Construct: sgRNA BRDN0001147901

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATAAACATTACCCATCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K2 (10746)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75511

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 127325733 + MAP3K2 NNNAAACATTACCCATCCAA NGG 0 1.0 Tier I
2 NC_000017.11 17 35548928 - SLFN14 NNNGAACATCACCCATCCAA NGG 2 0.3333 Tier I
3 NC_000010.11 10 54560887 - PCDH15 NNNAAACACAACCCATCCAA NGG 2 0.7912 Tier II
4 NC_000002.12 2 3789635 - DCDC2C NNNAAAAATTAACCATCCAA NGG 2 0.5357 Tier II
5 NC_000023.11 X 8719923 - ANOS1 NNNAAACAATACCCATCAAA NGG 2 0.5 Tier II
6 NC_000007.14 7 134084151 + EXOC4 NNNAAACATGACTCATCCAA NGG 2 0.35 Tier II
7 NC_000001.11 1 230186458 + GALNT2 NNNAAACATTACCCACACAA NGG 2 0.3111 Tier II
8 NC_000012.12 12 106802095 + RIC8B NNNAAACATCATCCATCCAA NGG 2 0.2872 Tier II
9 NC_000013.11 13 24787363 - RNF17 NNNAAACATGACCCATTCAA NGG 2 0.2333 Tier II
10 NC_000023.11 X 72663016 + PHKA1 NNNAAACATGACACATCCAA NGG 2 0.1923 Tier II
11 NC_000017.11 17 56112567 - ANKFN1 NNNAAACATTATCCAGCCAA NGG 2 0.1864 Tier II
12 NC_000016.10 16 73167111 - ZFHX3 NNNAAACATTAACCATCCAA NAG 2 0.1852 Tier II
13 NC_000012.12 12 41521950 - PDZRN4 NNNAAACACTACCCTTCCAA NGG 2 0.1846 Tier II
14 NC_000007.14 7 132296289 + PLXNA4 NNNCCACATTACCCATCCAA NGG 2 0.1765 Tier II
15 NC_000002.12 2 70552408 - TGFA NNNAAACATTACCAATACAA NGG 2 0.1633 Tier II
16 NC_000018.10 18 27976378 - CDH2 NNNAAACATTAACCATCCCA NGG 2 0.1478 Tier II
17 NC_000010.11 10 93313523 - MYOF NNNAAACATTTCACATCCAA NGG 2 0.1183 Tier II
18 NC_000001.11 1 176586056 - PAPPA2 NNNAAACATTACACCTCCAA NGG 2 0.1049 Tier II
19 NC_000014.9 14 30999803 + STRN3 NNNAAACATTACACATCCAA NAG 2 0.0997 Tier II
20 NC_000006.12 6 97256237 + MMS22L NNNAAACATTACACATCCAA NTG 2 0.015 Tier II
21 NC_000024.10 Y 12854294 - USP9Y NNNAAACATTACCCATCTAA NGC 2 0.0143 Tier II
22 NC_000005.10 5 149259710 - ABLIM3 NNNAAACATTACCCATTCAA NGT 2 0.0075 Tier II
23 NC_000007.14 7 34972821 + DPY19L1 NNNAAACATTACCCATCCCA NGT 2 0.0033 Tier II
24 NC_000003.12 3 111424208 + LOC105374039 NNNAAACAAAACCCATCCAA NGG 2 0.7959 Tier III
25 NC_000010.11 10 54560887 - LOC105378311 NNNAAACACAACCCATCCAA NGG 2 0.7912 Tier III
26 NC_000008.11 8 126622280 + LOC105375751 NNNAAACATTACCTATCCTA NGG 2 0.3949 Tier III
27 NC_000007.14 7 134084151 + LOC105375514 NNNAAACATGACTCATCCAA NGG 2 0.35 Tier III
28 NC_000017.11 17 10616762 - MYHAS NNNAAACAATACCCAGCCAA NGG 2 0.3214 Tier III
29 NC_000001.11 1 94740014 - SLC44A3-AS1 NNNAAACATCAGCCATCCAA NGG 2 0.237 Tier III
30 NC_000002.12 2 48157044 + LOC105374593 NNNAAACATGACACATCCAA NGG 2 0.1923 Tier III
31 NC_000006.12 6 109213481 - CCDC162P NNNAAACATGACCCCTCCAA NGG 2 0.1364 Tier III
32 NC_000002.12 2 52059091 - NRXN1-DT NNNAAACATTACCATTCCAA NGG 2 0.07 Tier III
33 NC_000006.12 6 37510517 - LINC02520 NNNAAACATTACCCATGCAA NAG 2 0.0153 Tier III
34 NC_000007.14 7 32638584 + DPY19L1P1 NNNAAACATTACCCATCCCA NGT 2 0.0033 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 32208717 - Map3k2 NNNCATCATTACCCATCCAA NGG 2 0.2521 Tier I
2 NC_000075.6 9 114796151 + Cmtm8 NNNAAACATTACCCAGCCAA NAG 2 0.0897 Tier I
3 NC_000082.6 16 25889869 - Trp63 NNNAAACAATAACCATCCAA NGG 2 0.6633 Tier II
4 NC_000079.6 13 81738967 - Mblac2 NNNAAATATTAACCATCCAA NGG 2 0.5804 Tier II
5 NC_000072.6 6 119594743 + Erc1 NNNAAACACTACCCATCCAT NGG 2 0.5538 Tier II
6 NC_000076.6 10 110223969 + Nav3 NNNAATCATTACTCATCCAA NGG 2 0.5 Tier II
7 NC_000076.6 10 94359441 + Tmcc3 NNNAAAGATTACCCAACCAA NGG 2 0.4278 Tier II
8 NC_000075.6 9 34649335 + Kirrel3 NNNAAACAGTACCCATCCTA NGG 2 0.3333 Tier II
9 NC_000072.6 6 62004646 - Ccser1 NNNAAAAATTACACATCCAA NGG 2 0.2885 Tier II
10 NC_000077.6 11 62626622 + Lrrc75a NNNAAACATTACCAACCCAA NGG 2 0.2333 Tier II
11 NC_000072.6 6 5803530 - Dync1i1 NNNAAACATTAACCATCCAA NAG 2 0.1852 Tier II
12 NC_000084.6 18 44012727 - Spink5 NNNAAACATTACCCATCCGA NAG 2 0.0972 Tier II
13 NC_000080.6 14 86820642 - Diaph3 NNNAAACATTACCCAGCCAA NAG 2 0.0897 Tier II
14 NC_000068.7 2 5416122 - Camk1d NNNAAACATTACCCATCCCA NAG 2 0.0536 Tier II
15 NC_000077.6 11 54518235 - Fnip1 NNNAAACATTACCCATCAAA NTG 2 0.021 Tier II
16 NC_000080.6 14 100441271 + Gm34199 NNNAAACATAACTCATCCAA NGG 2 0.6 Tier III
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Other clones with same target sequence:

(none)