |
NNNAGGGAGATGGCCCATCA |
NGG |
2 |
0.1714 |
Tier II |
| 11 |
NC_000075.6 |
9 |
110662907 |
+ |
Ccdc12 |
NNNAGGGTGATGGTCCATCA |
NAG |
2 |
0.1383 |
Tier II |
| 12 |
NC_000070.6 |
4 |
141074342 |
+ |
Necap2 |
NNNAGGGTGATGGGCCATCA |
NAG |
2 |
0.121 |
Tier II |
| 13 |
NC_000075.6 |
9 |
97743576 |
+ |
Clstn2 |
NNNAGGGTGATGGGACATCA |
NGG |
2 |
0.1037 |
Tier II |
| 14 |
NC_000070.6 |
4 |
129702638 |
- |
Khdrbs1 |
NNNAGGGTGATGGACTCTCA |
NGG |
2 |
0.0543 |
Tier II |
| 15 |
NC_000073.6 |
7 |
98900218 |
+ |
Gm8149 |
NNNAGGGAGATGGCCCATCA |
NGG |
2 |
0.1714 |
Tier III |
| 16 |
NC_000084.6 |
18 |
46636187 |
- |
Gm3734 |
NNNAGGCTGATGGACCATGA |
NGG |
2 |
0.0859 |
Tier III |
Other clones with same target sequence:
(none)
