Construct: sgRNA BRDN0001148075
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGCTCACTAGCTCATGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIKFYVE (200576)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76891
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
208326203 |
- |
PIKFYVE |
NNNGCTCACTAGCTCATGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
68874735 |
+ |
TAFA4 |
NNNGCTCACAAGCTCATTTG |
NGG |
2 |
0.5714 |
Tier II |
| 3 |
NC_000013.11 |
13 |
32113609 |
+ |
FRY |
NNNGCTCACTAGCTCATCTG |
NGG |
1 |
0.4762 |
Tier II |
| 4 |
NC_000015.10 |
15 |
41581264 |
- |
TYRO3 |
NNNCCTCACTAGCACATGTG |
NGG |
2 |
0.3277 |
Tier II |
| 5 |
NC_000013.11 |
13 |
24319921 |
+ |
C1QTNF9 |
NNNGCTCACTAGCTCATCTG |
NCG |
2 |
0.051 |
Tier II |
| 6 |
NC_000017.11 |
17 |
32795074 |
- |
MYO1D |
NNNGCTCAGTAGCTCATGTG |
NGA |
2 |
0.043 |
Tier II |
| 7 |
NC_000008.11 |
8 |
16186895 |
- |
MSR1 |
NNNGCTCAATAGCTCATGTG |
NGT |
2 |
0.0138 |
Tier II |
| 8 |
NC_000003.12 |
3 |
24983139 |
- |
RARB |
NNNGCTCACTAGCACATGTG |
NGT |
2 |
0.01 |
Tier II |
| 9 |
NC_000008.11 |
8 |
70365071 |
+ |
NCOA2 |
NNNGCTCAGTAGCTCATGTG |
NGT |
2 |
0.01 |
Tier II |
| 10 |
NC_000001.11 |
1 |
27542056 |
+ |
AHDC1 |
NNNGGTCACTAGCTCATGTG |
NGT |
2 |
0.0097 |
Tier II |
| 11 |
NC_000023.11 |
X |
69180460 |
- |
LINC00269 |
NNNGCTCACTAGCTCATGCG |
NGG |
1 |
0.2759 |
Tier III |
| 12 |
NC_000018.10 |
18 |
70765229 |
- |
LOC105372185 |
NNNCCTCACTAGCTCATGTG |
NGT |
2 |
0.0085 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
65246992 |
- |
Pikfyve |
NNNGCTCACTAGCTCATGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000068.7 |
2 |
29698995 |
- |
Rapgef1 |
NNNCCTCACTAGCTCCTGTG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000073.6 |
7 |
44629400 |
+ |
Myh14 |
NNNGCTCTCTAGCTCCTGTG |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000080.6 |
14 |
67752480 |
- |
Dock5 |
NNNGCTTATTAGCTCATGTG |
NGG |
2 |
0.7109 |
Tier II |
| 5 |
NC_000076.6 |
10 |
60630945 |
+ |
Cdh23 |
NNNGCACACTAGTTCATGTG |
NGG |
2 |
0.6067 |
Tier II |
| 6 |
NC_000068.7 |
2 |
34809983 |
+ |
Fbxw2 |
NNNGGTCACTAACTCATGTG |
NGG |
2 |
0.56 |
Tier II |
| 7 |
NC_000070.6 |
4 |
95586906 |
+ |
Fggy |
NNNGCTCACAACCTCATGTG |
NGG |
2 |
0.4538 |
Tier II |
| 8 |
NC_000071.6 |
5 |
114873570 |
- |
4930519G04Rik |
NNNGCTGACTAGCTCACGTG |
NGG |
2 |
0.3322 |
Tier II |
| 9 |
NC_000072.6 |
6 |
33545947 |
+ |
Exoc4 |
NNNGCTCACAAGATCATGTG |
NGG |
2 |
0.3297 |
Tier II |
| 10 |
NC_000077.6 |
11 |
109260045 |
- |
Rgs9 |
NNNGCTCTCTAGCTCATTTG |
NGG |
2 |
0.2857 |
Tier II |
| 11 |
NC_000071.6 |
5 |
150979195 |
- |
Kl |
NNNGCTCACTAGCTCATGAA |
NGG |
2 |
0.2679 |
Tier II |
| 12 |
NC_000071.6 |
5 |
121606226 |
- |
Acad12 |
NNNGCTCACTACCTCATGTG |
NAG |
2 |
0.1373 |
Tier II |
| 13 |
NC_000083.6 |
17 |
12161053 |
+ |
Agpat4 |
NNNGCTCACTGGGTCATGTG |
NGG |
2 |
0.0886 |
Tier II |
| 14 |
NC_000071.6 |
5 |
114744159 |
- |
Git2 |
NNNGCTCACTAGGTCATGTG |
NAG |
2 |
0.0354 |
Tier II |
| 15 |
NC_000081.6 |
15 |
51801556 |
- |
Eif3h |
NNNGCTCACTAGCAGATGTG |
NGG |
2 |
0.031 |
Tier II |
| 16 |
NC_000078.6 |
12 |
70244878 |
- |
Trim9 |
NNNGCTCACTCGCTCATGTG |
NGT |
2 |
0.0065 |
Tier II |
| 17 |
NC_000070.6 |
4 |
120200509 |
+ |
Gm36134 |
NNNGGTCACTTGCTCATGTG |
NGG |
2 |
0.1846 |
Tier III |
| 18 |
NC_000070.6 |
4 |
142657840 |
- |
Gm42340 |
NNNGCTCACTGGCTCATGTG |
NGT |
2 |
0.0105 |
Tier III |
| 19 |
NC_000076.6 |
10 |
92442403 |
- |
Gm40738 |
NNNGCTCACTTGCTCCTGTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
