Construct: sgRNA BRDN0001148035
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGGATTGTCAAAAATGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK1D (57118)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77442
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000010.11 |
10 |
12769747 |
+ |
CAMK1D |
NNNGGATTGTCAAAAATGGA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000011.10 |
11 |
31266554 |
- |
DCDC1 |
NNNGAATAGTCAAAAATGGA |
NGG |
2 |
0.6933 |
Tier II |
3 |
NC_000016.10 |
16 |
29982319 |
+ |
TAOK2 |
NNNGAATTATCAAAAATGGA |
NGG |
2 |
0.5571 |
Tier II |
4 |
NC_000005.10 |
5 |
21789615 |
- |
CDH12 |
NNNGGATTTTCAAAAATGAA |
NGG |
2 |
0.381 |
Tier II |
5 |
NC_000001.11 |
1 |
50488885 |
- |
FAF1 |
NNNGGACTGTCAATAATGGA |
NGG |
2 |
0.3667 |
Tier II |
6 |
NC_000001.11 |
1 |
180244306 |
- |
LHX4 |
NNNGGCTTGGCAAAAATGGA |
NGG |
2 |
0.2273 |
Tier II |
7 |
NC_000006.12 |
6 |
7812163 |
+ |
BMP6 |
NNNGGATTGAGAAAAATGGA |
NGG |
2 |
0.2143 |
Tier II |
8 |
NC_000002.12 |
2 |
11167163 |
+ |
SLC66A3 |
NNNGGCTTGTCAAGAATGGA |
NGG |
2 |
0.2121 |
Tier II |
9 |
NC_000001.11 |
1 |
53944763 |
- |
IFT25 |
NNNGGATTGTAAAAAATGGA |
NAG |
2 |
0.1944 |
Tier II |
10 |
NC_000003.12 |
3 |
85074886 |
- |
CADM2 |
NNNGGATTTTTAAAAATGGA |
NGG |
2 |
0.1641 |
Tier II |
11 |
NC_000022.11 |
22 |
29672266 |
- |
NF2 |
NNNGGATTGTTACAAATGGA |
NGG |
2 |
0.0648 |
Tier II |
12 |
NC_000001.11 |
1 |
77188654 |
- |
PIGK |
NNNGGATTTTCAAAAAGGGA |
NGG |
2 |
0.0627 |
Tier II |
13 |
NC_000001.11 |
1 |
232573502 |
- |
SIPA1L2 |
NNNGGATTGTCAAAAATGGA |
NTC |
2 |
0.0 |
Tier II |
14 |
NC_000011.10 |
11 |
115676913 |
- |
LINC02698 |
NNNGAATTGTCAAAAATGGG |
NGG |
2 |
0.6627 |
Tier III |
15 |
NC_000021.9 |
21 |
13550499 |
+ |
LINC01674 |
NNNGGAGTGTCAAAAATTGA |
NGG |
2 |
0.3922 |
Tier III |
16 |
NC_000005.10 |
5 |
128688588 |
+ |
LOC105379168 |
NNNGGATTGTCAAAAAAGAA |
NGG |
2 |
0.381 |
Tier III |
17 |
NC_000004.12 |
4 |
148714248 |
- |
LOC107986195 |
NNNAGATTGTCAAAAAGGGA |
NGG |
2 |
0.1059 |
Tier III |
18 |
NC_000012.12 |
12 |
84101965 |
- |
LOC107984536 |
NNNGGATTTTCAAAAGTGGA |
NGG |
2 |
0.1026 |
Tier III |
19 |
NC_000012.12 |
12 |
94518670 |
- |
LOC102724960 |
NNNGGATTGTGAACAATGGA |
NGG |
2 |
0.0536 |
Tier III |
20 |
NC_000015.10 |
15 |
90667549 |
+ |
CRTC3-AS1 |
NNNGGATTGTAAAAAATGGA |
NTG |
2 |
0.0292 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000068.7 |
2 |
5354722 |
- |
Camk1d |
NNNGGCTTGTCGAAAATGGA |
NGG |
2 |
0.3283 |
Tier I |
2 |
NC_000073.6 |
7 |
124099443 |
- |
Hs3st4 |
NNNGGATTGTCAAAAATGAA |
NGG |
1 |
0.7143 |
Tier II |
3 |
NC_000070.6 |
4 |
104394543 |
- |
Dab1 |
NNNGGATGGTCAAAAATGAA |
NGG |
2 |
0.5238 |
Tier II |
4 |
NC_000083.6 |
17 |
14382478 |
+ |
Smoc2 |
NNNGGATAGCCAAAAATGGA |
NGG |
2 |
0.4267 |
Tier II |
5 |
NC_000067.6 |
1 |
159909542 |
- |
Tnr |
NNNGGAGTGTCAAAGATGGA |
NGG |
2 |
0.3824 |
Tier II |
6 |
NC_000080.6 |
14 |
57580594 |
+ |
Xpo4 |
NNNGGATTCTCAAAAATGTA |
NGG |
2 |
0.359 |
Tier II |
7 |
NC_000078.6 |
12 |
71924380 |
+ |
Daam1 |
NNNGGATTTTCAAGAATGGA |
NGG |
2 |
0.2489 |
Tier II |
8 |
NC_000086.7 |
X |
163925943 |
+ |
Ap1s2 |
NNNGGATTGTCCAAAAAGGA |
NGG |
2 |
0.1404 |
Tier II |
9 |
NC_000069.6 |
3 |
9253783 |
+ |
Zbtb10 |
NNNGGCTTGTGAAAAATGGA |
NGG |
2 |
0.1136 |
Tier II |
10 |
NC_000067.6 |
1 |
74414107 |
- |
Vil1 |
NNNGGAGTGTCAAAAGTGGA |
NGG |
2 |
0.1131 |
Tier II |
11 |
NC_000080.6 |
14 |
35582295 |
- |
Grid1 |
NNNGGATTTTCAAAAGTGGA |
NGG |
2 |
0.1026 |
Tier II |
12 |
NC_000073.6 |
7 |
73990034 |
- |
St8sia2 |
NNNGGATTGTCAATAATGGA |
NGC |
2 |
0.0119 |
Tier II |
13 |
NC_000077.6 |
11 |
89382984 |
+ |
Gm42088 |
NNNGGATGGTAAAAAATGGA |
NGG |
2 |
0.55 |
Tier III |
14 |
NC_000072.6 |
6 |
69494581 |
+ |
Igk |
NNNGGATTATCAAGAATGGA |
NGG |
2 |
0.3 |
Tier III |
15 |
NC_000072.6 |
6 |
69675837 |
+ |
Igk |
NNNGGATTATCAAGAATGGA |
NGG |
2 |
0.3 |
Tier III |
16 |
NC_000077.6 |
11 |
41897616 |
- |
A230004M16Rik |
NNNGGATTGTGAAAAATGGA |
NTG |
2 |
0.0097 |
Tier III |
Other clones with same target sequence:
(none)