Construct: sgRNA BRDN0001148086

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAGCCTCACAATATTGCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSK3A (2931)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76371

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 42236904 + GSK3A NNNGCCTCACAATATTGCAG NGG 0 1.0 Tier I
2 NC_000017.11 17 10672648 - MYH3 NNNGCCTAATAATATTGCAG NGG 2 0.6118 Tier II
3 NC_000017.11 17 10672648 - SCO1 NNNGCCTAATAATATTGCAG NGG 2 0.6118 Tier II
4 NC_000004.12 4 122323035 + BLTP1 NNNATCTCACAATATTGCAG NGG 2 0.5727 Tier II
5 NC_000004.12 4 44438782 - KCTD8 NNNGCCTCACAATAATGCAT NGG 2 0.4053 Tier II
6 NC_000011.10 11 72326829 + CLPB NNNGCCTCACAATTTTGAAG NGG 2 0.2872 Tier II
7 NC_000009.12 9 112507555 + KIAA1958 NNNGCCTCACAATATTGCTC NGG 2 0.2308 Tier II
8 NC_000013.11 13 35929466 - DCLK1 NNNGCCTAACAATATGGCAG NGG 2 0.225 Tier II
9 NC_000006.12 6 161395338 + PRKN NNNGCCACACAATATTCCAG NGG 2 0.2059 Tier II
10 NC_000023.11 X 52647823 + SSX7 NNNGACTCACATTATTGCAG NGG 2 0.1905 Tier II
11 NC_000012.12 12 101598788 + MYBPC1 NNNGCCTCACAATATTGCCC NGG 2 0.0887 Tier II
12 NC_000007.14 7 620194 + PRKAR1B NNNGCCTCACAATATTCCAG NTG 2 0.0092 Tier II
13 NC_000007.14 7 23227802 - LOC101927890 NNNGCCTTTCAATATTGCAG NGG 2 0.525 Tier III
14 NC_000016.10 16 51396279 + LOC102723323 NNNGCTTCACAATATTGCTG NGG 2 0.5 Tier III
15 NC_000004.12 4 55034942 - LOC124900703 NNNCCTTCACAATATTGCAG NGG 2 0.4916 Tier III
16 NC_000005.10 5 147232211 + STK32A-AS1 NNNGACTCTCAATATTGCAG NGG 2 0.3429 Tier III
17 NC_000018.10 18 26887035 - AQP4-AS1 NNNGACTCACAATATGGCAG NGG 2 0.1978 Tier III
18 NC_000012.12 12 101598788 + LOC105369938 NNNGCCTCACAATATTGCCC NGG 2 0.0887 Tier III
19 NC_000001.11 1 217929672 - LOC105372922 NNNGCCTCACTATATTGCAG NTG 2 0.012 Tier III
20 NC_000004.12 4 180456855 + LOC105377567 NNNGCCTCACAATAATGCAG NGT 2 0.0093 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 25233874 + Gsk3a NNNGCCTCACAATATTGCAG NGG 0 1.0 Tier I
2 NC_000080.6 14 34017465 - Gm5460 NNNCCATCACAATATTGCAG NGG 2 0.4916 Tier I
3 NC_000075.6 9 55869905 + Scaper NNNGCCTGACAATATTGCAG NGG 1 0.6429 Tier II
4 NC_000080.6 14 51421504 + Gm7247 NNNGCCTGACAATATTACAG NGG 2 0.6 Tier II
5 NC_000075.6 9 109700747 - Fbxw18 NNNGCCTCACAATATAGCTG NGG 2 0.4895 Tier II
6 NC_000075.6 9 72040025 + Tcf12 NNNGCCTCACATTATTGCAA NGG 2 0.3125 Tier II
7 NC_000080.6 14 117145207 - Gpc6 NNNGCCTCACAATTTTGCTG NGG 2 0.2872 Tier II
8 NC_000069.6 3 68465507 + Schip1 NNNGCCTCACTATATAGCAG NGG 2 0.2797 Tier II
9 NC_000069.6 3 68465507 + Iqschfp NNNGCCTCACTATATAGCAG NGG 2 0.2797 Tier II
10 NC_000073.6 7 133041509 + Ctbp2 NNNGCCTCACACAATTGCAG NGG 2 0.1822 Tier II
11 NC_000069.6 3 146668558 + 4930503B20Rik NNNGCCTCACATTATTGCTG NGG 2 0.1795 Tier II
12 NC_000073.6 7 35613126 + Ankrd27 NNNGCCTCCCAATATTGCAG NCG 2 0.0612 Tier II
13 NC_000071.6 5 100837929 - Abraxas1 NNNGCCTCACACTATTGGAG NGG 2 0.0351 Tier II
14 NC_000085.6 19 46642096 - Wbp1l NNNGTCTCACAATATTGCAG NGT 2 0.0103 Tier II
15 NC_000084.6 18 36965835 - Pcdha4 NNNGCCTCACAATACTGCAG NGC 2 0.0061 Tier II
16 NC_000084.6 18 36965835 - Pcdha5 NNNGCCTCACAATACTGCAG NGC 2 0.0061 Tier II
17 NC_000084.6 18 36965835 - Pcdha1 NNNGCCTCACAATACTGCAG NGC 2 0.0061 Tier II
18 NC_000084.6 18 36965835 - Pcdha3 NNNGCCTCACAATACTGCAG NGC 2 0.0061 Tier II
19 NC_000084.6 18 36965835 - Pcdha2 NNNGCCTCACAATACTGCAG NGC 2 0.0061 Tier II
20 NC_000084.6 18 36965835 - Gm37013 NNNGCCTCACAATACTGCAG NGC 2 0.0061 Tier II
21 NC_000086.7 X 136930010 - Gm6275 NNNGCCTCACAATATTGCAG NGG 0 1.0 Tier III
22 NC_000086.7 X 108435241 - Gm9218 NNNGCCTCATAATATTGCAA NGG 2 0.8824 Tier III
23 NC_000078.6 12 56667390 + Gm18339 NNNGCCAGACAATATTGCAG NGG 2 0.5625 Tier III
24 NC_000080.6 14 117145207 - Gm52112 NNNGCCTCACAATTTTGCTG NGG 2 0.2872 Tier III
25 NC_000073.6 7 83369745 + Gm29976 NNNGCCTCACCATATGGCAG NGG 2 0.1385 Tier III
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Other clones with same target sequence:

(none)