Construct: sgRNA BRDN0001148029
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTGTGGCCGGCAGACTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LIMK1 (3984)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76048
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
74107106 |
- |
LIMK1 |
NNNGTGGCCGGCAGACTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
50028356 |
+ |
TTLL8 |
NNNGTGGCAGGCAGACCACG |
NGG |
2 |
0.605 |
Tier II |
| 3 |
NC_000005.10 |
5 |
123000098 |
- |
SNX24 |
NNNGTGGCCGGCAGGCTACT |
NGG |
2 |
0.455 |
Tier II |
| 4 |
NC_000002.12 |
2 |
33254120 |
- |
LTBP1 |
NNNGAGGCCGGCAGACCACG |
NGG |
2 |
0.3529 |
Tier II |
| 5 |
NC_000006.12 |
6 |
82986253 |
+ |
UBE3D |
NNNGTGGCGGGCAGATTACG |
NGG |
2 |
0.1905 |
Tier II |
| 6 |
NC_000009.12 |
9 |
108128189 |
+ |
LOC105376214 |
NNNGTGGCGGGCAGACCACG |
NGG |
2 |
0.437 |
Tier III |
| 7 |
NC_000013.11 |
13 |
87379943 |
- |
LIN28AP2 |
NNNGAGGCAGGCAGACTACG |
NGG |
2 |
0.4286 |
Tier III |
| 8 |
NC_000013.11 |
13 |
87379943 |
- |
LOC105370302 |
NNNGAGGCAGGCAGACTACG |
NGG |
2 |
0.4286 |
Tier III |
| 9 |
NC_000001.11 |
1 |
143911577 |
- |
LOC105371209 |
NNNGAGGCCGGCAGATTACG |
NGG |
2 |
0.1538 |
Tier III |
| 10 |
NC_000017.11 |
17 |
30780029 |
- |
SUZ12P1 |
NNNGAGGCCGGCAGATTACG |
NGG |
2 |
0.1538 |
Tier III |
| 11 |
NC_000010.11 |
10 |
128797678 |
+ |
LOC105378555 |
NNNGTGGCCGGCAGAATACG |
NGC |
2 |
0.0222 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
77979546 |
- |
Elovl5 |
NNNGTGGCCGGCAGACTGGG |
NGG |
2 |
0.05 |
Tier II |
| 2 |
NC_000072.6 |
6 |
118080307 |
- |
Rasgef1a |
NNNGTGGCCGGCAGTCTACG |
NGC |
2 |
0.0044 |
Tier II |
Other clones with same target sequence:
(none)
