Construct: sgRNA BRDN0001148037
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGAGCTCACGAGAAAACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK5 (341676)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76120
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
52102221 |
+ |
NEK5 |
NNNAGCTCACGAGAAAACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
217925303 |
+ |
TNS1 |
NNNAGATCAAGAGAAAACCC |
NGG |
2 |
0.8048 |
Tier II |
| 3 |
NC_000001.11 |
1 |
26854992 |
+ |
ZDHHC18 |
NNNTGGTCACGAGAAAACCC |
NGG |
2 |
0.3182 |
Tier II |
| 4 |
NC_000011.10 |
11 |
95102265 |
+ |
ENDOD1 |
NNNAGCCCAGGAGAAAACCC |
NGG |
2 |
0.2674 |
Tier II |
| 5 |
NC_000003.12 |
3 |
37485528 |
+ |
ITGA9 |
NNNAGCTCCCGAGAAAACTC |
NGG |
2 |
0.2637 |
Tier II |
| 6 |
NC_000005.10 |
5 |
150061351 |
+ |
CSF1R |
NNNAGCTCACAAGAAAACCC |
NAG |
2 |
0.2593 |
Tier II |
| 7 |
NC_000007.14 |
7 |
51312778 |
+ |
COBL |
NNNAGGTCACGAGAAAACAC |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000007.14 |
7 |
2013510 |
- |
MAD1L1 |
NNNCGCTCACGAGGAAACCC |
NGG |
2 |
0.1647 |
Tier II |
| 9 |
NC_000014.9 |
14 |
38793731 |
+ |
LINC00639 |
NNNAGCTCATGAGAAGACCC |
NGG |
2 |
0.181 |
Tier III |
| 10 |
NC_000001.11 |
1 |
246782721 |
- |
KIF28P |
NNNAGCTCACGAGCAAACCA |
NGG |
2 |
0.1071 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
40084615 |
+ |
Scin |
NNNAGCCCATGAGAAAACCC |
NGG |
2 |
0.6471 |
Tier II |
| 2 |
NC_000080.6 |
14 |
4769990 |
+ |
Gm26650 |
NNNAGCTCAGGGGAAAACCC |
NGG |
2 |
0.2809 |
Tier II |
| 3 |
NC_000080.6 |
14 |
7131040 |
- |
Gm31035 |
NNNAGCTCAGGGGAAAACCC |
NGG |
2 |
0.2809 |
Tier II |
| 4 |
NC_000071.6 |
5 |
17644900 |
+ |
Sema3c |
NNNTGCTCACCAGAAAACCC |
NGG |
2 |
0.2727 |
Tier II |
| 5 |
NC_000073.6 |
7 |
35192470 |
- |
Slc7a10 |
NNNAGCTCCCGAGGAAACCC |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000079.6 |
13 |
49720592 |
- |
Iars |
NNNAGCTCATGCGAAAACCC |
NGG |
2 |
0.2477 |
Tier II |
| 7 |
NC_000073.6 |
7 |
118431836 |
+ |
Syt17 |
NNNAGCCCACGTGAAAACCC |
NGG |
2 |
0.2292 |
Tier II |
| 8 |
NC_000070.6 |
4 |
148138208 |
+ |
Mad2l2 |
NNNAGCTAACGTGAAAACCC |
NGG |
2 |
0.2167 |
Tier II |
| 9 |
NC_000084.6 |
18 |
38955095 |
+ |
Arhgap26 |
NNNAGCTCAGGAGTAAACCC |
NGG |
2 |
0.2074 |
Tier II |
| 10 |
NC_000084.6 |
18 |
38955095 |
+ |
Gm5820 |
NNNAGCTCAGGAGTAAACCC |
NGG |
2 |
0.2074 |
Tier II |
| 11 |
NC_000081.6 |
15 |
10988358 |
- |
Amacr |
NNNAGCTCACCAGGAAACCC |
NGG |
2 |
0.2 |
Tier II |
| 12 |
NC_000079.6 |
13 |
93860495 |
- |
Arsb |
NNNAGCTCAGGAGAAAACTC |
NGG |
2 |
0.1795 |
Tier II |
| 13 |
NC_000068.7 |
2 |
32629193 |
+ |
Ak1 |
NNNAGCTCAGGAGAAAGCCC |
NGG |
2 |
0.0686 |
Tier II |
| 14 |
NC_000067.6 |
1 |
64235347 |
+ |
Gm35880 |
NNNAATTCACGAGAAAACCC |
NGG |
2 |
0.8048 |
Tier III |
| 15 |
NC_000080.6 |
14 |
5406348 |
- |
Gm41087 |
NNNAGCTCAGGGGAAAACCC |
NGG |
2 |
0.2809 |
Tier III |
| 16 |
NC_000080.6 |
14 |
5758894 |
- |
Gm36476 |
NNNAGCTCAGGGGAAAACCC |
NGG |
2 |
0.2809 |
Tier III |
| 17 |
NC_000080.6 |
14 |
6064906 |
- |
Gm51421 |
NNNAGCTCAGGGGAAAACCC |
NGG |
2 |
0.2809 |
Tier III |
| 18 |
NC_000080.6 |
14 |
7131040 |
- |
Gm41092 |
NNNAGCTCAGGGGAAAACCC |
NGG |
2 |
0.2809 |
Tier III |
| 19 |
NC_000076.6 |
10 |
24579968 |
+ |
Gm15270 |
NNNAGCTCACCAGCAAACCC |
NGG |
2 |
0.0918 |
Tier III |
Other clones with same target sequence:
(none)
