Construct: sgRNA BRDN0001147883

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTGCAGGAACACTTGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA3 (2042)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76743

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 89210470 - EPHA3 NNNGCAGGAACACTTGCCAA NGG 0 1.0 Tier I
2 NC_000013.11 13 51958316 - ATP7B NNNGCTGGAACACTTGGCAA NGG 2 0.042 Tier I
3 NC_000016.10 16 75660532 - CPHXL2 NNNGCAGGAACAGCTGCCAA NGG 2 0.039 Tier I
4 NC_000005.10 5 73753499 - ARHGEF28 NNNGCAAGAAAACTTGCCAA NGG 2 0.75 Tier II
5 NC_000006.12 6 12178773 + HIVEP1 NNNAGAGGAACACTTGCCAA NGG 2 0.54 Tier II
6 NC_000001.11 1 175096491 - TNN NNNACAGGACCACTTGCCAA NGG 2 0.3 Tier II
7 NC_000002.12 2 36813609 - VIT NNNGCAGGAACCCTTGCCAA NGG 1 0.2632 Tier II
8 NC_000006.12 6 97219396 - MMS22L NNNGCAGAAAGACTTGCCAA NGG 2 0.25 Tier II
9 NC_000015.10 15 76266027 + ETFA NNNGCAGGAACAATTGCAAA NGG 2 0.2071 Tier II
10 NC_000023.11 X 8696830 - ANOS1 NNNCCAGGAACAATTGCCAA NGG 2 0.2036 Tier II
11 NC_000008.11 8 41782157 - ANK1 NNNGCAGGAACCCTTGCTAA NGG 2 0.1692 Tier II
12 NC_000010.11 10 130054038 + C10orf143 NNNGCAGGAACACTTGCCAT NAG 2 0.1556 Tier II
13 NC_000007.14 7 132505347 + PLXNA4 NNNGCACGAACACTTGCCCA NGG 2 0.1422 Tier II
14 NC_000001.11 1 14852639 + KAZN NNNGCAGGTACACTTGCCCA NGG 2 0.1241 Tier II
15 NC_000001.11 1 225118493 - DNAH14 NNNTCAGGACCACTTGCCAA NGG 2 0.1212 Tier II
16 NC_000005.10 5 35696782 + SPEF2 NNNGCAGGAACACAGGCCAA NGG 2 0.031 Tier II
17 NC_000007.14 7 55522046 + VOPP1 NNNGCAGGAACACTGGCAAA NGG 2 0.0269 Tier II
18 NC_000023.11 X 12008779 - FRMPD4 NNNGCAGGAACACTTGCCTA NTG 2 0.021 Tier II
19 NC_000008.11 8 25304866 - DOCK5 NNNGCAGGAACACTGGCCCA NGG 2 0.0103 Tier II
20 NC_000010.11 10 78690445 + LOC105378379 NNNGATGGAACACTTGCCAA NGG 2 0.4082 Tier III
21 NC_000003.12 3 118671804 - LOC105374060 NNNGGAGCAACACTTGCCAA NGG 2 0.3692 Tier III
22 NC_000004.12 4 158519920 + RXFP1-AS1 NNNTCAGGAACATTTGCCAA NGG 2 0.2545 Tier III
23 NC_000010.11 10 5038180 + LOC107984198 NNNGCAGGAACAATTGCAAA NGG 2 0.2071 Tier III
24 NC_000008.11 8 60495364 + LOC124901949 NNNGCAGGAACAATTGCGAA NGG 2 0.0513 Tier III
25 NC_000005.10 5 54740476 - LINC02998 NNNGCAGGAACACTTTCAAA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 87657342 + Hsf5 NNNGCAGGAACACTTCCCAA NGG 1 0.0 Tier I
2 NC_000074.6 8 87774227 - Zfp423 NNNGCAGGAACACATGCCAG NGG 2 0.4734 Tier II
3 NC_000074.6 8 105610813 + Ripor1 NNNCCAGGATCACTTGCCAA NGG 2 0.4671 Tier II
4 NC_000069.6 3 75251179 - Serpini2 NNNGCAGGAACACTTGACAG NGG 2 0.3569 Tier II
5 NC_000080.6 14 48462411 + Tmem260 NNNGCAGGAACATTTGTCAA NGG 2 0.3267 Tier II
6 NC_000084.6 18 71969799 - Dcc NNNGCAGGGACACTTGACAA NGG 2 0.3111 Tier II
7 NC_000072.6 6 94637166 + Lrig1 NNNACAGGAACACCTGCCAA NGG 2 0.2571 Tier II
8 NC_000074.6 8 15962991 + Csmd1 NNNGCAAGAACACTTGCCAC NGG 2 0.2273 Tier II
9 NC_000072.6 6 135221537 - Fam234b NNNGCAGGAACACTTGCAGA NGG 2 0.2019 Tier II
10 NC_000073.6 7 130952028 + Htra1 NNNGCAGGAACACTTGCAGA NGG 2 0.2019 Tier II
11 NC_000078.6 12 80667985 - Slc39a9 NNNGCAGCAACACTTGCCAA NAG 2 0.1595 Tier II
12 NC_000073.6 7 65949349 + Pcsk6 NNNGCAGGAACACTTGCAAC NGG 2 0.1224 Tier II
13 NC_000078.6 12 13340635 + Nbas NNNGAAGGAACACTTGCCCA NGG 2 0.1182 Tier II
14 NC_000072.6 6 51277833 - Gm38821 NNNCCAGGAACACTTGCCCA NGG 2 0.1095 Tier II
15 NC_000077.6 11 60840155 + Dhrs7b NNNCCAGGAACACTTGCCCA NGG 2 0.1095 Tier II
16 NC_000080.6 14 119094747 + Uggt2 NNNCCAGGAACACTTGCCCA NGG 2 0.1095 Tier II
17 NC_000072.6 6 71767119 - Reep1 NNNGCAGGACCACTTGCCAA NAG 2 0.0864 Tier II
18 NC_000069.6 3 68621707 + Schip1 NNNGCAGGACCACTTGCCCA NGG 2 0.069 Tier II
19 NC_000069.6 3 68621707 + Iqschfp NNNGCAGGACCACTTGCCCA NGG 2 0.069 Tier II
20 NC_000077.6 11 121663121 + B3gntl1 NNNGCAGGACCACTTGCCCA NGG 2 0.069 Tier II
21 NC_000071.6 5 13813781 - Gm6650 NNNGCAGGTACACTTGCCAA NGA 2 0.0417 Tier II
22 NC_000073.6 7 117442917 - Xylt1 NNNGCATGAACACTGGCCAA NGG 2 0.0286 Tier II
23 NC_000069.6 3 76635542 + Fstl5 NNNGCAGGAACAATTGGCAA NGG 2 0.0226 Tier II
24 NC_000082.6 16 4604700 - Glis2 NNNGCAGCAACACTTGCCAA NGC 2 0.0137 Tier II
25 NC_000072.6 6 90380525 + Zxdc NNNGCAGGAAAACTTGCCAA NGT 2 0.0121 Tier II
26 NC_000067.6 1 171439898 + F11r NNNGCAGGAACAGTTCCCAA NGG 2 0.0 Tier II
27 NC_000081.6 15 25469475 - Gm48957 NNNGCAGGGGCACTTGCCAA NGG 2 0.3704 Tier III
28 NC_000068.7 2 93352449 + Gm32745 NNNTCAGGAACACTTGCCAT NGG 2 0.2182 Tier III
29 NC_000075.6 9 83922695 + Gm36278 NNNGCAGGATCACTTGCCCA NGG 2 0.1826 Tier III
30 NC_000073.6 7 109444463 + 1700095J03Rik NNNCCAGGACCACTTGCCAA NGG 2 0.1765 Tier III
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Other clones with same target sequence:

(none)