Construct: sgRNA BRDN0001147987
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGATCGTTATGGAGTACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK4 (6789)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76075
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
44981895 |
+ |
STK4 |
NNNATCGTTATGGAGTACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
98749325 |
- |
STK3 |
NNNATTGTTATGGAGTACTG |
NGG |
1 |
0.9286 |
Tier I |
| 3 |
NC_000002.12 |
2 |
190916352 |
- |
GLS |
NNNATCATTATGGAGTAGTG |
NGG |
2 |
0.1333 |
Tier II |
| 4 |
NC_000006.12 |
6 |
148369477 |
+ |
SASH1 |
NNNATCGTTATGGAGTTCTG |
NGT |
2 |
0.0022 |
Tier II |
| 5 |
NC_000008.11 |
8 |
90594501 |
+ |
LINC01030 |
NNNATCATTATGGAGAACTG |
NGG |
2 |
0.9091 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
164086561 |
+ |
Stk4 |
NNNATTGTTATGGAGTACTG |
NGG |
1 |
0.9286 |
Tier I |
| 2 |
NC_000081.6 |
15 |
35099483 |
- |
Stk3 |
NNNATTGTTATGGAGTACTG |
NGG |
1 |
0.9286 |
Tier I |
| 3 |
NC_000067.6 |
1 |
86925628 |
+ |
Dis3l2 |
NNNATAGTAATGGAGTACTG |
NGG |
2 |
0.8622 |
Tier II |
| 4 |
NC_000074.6 |
8 |
28812644 |
+ |
Unc5d |
NNNATCTTTATGGAGAACTG |
NGG |
2 |
0.5195 |
Tier II |
| 5 |
NC_000076.6 |
10 |
123733943 |
- |
Tafa2 |
NNNCTTGTTATGGAGTACTG |
NGG |
2 |
0.3277 |
Tier II |
| 6 |
NC_000079.6 |
13 |
81212512 |
- |
Adgrv1 |
NNNTTGGTTATGGAGTACTG |
NGG |
2 |
0.3182 |
Tier II |
| 7 |
NC_000083.6 |
17 |
49843206 |
+ |
Kif6 |
NNNATGGTTATGGAGGACTG |
NGG |
2 |
0.1731 |
Tier II |
| 8 |
NC_000067.6 |
1 |
4824942 |
- |
Lypla1 |
NNNATCTTTATGGAGTACTG |
NGT |
2 |
0.0092 |
Tier II |
| 9 |
NC_000080.6 |
14 |
115936372 |
- |
Gpc5 |
NNNATCTTTATGGAGTACTG |
NGT |
2 |
0.0092 |
Tier II |
| 10 |
NC_000067.6 |
1 |
86925628 |
+ |
Gm41909 |
NNNATAGTAATGGAGTACTG |
NGG |
2 |
0.8622 |
Tier III |
Other clones with same target sequence:
(none)
