Construct: sgRNA BRDN0001147958
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTGCACAAGCAGACCAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IKBKE (9641)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77770
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
206478981 |
+ |
IKBKE |
NNNGCACAAGCAGACCAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
7229112 |
+ |
RREB1 |
NNNGCACAAGCAGACCCATG |
NGG |
2 |
0.1222 |
Tier I |
| 3 |
NC_000002.12 |
2 |
240621134 |
- |
GPR35 |
NNNGCTCAGGCAGACCAGTG |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000007.14 |
7 |
50755591 |
+ |
GRB10 |
NNNGCACAAGGAGACCAGTG |
NGG |
1 |
0.25 |
Tier II |
| 5 |
NC_000017.11 |
17 |
34613993 |
+ |
TMEM132E |
NNNCCACAAGCACACCAGTG |
NGG |
2 |
0.2229 |
Tier II |
| 6 |
NC_000006.12 |
6 |
118246920 |
- |
SLC35F1 |
NNNGCATAAGGAGACCAGTG |
NGG |
2 |
0.2031 |
Tier II |
| 7 |
NC_000022.11 |
22 |
28620677 |
+ |
TTC28 |
NNNGCAAAAGCAGACCAGTG |
NAG |
2 |
0.1944 |
Tier II |
| 8 |
NC_000001.11 |
1 |
222581824 |
+ |
TAF1A |
NNNGAACAAGGAGACCAGTG |
NGG |
2 |
0.1429 |
Tier II |
| 9 |
NC_000020.11 |
20 |
59626578 |
+ |
PHACTR3 |
NNNGAACAAGGAGACCAGTG |
NGG |
2 |
0.1429 |
Tier II |
| 10 |
NC_000001.11 |
1 |
152758578 |
+ |
KPRP |
NNNACACAAGCAGACGAGTG |
NGG |
2 |
0.1385 |
Tier II |
| 11 |
NC_000008.11 |
8 |
84350497 |
- |
RALYL |
NNNCCACAAGCAGACCAGTG |
NAG |
2 |
0.1373 |
Tier II |
| 12 |
NC_000002.12 |
2 |
114901924 |
+ |
DPP10 |
NNNGCACAAGCAGACCAGTC |
NAG |
2 |
0.1111 |
Tier II |
| 13 |
NC_000015.10 |
15 |
57290639 |
+ |
TCF12 |
NNNGCCCAAGCAGCCCAGTG |
NGG |
2 |
0.0974 |
Tier II |
| 14 |
NC_000003.12 |
3 |
46985145 |
+ |
NBEAL2 |
NNNGCACAGGCAGACCTGTG |
NGG |
2 |
0.0889 |
Tier II |
| 15 |
NC_000001.11 |
1 |
165859966 |
- |
UCK2 |
NNNGCACAAGGAGACTAGTG |
NGG |
2 |
0.0769 |
Tier II |
| 16 |
NC_000010.11 |
10 |
88669189 |
- |
LIPF |
NNNGCACAAGAAGACCAGTG |
NGA |
2 |
0.0521 |
Tier II |
| 17 |
NC_000009.12 |
9 |
79640987 |
+ |
TLE4 |
NNNGGACAAGCAGATCAGTG |
NGG |
2 |
0.04 |
Tier II |
| 18 |
NC_000001.11 |
1 |
203344170 |
+ |
FMOD |
NNNGTACAAGCAGAGCAGTG |
NGG |
2 |
0.0318 |
Tier II |
| 19 |
NC_000011.10 |
11 |
68745727 |
- |
TESMIN |
NNNGCCCAAGCAGATCAGTG |
NGG |
2 |
0.0303 |
Tier II |
| 20 |
NC_000007.14 |
7 |
35005404 |
+ |
DPY19L1 |
NNNGCACAAGCAGAGCAGTG |
NGA |
2 |
0.0035 |
Tier II |
| 21 |
NC_000018.10 |
18 |
73759463 |
+ |
LOC105372191 |
NNNGCACAAGCAGACCAGTG |
NAG |
1 |
0.2593 |
Tier III |
| 22 |
NC_000002.12 |
2 |
87257658 |
+ |
LOC107985908 |
NNNGCAGAAGCAGACCAGTC |
NGG |
2 |
0.2017 |
Tier III |
| 23 |
NC_000006.12 |
6 |
39963025 |
+ |
LOC102723789 |
NNNGCAAAAGCAGACCAGTG |
NAG |
2 |
0.1944 |
Tier III |
| 24 |
NC_000020.11 |
20 |
59626578 |
+ |
PHACTR3-AS1 |
NNNGAACAAGGAGACCAGTG |
NGG |
2 |
0.1429 |
Tier III |
| 25 |
NC_000008.11 |
8 |
93478210 |
+ |
CIBAR1-DT |
NNNGCACAAGGACACCAGTG |
NGG |
2 |
0.1053 |
Tier III |
| 26 |
NC_000012.12 |
12 |
42623957 |
+ |
LINC02402 |
NNNGCACAAGCAGACCTGTT |
NGG |
2 |
0.0933 |
Tier III |
| 27 |
NC_000007.14 |
7 |
32670859 |
+ |
DPY19L1P1 |
NNNGCACAAGCAGAGCAGTG |
NGA |
2 |
0.0035 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
118158544 |
+ |
Mdm1 |
NNNGGACAAGCAGACCTGTG |
NGG |
2 |
0.08 |
Tier I |
| 2 |
NC_000083.6 |
17 |
24207333 |
- |
Ntn3 |
NNNGCTAAAGCAGACCAGTG |
NGG |
2 |
0.5357 |
Tier II |
| 3 |
NC_000078.6 |
12 |
85290947 |
- |
Zc2hc1c |
NNNCCACAAGCAAACCAGTG |
NGG |
2 |
0.4887 |
Tier II |
| 4 |
NC_000083.6 |
17 |
31469977 |
+ |
Pde9a |
NNNGAAAAAGCAGACCAGTG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000083.6 |
17 |
6558698 |
- |
Gm29721 |
NNNACAGAAGCAGACCAGTG |
NGG |
2 |
0.4235 |
Tier II |
| 6 |
NC_000083.6 |
17 |
6692904 |
+ |
Sytl3 |
NNNACAGAAGCAGACCAGTG |
NGG |
2 |
0.4235 |
Tier II |
| 7 |
NC_000070.6 |
4 |
46983512 |
- |
Gabbr2 |
NNNCCTCAAGCAGACCAGTG |
NGG |
2 |
0.3782 |
Tier II |
| 8 |
NC_000078.6 |
12 |
13316320 |
- |
Nbas |
NNNGCATAAGCACACCAGTG |
NGG |
2 |
0.3421 |
Tier II |
| 9 |
NC_000068.7 |
2 |
91700468 |
+ |
Atg13 |
NNNGCTCAAGCAGACCACTG |
NGG |
2 |
0.3401 |
Tier II |
| 10 |
NC_000069.6 |
3 |
121784228 |
+ |
Abcd3 |
NNNGCACAAGCAGACCAGTG |
NAG |
1 |
0.2593 |
Tier II |
| 11 |
NC_000071.6 |
5 |
108644505 |
- |
Tmem175 |
NNNGGACTAGCAGACCAGTG |
NGG |
2 |
0.2571 |
Tier II |
| 12 |
NC_000074.6 |
8 |
39058583 |
+ |
Tusc3 |
NNNGCACAAGCAGAAAAGTG |
NGG |
2 |
0.2222 |
Tier II |
| 13 |
NC_000075.6 |
9 |
123421553 |
+ |
Lars2 |
NNNTCACATGCAGACCAGTG |
NGG |
2 |
0.2182 |
Tier II |
| 14 |
NC_000071.6 |
5 |
38414262 |
- |
Slc2a9 |
NNNGCACACGCAGACCAGGG |
NGG |
2 |
0.1429 |
Tier II |
| 15 |
NC_000068.7 |
2 |
29362693 |
+ |
Med27 |
NNNGCACAACCAGACTAGTG |
NGG |
2 |
0.1231 |
Tier II |
| 16 |
NC_000079.6 |
13 |
49272010 |
+ |
Fgd3 |
NNNGCACAAGCAGACTAGTG |
NAG |
2 |
0.0798 |
Tier II |
| 17 |
NC_000083.6 |
17 |
56473930 |
+ |
Ptprs |
NNNTCACAAGCAGACGAGTG |
NGG |
2 |
0.0559 |
Tier II |
| 18 |
NC_000077.6 |
11 |
33429212 |
+ |
Ranbp17 |
NNNGCACAAGCAGCACAGTG |
NGG |
2 |
0.0476 |
Tier II |
| 19 |
NC_000083.6 |
17 |
31327489 |
+ |
Slc37a1 |
NNNGAACAAGCAGATCAGTG |
NGG |
2 |
0.0381 |
Tier II |
| 20 |
NC_000077.6 |
11 |
117280087 |
+ |
Septin9 |
NNNGCACAGGCAGAGCAGTG |
NGG |
2 |
0.0333 |
Tier II |
| 21 |
NC_000068.7 |
2 |
11773098 |
+ |
Fbh1 |
NNNGCACAAGCAGACGGGTG |
NGG |
2 |
0.0271 |
Tier II |
| 22 |
NC_000083.6 |
17 |
6391906 |
- |
Gm29719 |
NNNACAGAAGCAGACCAGTG |
NGG |
2 |
0.4235 |
Tier III |
| 23 |
NC_000078.6 |
12 |
112227974 |
- |
Gm36894 |
NNNGCACAACAAGACCAGTG |
NGG |
2 |
0.3 |
Tier III |
| 24 |
NC_000079.6 |
13 |
3361303 |
+ |
Gm16505 |
NNNGCACTCGCAGACCAGTG |
NGG |
2 |
0.2449 |
Tier III |
| 25 |
NC_000083.6 |
17 |
56473930 |
+ |
Gm32822 |
NNNTCACAAGCAGACGAGTG |
NGG |
2 |
0.0559 |
Tier III |
Other clones with same target sequence:
(none)
