Construct: sgRNA BRDN0001148035

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTGGATTGTCAAAAATGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK1D (57118)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77442

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 12769747 + CAMK1D NNNGGATTGTCAAAAATGGA NGG 0 1.0 Tier I
2 NC_000011.10 11 31266554 - DCDC1 NNNGAATAGTCAAAAATGGA NGG 2 0.6933 Tier II
3 NC_000016.10 16 29982319 + TAOK2 NNNGAATTATCAAAAATGGA NGG 2 0.5571 Tier II
4 NC_000005.10 5 21789615 - CDH12 NNNGGATTTTCAAAAATGAA NGG 2 0.381 Tier II
5 NC_000001.11 1 50488885 - FAF1 NNNGGACTGTCAATAATGGA NGG 2 0.3667 Tier II
6 NC_000001.11 1 180244306 - LHX4 NNNGGCTTGGCAAAAATGGA NGG 2 0.2273 Tier II
7 NC_000006.12 6 7812163 + BMP6 NNNGGATTGAGAAAAATGGA NGG 2 0.2143 Tier II
8 NC_000002.12 2 11167163 + SLC66A3 NNNGGCTTGTCAAGAATGGA NGG 2 0.2121 Tier II
9 NC_000001.11 1 53944763 - IFT25 NNNGGATTGTAAAAAATGGA NAG 2 0.1944 Tier II
10 NC_000003.12 3 85074886 - CADM2 NNNGGATTTTTAAAAATGGA NGG 2 0.1641 Tier II
11 NC_000022.11 22 29672266 - NF2 NNNGGATTGTTACAAATGGA NGG 2 0.0648 Tier II
12 NC_000001.11 1 77188654 - PIGK NNNGGATTTTCAAAAAGGGA NGG 2 0.0627 Tier II
13 NC_000001.11 1 232573502 - SIPA1L2 NNNGGATTGTCAAAAATGGA NTC 2 0.0 Tier II
14 NC_000011.10 11 115676913 - LINC02698 NNNGAATTGTCAAAAATGGG NGG 2 0.6627 Tier III
15 NC_000021.9 21 13550499 + LINC01674 NNNGGAGTGTCAAAAATTGA NGG 2 0.3922 Tier III
16 NC_000005.10 5 128688588 + LOC105379168 NNNGGATTGTCAAAAAAGAA NGG 2 0.381 Tier III
17 NC_000004.12 4 148714248 - LOC107986195 NNNAGATTGTCAAAAAGGGA NGG 2 0.1059 Tier III
18 NC_000012.12 12 84101965 - LOC107984536 NNNGGATTTTCAAAAGTGGA NGG 2 0.1026 Tier III
19 NC_000012.12 12 94518670 - LOC102724960 NNNGGATTGTGAACAATGGA NGG 2 0.0536 Tier III
20 NC_000015.10 15 90667549 + CRTC3-AS1 NNNGGATTGTAAAAAATGGA NTG 2 0.0292 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 5354722 - Camk1d NNNGGCTTGTCGAAAATGGA NGG 2 0.3283 Tier I
2 NC_000073.6 7 124099443 - Hs3st4 NNNGGATTGTCAAAAATGAA NGG 1 0.7143 Tier II
3 NC_000070.6 4 104394543 - Dab1 NNNGGATGGTCAAAAATGAA NGG 2 0.5238 Tier II
4 NC_000083.6 17 14382478 + Smoc2 NNNGGATAGCCAAAAATGGA NGG 2 0.4267 Tier II
5 NC_000067.6 1 159909542 - Tnr NNNGGAGTGTCAAAGATGGA NGG 2 0.3824 Tier II
6 NC_000080.6 14 57580594 + Xpo4 NNNGGATTCTCAAAAATGTA NGG 2 0.359 Tier II
7 NC_000078.6 12 71924380 + Daam1 NNNGGATTTTCAAGAATGGA NGG 2 0.2489 Tier II
8 NC_000086.7 X 163925943 + Ap1s2 NNNGGATTGTCCAAAAAGGA NGG 2 0.1404 Tier II
9 NC_000069.6 3 9253783 + Zbtb10 NNNGGCTTGTGAAAAATGGA NGG 2 0.1136 Tier II
10 NC_000067.6 1 74414107 - Vil1 NNNGGAGTGTCAAAAGTGGA NGG 2 0.1131 Tier II
11 NC_000080.6 14 35582295 - Grid1 NNNGGATTTTCAAAAGTGGA NGG 2 0.1026 Tier II
12 NC_000073.6 7 73990034 - St8sia2 NNNGGATTGTCAATAATGGA NGC 2 0.0119 Tier II
13 NC_000077.6 11 89382984 + Gm42088 NNNGGATGGTAAAAAATGGA NGG 2 0.55 Tier III
14 NC_000072.6 6 69494581 + Igk NNNGGATTATCAAGAATGGA NGG 2 0.3 Tier III
15 NC_000072.6 6 69675837 + Igk NNNGGATTATCAAGAATGGA NGG 2 0.3 Tier III
16 NC_000077.6 11 41897616 - A230004M16Rik NNNGGATTGTGAAAAATGGA NTG 2 0.0097 Tier III
Download CSV

Other clones with same target sequence:

(none)