Construct: sgRNA BRDN0001147942

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTACAGAACCTGTAGCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGUOK (1716)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76603

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 73938987 + DGUOK NNNCAGAACCTGTAGCAACA NGG 0 1.0 Tier I
2 NC_000001.11 1 39410989 + MACF1 NNNCAGAAGCTGTAGCAACA NGT 2 0.01 Tier I
3 NC_000003.12 3 138380744 - MRAS NNNCAGAACATGCAGCAACA NGG 2 0.6842 Tier II
4 NC_000010.11 10 25956043 - MYO3A NNNCAGAGCATGTAGCAACA NGG 2 0.6356 Tier II
5 NC_000017.11 17 10301185 + MYH13 NNNCAGCATCTGTAGCAACA NGG 2 0.3828 Tier II
6 NC_000006.12 6 70830961 - SMAP1 NNNCAGTATCTGTAGCAACA NGG 2 0.3828 Tier II
7 NC_000007.14 7 105847416 + ATXN7L1 NNNCAGGACCTCTAGCAACA NGG 2 0.3737 Tier II
8 NC_000007.14 7 29384589 + CHN2 NNNCAGAACCAGTAGCAAAA NGG 2 0.3214 Tier II
9 NC_000003.12 3 183824385 + MAP6D1 NNNCAGAACATGTAGTAACA NGG 2 0.2667 Tier II
10 NC_000002.12 2 232621344 + EFHD1 NNNCTGAGCCTGTAGCAACA NGG 2 0.2667 Tier II
11 NC_000002.12 2 85272869 + TCF7L1 NNNCAGAACCTGCAGCCACA NGG 2 0.1393 Tier II
12 NC_000007.14 7 5681547 + RNF216 NNNCAGGACCTGTAGCACCA NGG 2 0.1345 Tier II
13 NC_000015.10 15 42289167 - GANC NNNCAGAACCTGTAGCTACA NGG 1 0.1333 Tier II
14 NC_000021.9 21 43632852 - HSF2BP NNNCAGAACCTTGAGCAACA NGG 2 0.1003 Tier II
15 NC_000006.12 6 159902941 - MAS1 NNNCAGAACCTGGAGCCACA NGG 2 0.046 Tier II
16 NC_000014.9 14 89373378 - FOXN3 NNNCAGGACCTGTAGCAACA NGC 2 0.0157 Tier II
17 NC_000016.10 16 76310096 - CNTNAP4 NNNCAGAACCTGTAGCAACT NGC 2 0.0133 Tier II
18 NC_000007.14 7 70071615 - AUTS2 NNNCAGACCCTGTAGCAACA NGC 2 0.0095 Tier II
19 NC_000019.10 19 18692308 - CRTC1 NNNCAGAACCTGTAGCACCA NGT 2 0.0031 Tier II
20 NC_000017.11 17 10301185 + LOC107985004 NNNCAGCATCTGTAGCAACA NGG 2 0.3828 Tier III
21 NC_000019.10 19 27879315 + LINC02987 NNNCAGAACCTCTAGCAGCA NGG 2 0.2118 Tier III
22 NC_000024.10 Y 19050014 + TTTY14 NNNCAGAGCCTGGAGCAACA NGG 2 0.1913 Tier III
23 NC_000002.12 2 374107 - LOC105373351 NNNCAGAACCTGTAGGAATA NGG 2 0.071 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 101684756 - Traf6 NNNCAGAACCTGTGGCCACA NGG 2 0.0824 Tier I
2 NC_000074.6 8 122790582 - Acsf3 NNNCAGAACCTGTAGGAAAA NGG 2 0.0659 Tier I
3 NC_000070.6 4 44335014 + Melk NNNCAGAACATGTAGAAACA NGG 2 0.8667 Tier II
4 NC_000071.6 5 90342787 - Ankrd17 NNNTAGAACCTGTAGAAACA NGG 2 0.8 Tier II
5 NC_000085.6 19 22662266 - Trpm3 NNNCAGAACCTAAAGCAACA NGG 2 0.6462 Tier II
6 NC_000073.6 7 83616521 + Tmc3 NNNCAGAATCTGAAGCAACA NGG 2 0.6058 Tier II
7 NC_000079.6 13 81484260 - Adgrv1 NNNCAGAACCTGTGGAAACA NGG 2 0.4667 Tier II
8 NC_000074.6 8 47258943 - Stox2 NNNCAGAACCTGAAGCAACT NGG 2 0.4154 Tier II
9 NC_000073.6 7 46125299 + Abcc8 NNNCAGAACCTGTAGCATCG NGG 2 0.3824 Tier II
10 NC_000068.7 2 65525059 - Scn3a NNNCAGAACCTGCAGCAGCA NGG 2 0.3158 Tier II
11 NC_000073.6 7 143385466 - Kcnq1 NNNCAGAACCTGTAGGAACA NGG 1 0.1538 Tier II
12 NC_000082.6 16 28264039 + Fgf12 NNNCAGAACCTGTATAAACA NGG 2 0.1429 Tier II
13 NC_000068.7 2 21703838 - Gpr158 NNNGAGAACCTGTACCAACA NGG 2 0.1364 Tier II
14 NC_000074.6 8 48333815 - Tenm3 NNNCAGAACCTGTAGCAATA NAG 2 0.1197 Tier II
15 NC_000074.6 8 16995955 + Csmd1 NNNCAGAGCCTGTAGGAACA NGG 2 0.1128 Tier II
16 NC_000083.6 17 3415536 - Tiam2 NNNCAGAACCTTTAGCAACC NGG 2 0.0874 Tier II
17 NC_000068.7 2 22294378 + Myo3a NNNCAGAACCTGTATCATCA NGG 2 0.0714 Tier II
18 NC_000072.6 6 88515145 - Sec61a1 NNNCAGAACCTGTAACAACA NGA 2 0.0654 Tier II
19 NC_000081.6 15 71521908 + Fam135b NNNCAGAACCGGTAGGAACA NGG 2 0.0615 Tier II
20 NC_000083.6 17 24349896 - Abca17 NNNCAGAAACTGTAGCAACA NTG 2 0.0334 Tier II
21 NC_000075.6 9 35128620 + Dcps NNNTAGAACCTGTAGCAACA NTG 2 0.0312 Tier II
22 NC_000071.6 5 24434057 - Slc4a2 NNNCAGAACCAGTAGCAACA NTG 2 0.0292 Tier II
23 NC_000080.6 14 67772350 - Dock5 NNNCAGAACCTGTAGGCACA NGG 2 0.0271 Tier II
24 NC_000072.6 6 119269949 + Cacna2d4 NNNCAGAACCTGTACCAACA NGA 2 0.0189 Tier II
25 NC_000080.6 14 65538168 + Elp3 NNNCAGAACCTGGAGCAACA NGA 2 0.0181 Tier II
26 NC_000071.6 5 132090013 + Auts2 NNNCAGACCCTGTAGCAACA NGC 2 0.0095 Tier II
27 NC_000081.6 15 94820948 + Tmem117 NNNCAGTACCTGTAGCAACA NGT 2 0.0071 Tier II
28 NC_000070.6 4 118092438 - St3gal3 NNNCAGAACCTGTATCAACA NGC 2 0.0032 Tier II
29 NC_000073.6 7 83616521 + Gm16638 NNNCAGAATCTGAAGCAACA NGG 2 0.6058 Tier III
30 NC_000083.6 17 94772776 + 2700099C18Rik NNNCAGAACCTGAAGCAACA NGA 2 0.0481 Tier III
31 NC_000083.6 17 94772776 + Gm1976 NNNCAGAACCTGAAGCAACA NGA 2 0.0481 Tier III
32 NC_000075.6 9 35128620 + 4930581F22Rik NNNTAGAACCTGTAGCAACA NTG 2 0.0312 Tier III
33 NC_000070.6 4 48776968 - Gm52718 NNNCAGAACCTGTAGGAACA NGA 2 0.0107 Tier III
Download CSV

Other clones with same target sequence:

(none)