Construct: sgRNA BRDN0001148044
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGGTGTAAAGCTTGCGGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRIO (7204)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78032
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
14304498 |
+ |
TRIO |
NNNGTGTAAAGCTTGCGGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
89499006 |
+ |
NOX4 |
NNNGTATAAGGCTTGCGGTG |
NGG |
2 |
0.5556 |
Tier II |
| 3 |
NC_000010.11 |
10 |
75553483 |
+ |
LRMDA |
NNNGTGTGAAGCTTGTGGTG |
NGG |
2 |
0.2256 |
Tier II |
| 4 |
NC_000015.10 |
15 |
73887933 |
- |
TBC1D21 |
NNNGTGTCAAGCTTGTGGTG |
NGG |
2 |
0.1319 |
Tier II |
| 5 |
NC_000017.11 |
17 |
33673042 |
+ |
ASIC2 |
NNNGTGTAAAGCTTGGGATG |
NGG |
2 |
0.1065 |
Tier II |
| 6 |
NC_000011.10 |
11 |
89499006 |
+ |
H3P34 |
NNNGTATAAGGCTTGCGGTG |
NGG |
2 |
0.5556 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
42184359 |
- |
Hivep1 |
NNNCTGTAAAGCTTCCGGTG |
NGG |
2 |
0.1444 |
Tier I |
| 2 |
NC_000080.6 |
14 |
38590000 |
+ |
Nrg3 |
NNNGTGTAAAGCTTGAGGTT |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000069.6 |
3 |
74285163 |
+ |
Gm6098 |
NNNGTGTACAGCTTGAGGTG |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000079.6 |
13 |
17576122 |
+ |
Sugct |
NNNGTGTACAGCTTGAGGTG |
NGG |
2 |
0.5714 |
Tier II |
| 5 |
NC_000081.6 |
15 |
79724795 |
+ |
Sun2 |
NNNGTGGAAAGCTTGCTGTG |
NGG |
2 |
0.1471 |
Tier II |
| 6 |
NC_000073.6 |
7 |
117459149 |
+ |
Xylt1 |
NNNTTGTAAAGCTTGCTGTG |
NGG |
2 |
0.0909 |
Tier II |
| 7 |
NC_000067.6 |
1 |
191350311 |
- |
Pacc1 |
NNNGTGTAAAGCTTGCGGGG |
NGT |
2 |
0.004 |
Tier II |
| 8 |
NC_000085.6 |
19 |
56863984 |
- |
Tdrd1 |
NNNGTGCAAAGCTGGCGGTG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
