Construct: sgRNA BRDN0001147914
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAACGTCTTCTTGTCACATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGGY (55277)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77110
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
59554222 |
+ |
FGGY |
NNNCGTCTTCTTGTCACATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
35842381 |
- |
NPHS1 |
NNNCGTCTTGTTGTCAGATG |
NGG |
2 |
0.0229 |
Tier I |
| 3 |
NC_000017.11 |
17 |
3884601 |
- |
CAMKK1 |
NNNCGTCTTCTCGTCACATA |
NGG |
2 |
0.8882 |
Tier II |
| 4 |
NC_000001.11 |
1 |
77009628 |
- |
ST6GALNAC5 |
NNNCAGCTTCTTGTCACATG |
NGG |
2 |
0.4952 |
Tier II |
| 5 |
NC_000006.12 |
6 |
5058255 |
+ |
LYRM4 |
NNNCTTCTTTTTGTCACATG |
NGG |
2 |
0.2824 |
Tier II |
| 6 |
NC_000003.12 |
3 |
62016507 |
+ |
PTPRG |
NNNAGTCTTCTTGTCACATG |
NAG |
2 |
0.2183 |
Tier II |
| 7 |
NC_000003.12 |
3 |
54294376 |
- |
CACNA2D3 |
NNNCGTCTTCTTGTCACCAG |
NGG |
2 |
0.0544 |
Tier II |
| 8 |
NC_000002.12 |
2 |
196412307 |
+ |
HECW2 |
NNNCGTCTTCATGTCACATG |
NTG |
2 |
0.0292 |
Tier II |
| 9 |
NC_000012.12 |
12 |
11047152 |
+ |
PRH1 |
NNNCGCCTTCTTGTCACATG |
NGC |
2 |
0.0202 |
Tier II |
| 10 |
NC_000012.12 |
12 |
11047152 |
+ |
PRH1-TAS2R14 |
NNNCGCCTTCTTGTCACATG |
NGC |
2 |
0.0202 |
Tier II |
| 11 |
NC_000023.11 |
X |
5965303 |
- |
NLGN4X |
NNNCGACTTCTTGTCTCATG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000002.12 |
2 |
233420919 |
+ |
DGKD |
NNNCGTCTTCTTGGCACATG |
NGC |
2 |
0.0 |
Tier II |
| 13 |
NC_000016.10 |
16 |
84479778 |
+ |
MEAK7 |
NNNCGTCTTCTTGGCACATG |
NGG |
1 |
0.0 |
Tier II |
| 14 |
NC_000006.12 |
6 |
5058255 |
+ |
LYRM4-AS1 |
NNNCTTCTTTTTGTCACATG |
NGG |
2 |
0.2824 |
Tier III |
| 15 |
NC_000021.9 |
21 |
10013914 |
- |
LOC105372733 |
NNNCTTCTTTTTGTCACATG |
NGG |
2 |
0.2824 |
Tier III |
| 16 |
NC_000021.9 |
21 |
18586156 |
+ |
MIR548XHG |
NNNCTTCTTCTTGTCACCTG |
NGG |
2 |
0.0571 |
Tier III |
| 17 |
NC_000012.12 |
12 |
11047152 |
+ |
PRH1-PRR4 |
NNNCGCCTTCTTGTCACATG |
NGC |
2 |
0.0202 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
95769524 |
+ |
Fggy |
NNNCATCATCTTGTCACATG |
NGG |
2 |
0.6933 |
Tier I |
| 2 |
NC_000072.6 |
6 |
127730853 |
- |
Prmt8 |
NNNTGTCTACTTGTCACATG |
NGG |
2 |
0.7429 |
Tier II |
| 3 |
NC_000080.6 |
14 |
35526011 |
+ |
Grid1 |
NNNCCTCTTCTTCTCACATG |
NGG |
2 |
0.3308 |
Tier II |
| 4 |
NC_000070.6 |
4 |
141805244 |
- |
Casp9 |
NNNGGTCTTCTTGTCAAATG |
NGG |
2 |
0.2333 |
Tier II |
| 5 |
NC_000067.6 |
1 |
32418464 |
+ |
Khdrbs2 |
NNNCGTCTTCTTGTTACATT |
NGG |
2 |
0.0467 |
Tier II |
| 6 |
NC_000084.6 |
18 |
47065436 |
- |
Commd10 |
NNNCGTCTGCTTGTGACATG |
NGG |
2 |
0.031 |
Tier II |
Other clones with same target sequence:
(none)
