Construct: sgRNA BRDN0001147942
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTACAGAACCTGTAGCAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGUOK (1716)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76603
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000002.12 |
2 |
73938987 |
+ |
DGUOK |
NNNCAGAACCTGTAGCAACA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000001.11 |
1 |
39410989 |
+ |
MACF1 |
NNNCAGAAGCTGTAGCAACA |
NGT |
2 |
0.01 |
Tier I |
3 |
NC_000003.12 |
3 |
138380744 |
- |
MRAS |
NNNCAGAACATGCAGCAACA |
NGG |
2 |
0.6842 |
Tier II |
4 |
NC_000010.11 |
10 |
25956043 |
- |
MYO3A |
NNNCAGAGCATGTAGCAACA |
NGG |
2 |
0.6356 |
Tier II |
5 |
NC_000017.11 |
17 |
10301185 |
+ |
MYH13 |
NNNCAGCATCTGTAGCAACA |
NGG |
2 |
0.3828 |
Tier II |
6 |
NC_000006.12 |
6 |
70830961 |
- |
SMAP1 |
NNNCAGTATCTGTAGCAACA |
NGG |
2 |
0.3828 |
Tier II |
7 |
NC_000007.14 |
7 |
105847416 |
+ |
ATXN7L1 |
NNNCAGGACCTCTAGCAACA |
NGG |
2 |
0.3737 |
Tier II |
8 |
NC_000007.14 |
7 |
29384589 |
+ |
CHN2 |
NNNCAGAACCAGTAGCAAAA |
NGG |
2 |
0.3214 |
Tier II |
9 |
NC_000003.12 |
3 |
183824385 |
+ |
MAP6D1 |
NNNCAGAACATGTAGTAACA |
NGG |
2 |
0.2667 |
Tier II |
10 |
NC_000002.12 |
2 |
232621344 |
+ |
EFHD1 |
NNNCTGAGCCTGTAGCAACA |
NGG |
2 |
0.2667 |
Tier II |
11 |
NC_000002.12 |
2 |
85272869 |
+ |
TCF7L1 |
NNNCAGAACCTGCAGCCACA |
NGG |
2 |
0.1393 |
Tier II |
12 |
NC_000007.14 |
7 |
5681547 |
+ |
RNF216 |
NNNCAGGACCTGTAGCACCA |
NGG |
2 |
0.1345 |
Tier II |
13 |
NC_000015.10 |
15 |
42289167 |
- |
GANC |
NNNCAGAACCTGTAGCTACA |
NGG |
1 |
0.1333 |
Tier II |
14 |
NC_000021.9 |
21 |
43632852 |
- |
HSF2BP |
NNNCAGAACCTTGAGCAACA |
NGG |
2 |
0.1003 |
Tier II |
15 |
NC_000006.12 |
6 |
159902941 |
- |
MAS1 |
NNNCAGAACCTGGAGCCACA |
NGG |
2 |
0.046 |
Tier II |
16 |
NC_000014.9 |
14 |
89373378 |
- |
FOXN3 |
NNNCAGGACCTGTAGCAACA |
NGC |
2 |
0.0157 |
Tier II |
17 |
NC_000016.10 |
16 |
76310096 |
- |
CNTNAP4 |
NNNCAGAACCTGTAGCAACT |
NGC |
2 |
0.0133 |
Tier II |
18 |
NC_000007.14 |
7 |
70071615 |
- |
AUTS2 |
NNNCAGACCCTGTAGCAACA |
NGC |
2 |
0.0095 |
Tier II |
19 |
NC_000019.10 |
19 |
18692308 |
- |
CRTC1 |
NNNCAGAACCTGTAGCACCA |
NGT |
2 |
0.0031 |
Tier II |
20 |
NC_000017.11 |
17 |
10301185 |
+ |
LOC107985004 |
NNNCAGCATCTGTAGCAACA |
NGG |
2 |
0.3828 |
Tier III |
21 |
NC_000019.10 |
19 |
27879315 |
+ |
LINC02987 |
NNNCAGAACCTCTAGCAGCA |
NGG |
2 |
0.2118 |
Tier III |
22 |
NC_000024.10 |
Y |
19050014 |
+ |
TTTY14 |
NNNCAGAGCCTGGAGCAACA |
NGG |
2 |
0.1913 |
Tier III |
23 |
NC_000002.12 |
2 |
374107 |
- |
LOC105373351 |
NNNCAGAACCTGTAGGAATA |
NGG |
2 |
0.071 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000068.7 |
2 |
101684756 |
- |
Traf6 |
NNNCAGAACCTGTGGCCACA |
NGG |
2 |
0.0824 |
Tier I |
2 |
NC_000074.6 |
8 |
122790582 |
- |
Acsf3 |
NNNCAGAACCTGTAGGAAAA |
NGG |
2 |
0.0659 |
Tier I |
3 |
NC_000070.6 |
4 |
44335014 |
+ |
Melk |
NNNCAGAACATGTAGAAACA |
NGG |
2 |
0.8667 |
Tier II |
4 |
NC_000071.6 |
5 |
90342787 |
- |
Ankrd17 |
NNNTAGAACCTGTAGAAACA |
NGG |
2 |
0.8 |
Tier II |
5 |
NC_000085.6 |
19 |
22662266 |
- |
Trpm3 |
NNNCAGAACCTAAAGCAACA |
NGG |
2 |
0.6462 |
Tier II |
6 |
NC_000073.6 |
7 |
83616521 |
+ |
Tmc3 |
NNNCAGAATCTGAAGCAACA |
NGG |
2 |
0.6058 |
Tier II |
7 |
NC_000079.6 |
13 |
81484260 |
- |
Adgrv1 |
NNNCAGAACCTGTGGAAACA |
NGG |
2 |
0.4667 |
Tier II |
8 |
NC_000074.6 |
8 |
47258943 |
- |
Stox2 |
NNNCAGAACCTGAAGCAACT |
NGG |
2 |
0.4154 |
Tier II |
9 |
NC_000073.6 |
7 |
46125299 |
+ |
Abcc8 |
NNNCAGAACCTGTAGCATCG |
NGG |
2 |
0.3824 |
Tier II |
10 |
NC_000068.7 |
2 |
65525059 |
- |
Scn3a |
NNNCAGAACCTGCAGCAGCA |
NGG |
2 |
0.3158 |
Tier II |
11 |
NC_000073.6 |
7 |
143385466 |
- |
Kcnq1 |
NNNCAGAACCTGTAGGAACA |
NGG |
1 |
0.1538 |
Tier II |
12 |
NC_000082.6 |
16 |
28264039 |
+ |
Fgf12 |
NNNCAGAACCTGTATAAACA |
NGG |
2 |
0.1429 |
Tier II |
13 |
NC_000068.7 |
2 |
21703838 |
- |
Gpr158 |
NNNGAGAACCTGTACCAACA |
NGG |
2 |
0.1364 |
Tier II |
14 |
NC_000074.6 |
8 |
48333815 |
- |
Tenm3 |
NNNCAGAACCTGTAGCAATA |
NAG |
2 |
0.1197 |
Tier II |
15 |
NC_000074.6 |
8 |
16995955 |
+ |
Csmd1 |
NNNCAGAGCCTGTAGGAACA |
NGG |
2 |
0.1128 |
Tier II |
16 |
NC_000083.6 |
17 |
3415536 |
- |
Tiam2 |
NNNCAGAACCTTTAGCAACC |
NGG |
2 |
0.0874 |
Tier II |
17 |
NC_000068.7 |
2 |
22294378 |
+ |
Myo3a |
NNNCAGAACCTGTATCATCA |
NGG |
2 |
0.0714 |
Tier II |
18 |
NC_000072.6 |
6 |
88515145 |
- |
Sec61a1 |
NNNCAGAACCTGTAACAACA |
NGA |
2 |
0.0654 |
Tier II |
19 |
NC_000081.6 |
15 |
71521908 |
+ |
Fam135b |
NNNCAGAACCGGTAGGAACA |
NGG |
2 |
0.0615 |
Tier II |
20 |
NC_000083.6 |
17 |
24349896 |
- |
Abca17 |
NNNCAGAAACTGTAGCAACA |
NTG |
2 |
0.0334 |
Tier II |
21 |
NC_000075.6 |
9 |
35128620 |
+ |
Dcps |
NNNTAGAACCTGTAGCAACA |
NTG |
2 |
0.0312 |
Tier II |
22 |
NC_000071.6 |
5 |
24434057 |
- |
Slc4a2 |
NNNCAGAACCAGTAGCAACA |
NTG |
2 |
0.0292 |
Tier II |
23 |
NC_000080.6 |
14 |
67772350 |
- |
Dock5 |
NNNCAGAACCTGTAGGCACA |
NGG |
2 |
0.0271 |
Tier II |
24 |
NC_000072.6 |
6 |
119269949 |
+ |
Cacna2d4 |
NNNCAGAACCTGTACCAACA |
NGA |
2 |
0.0189 |
Tier II |
25 |
NC_000080.6 |
14 |
65538168 |
+ |
Elp3 |
NNNCAGAACCTGGAGCAACA |
NGA |
2 |
0.0181 |
Tier II |
26 |
NC_000071.6 |
5 |
132090013 |
+ |
Auts2 |
NNNCAGACCCTGTAGCAACA |
NGC |
2 |
0.0095 |
Tier II |
27 |
NC_000081.6 |
15 |
94820948 |
+ |
Tmem117 |
NNNCAGTACCTGTAGCAACA |
NGT |
2 |
0.0071 |
Tier II |
28 |
NC_000070.6 |
4 |
118092438 |
- |
St3gal3 |
NNNCAGAACCTGTATCAACA |
NGC |
2 |
0.0032 |
Tier II |
29 |
NC_000073.6 |
7 |
83616521 |
+ |
Gm16638 |
NNNCAGAATCTGAAGCAACA |
NGG |
2 |
0.6058 |
Tier III |
30 |
NC_000083.6 |
17 |
94772776 |
+ |
2700099C18Rik |
NNNCAGAACCTGAAGCAACA |
NGA |
2 |
0.0481 |
Tier III |
31 |
NC_000083.6 |
17 |
94772776 |
+ |
Gm1976 |
NNNCAGAACCTGAAGCAACA |
NGA |
2 |
0.0481 |
Tier III |
32 |
NC_000075.6 |
9 |
35128620 |
+ |
4930581F22Rik |
NNNTAGAACCTGTAGCAACA |
NTG |
2 |
0.0312 |
Tier III |
33 |
NC_000070.6 |
4 |
48776968 |
- |
Gm52718 |
NNNCAGAACCTGTAGGAACA |
NGA |
2 |
0.0107 |
Tier III |
Other clones with same target sequence:
(none)