Construct: sgRNA BRDN0001148075

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTGCTCACTAGCTCATGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIKFYVE (200576)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76891

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 208326203 - PIKFYVE NNNGCTCACTAGCTCATGTG NGG 0 1.0 Tier I
2 NC_000003.12 3 68874735 + TAFA4 NNNGCTCACAAGCTCATTTG NGG 2 0.5714 Tier II
3 NC_000013.11 13 32113609 + FRY NNNGCTCACTAGCTCATCTG NGG 1 0.4762 Tier II
4 NC_000015.10 15 41581264 - TYRO3 NNNCCTCACTAGCACATGTG NGG 2 0.3277 Tier II
5 NC_000013.11 13 24319921 + C1QTNF9 NNNGCTCACTAGCTCATCTG NCG 2 0.051 Tier II
6 NC_000017.11 17 32795074 - MYO1D NNNGCTCAGTAGCTCATGTG NGA 2 0.043 Tier II
7 NC_000008.11 8 16186895 - MSR1 NNNGCTCAATAGCTCATGTG NGT 2 0.0138 Tier II
8 NC_000003.12 3 24983139 - RARB NNNGCTCACTAGCACATGTG NGT 2 0.01 Tier II
9 NC_000008.11 8 70365071 + NCOA2 NNNGCTCAGTAGCTCATGTG NGT 2 0.01 Tier II
10 NC_000001.11 1 27542056 + AHDC1 NNNGGTCACTAGCTCATGTG NGT 2 0.0097 Tier II
11 NC_000023.11 X 69180460 - LINC00269 NNNGCTCACTAGCTCATGCG NGG 1 0.2759 Tier III
12 NC_000018.10 18 70765229 - LOC105372185 NNNCCTCACTAGCTCATGTG NGT 2 0.0085 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 65246992 - Pikfyve NNNGCTCACTAGCTCATGTG NGG 0 1.0 Tier I
2 NC_000068.7 2 29698995 - Rapgef1 NNNCCTCACTAGCTCCTGTG NGG 2 0.0 Tier I
3 NC_000073.6 7 44629400 + Myh14 NNNGCTCTCTAGCTCCTGTG NGG 2 0.0 Tier I
4 NC_000080.6 14 67752480 - Dock5 NNNGCTTATTAGCTCATGTG NGG 2 0.7109 Tier II
5 NC_000076.6 10 60630945 + Cdh23 NNNGCACACTAGTTCATGTG NGG 2 0.6067 Tier II
6 NC_000068.7 2 34809983 + Fbxw2 NNNGGTCACTAACTCATGTG NGG 2 0.56 Tier II
7 NC_000070.6 4 95586906 + Fggy NNNGCTCACAACCTCATGTG NGG 2 0.4538 Tier II
8 NC_000071.6 5 114873570 - 4930519G04Rik NNNGCTGACTAGCTCACGTG NGG 2 0.3322 Tier II
9 NC_000072.6 6 33545947 + Exoc4 NNNGCTCACAAGATCATGTG NGG 2 0.3297 Tier II
10 NC_000077.6 11 109260045 - Rgs9 NNNGCTCTCTAGCTCATTTG NGG 2 0.2857 Tier II
11 NC_000071.6 5 150979195 - Kl NNNGCTCACTAGCTCATGAA NGG 2 0.2679 Tier II
12 NC_000071.6 5 121606226 - Acad12 NNNGCTCACTACCTCATGTG NAG 2 0.1373 Tier II
13 NC_000083.6 17 12161053 + Agpat4 NNNGCTCACTGGGTCATGTG NGG 2 0.0886 Tier II
14 NC_000071.6 5 114744159 - Git2 NNNGCTCACTAGGTCATGTG NAG 2 0.0354 Tier II
15 NC_000081.6 15 51801556 - Eif3h NNNGCTCACTAGCAGATGTG NGG 2 0.031 Tier II
16 NC_000078.6 12 70244878 - Trim9 NNNGCTCACTCGCTCATGTG NGT 2 0.0065 Tier II
17 NC_000070.6 4 120200509 + Gm36134 NNNGGTCACTTGCTCATGTG NGG 2 0.1846 Tier III
18 NC_000070.6 4 142657840 - Gm42340 NNNGCTCACTGGCTCATGTG NGT 2 0.0105 Tier III
19 NC_000076.6 10 92442403 - Gm40738 NNNGCTCACTTGCTCCTGTG NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)

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