Construct: sgRNA BRDN0001148030
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTTCGGTAGCATCTTGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K10 (4294)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76005
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
40205171 |
+ |
MAP3K10 |
NNNTCGGTAGCATCTTGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
7642214 |
- |
CAMTA1 |
NNNTCGGCAGCATTTTGAAG |
NGG |
2 |
0.7333 |
Tier II |
| 3 |
NC_000001.11 |
1 |
233183185 |
- |
PCNX2 |
NNNCCGGTAGCATCTTGAAG |
NGG |
1 |
0.6471 |
Tier II |
| 4 |
NC_000019.10 |
19 |
44065191 |
- |
ZNF223 |
NNNTCTGTAGAATCTTGAAG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000003.12 |
3 |
107652893 |
- |
BBX |
NNNCCTGTAGCATCTTGAAG |
NGG |
2 |
0.4314 |
Tier II |
| 6 |
NC_000002.12 |
2 |
167464267 |
+ |
B3GALT1 |
NNNTCAGTAGTATCTTGAAG |
NGG |
2 |
0.3077 |
Tier II |
| 7 |
NC_000012.12 |
12 |
8052995 |
- |
FOXJ2 |
NNNTCGGCAGCATCTTGCAG |
NGG |
2 |
0.1905 |
Tier II |
| 8 |
NC_000019.10 |
19 |
56696357 |
- |
LOC105372472 |
NNNACGGTAGCATCTTGAAC |
NGG |
2 |
0.2041 |
Tier III |
| 9 |
NC_000019.10 |
19 |
56696357 |
- |
LOC105372473 |
NNNACGGTAGCATCTTGAAC |
NGG |
2 |
0.2041 |
Tier III |
| 10 |
NC_000013.11 |
13 |
63729416 |
+ |
LINC00395 |
NNNTCGGTAGCATCTGGAAG |
NGA |
2 |
0.024 |
Tier III |
| 11 |
NC_000013.11 |
13 |
63729416 |
+ |
LOC105370235 |
NNNTCGGTAGCATCTGGAAG |
NGA |
2 |
0.024 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
25227972 |
- |
Fhl5 |
NNNTCGGTAGCATTTTGAAG |
NGG |
1 |
0.7333 |
Tier II |
| 2 |
NC_000075.6 |
9 |
97760425 |
+ |
Clstn2 |
NNNTCTGTAACATCTTGAAG |
NGG |
2 |
0.6222 |
Tier II |
| 3 |
NC_000076.6 |
10 |
54052189 |
+ |
Man1a |
NNNTCGGTAGCATCATGAAG |
NGC |
2 |
0.0129 |
Tier II |
| 4 |
NC_000078.6 |
12 |
73503462 |
- |
Gm34016 |
NNNTTGATAGCATCTTGAAG |
NGG |
2 |
0.6364 |
Tier III |
Other clones with same target sequence:
(none)
