Construct: sgRNA BRDN0001148088

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCCCACTGTGTGGTCACTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO3A (53904)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76225

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 26125465 + MYO3A NNNCACTGTGTGGTCACTAG NGG 0 1.0 Tier I
2 NC_000002.12 2 170401548 + MYO3B NNNCACTGTGTGGTCACCCG NGG 2 0.0887 Tier I
3 NC_000002.12 2 205478250 + PARD3B NNNCAATGGGTGGTCACTAG NGG 2 0.5748 Tier II
4 NC_000021.9 21 21505313 - NCAM2 NNNCACTGTGTGATCACTTG NGG 2 0.497 Tier II
5 NC_000022.11 22 18921164 + PRODH NNNCACTCTGTGGACACTAG NGG 2 0.381 Tier II
6 NC_000010.11 10 69958475 - COL13A1 NNNCCCTTTGTGGTCACTAG NGG 2 0.3125 Tier II
7 NC_000023.11 X 3325166 + MXRA5 NNNCACTGTGTGGTAACTAA NGG 2 0.2083 Tier II
8 NC_000012.12 12 66356058 + GRIP1 NNNCACTGTGTGGACACGAG NGG 2 0.2063 Tier II
9 NC_000006.12 6 165623678 + PDE10A NNNCACTGTGGGGTCACCAG NGG 2 0.1714 Tier II
10 NC_000005.10 5 10235561 - ATPSCKMT NNNCACAGTGTGGTCACTAG NTG 2 0.0341 Tier II
11 NC_000020.11 20 25497238 - NINL NNNCACTGTGTGCTCACTAG NGT 2 0.0068 Tier II
12 NC_000003.12 3 42517525 - VIPR1 NNNCACAGTGTGGTCTCTAG NGG 2 0.0 Tier II
13 NC_000020.11 20 36106583 + EPB41L1 NNNCACTGTGTGGGCACCAG NGG 2 0.0 Tier II
14 NC_000002.12 2 168232068 - STK39 NNNCACTGTGTGGTCTCTGG NGG 2 0.0 Tier II
15 NC_000016.10 16 11252930 + LOC105371082 NNNCACTGAGTGGTCACTAA NGG 2 0.8705 Tier III
16 NC_000022.11 22 20305098 - PRODHLP NNNCACTCTGTGGACACTAG NGG 2 0.381 Tier III
17 NC_000010.11 10 69958475 - LOC107984242 NNNCCCTTTGTGGTCACTAG NGG 2 0.3125 Tier III
18 NC_000024.10 Y 11967891 - MXRA5Y NNNCACTGTGTGGTAACTAA NGG 2 0.2083 Tier III
19 NC_000015.10 15 87625507 - LOC102724465 NNNCACTGTCTGGTCATTAG NGG 2 0.1867 Tier III
20 NC_000015.10 15 87625507 - LOC124903580 NNNCACTGTCTGGTCATTAG NGG 2 0.1867 Tier III
21 NC_000006.12 6 73225813 - LOC122539213 NNNCACTGTGTGGTCACTGG NTG 2 0.0146 Tier III
22 NC_000005.10 5 174370841 - LINC01411 NNNCACTGTGTGGGCACAAG NGG 2 0.0 Tier III
23 NC_000002.12 2 168232068 - PHF5AP5 NNNCACTGTGTGGTCTCTGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 22399829 + Myo3a NNNCACTGTGTGGTCACTAG NGG 0 1.0 Tier I
2 NC_000068.7 2 70253038 + Myo3b NNNCACTGTGTGGTCACACG NGG 2 0.1379 Tier I
3 NC_000083.6 17 33935436 - Rgl2 NNNCACTGTGTGATCACTAG NGT 2 0.0149 Tier I
4 NC_000076.6 10 44065481 + Crybg1 NNNCACTGTGTAGTCACTTG NGG 2 0.5026 Tier II
5 NC_000082.6 16 55964733 + Gm6958 NNNCACTGTGTGGTCACTGG NGG 1 0.375 Tier II
6 NC_000071.6 5 107986319 - Dipk1a NNNCACTGTTTGGTCACCAG NGG 2 0.3482 Tier II
7 NC_000082.6 16 24901129 - Lpp NNNCACTGTGTGATCACGAG NGG 2 0.3077 Tier II
8 NC_000079.6 13 24620797 - Ripor2 NNNCACTGTGAGGTCACTGG NGG 2 0.2813 Tier II
9 NC_000074.6 8 14450888 - Dlgap2 NNNCACTCTGTGGTCACTAC NGG 2 0.2637 Tier II
10 NC_000071.6 5 34107700 + Poln NNNCACTGTGTGGTCACAGG NGG 2 0.25 Tier II
11 NC_000082.6 16 95444666 - Erg NNNCACTGTGTGGTCACCTG NGG 2 0.2308 Tier II
12 NC_000074.6 8 70817390 - Il12rb1 NNNCACTGTGGGGTCATTAG NGG 2 0.1867 Tier II
13 NC_000067.6 1 154704672 + Cacna1e NNNCACTGTCTGCTCACTAG NGG 2 0.1684 Tier II
14 NC_000067.6 1 22532133 + Rims1 NNNCACTGTGTGGACACTAG NAG 2 0.1605 Tier II
15 NC_000072.6 6 86732398 - Gmcl1 NNNCTCTGTGTTGTCACTAG NGG 2 0.1399 Tier II
16 NC_000068.7 2 10542331 - Sfmbt2 NNNCACTGTGTGGAAACTAG NGG 2 0.1376 Tier II
17 NC_000076.6 10 4009902 - Mthfd1l NNNCACTGTGTGTTCACTAC NGG 2 0.1286 Tier II
18 NC_000078.6 12 83447351 - Dpf3 NNNCACTGTGTGGTCACTAT NGC 2 0.0156 Tier II
19 NC_000076.6 10 36673667 + Hs3st5 NNNCACTGTGTGGTAACTAG NGT 2 0.0036 Tier II
20 NC_000071.6 5 150628988 + N4bp2l2 NNNCACTGTGTGGACTCTAG NGG 2 0.0 Tier II
21 NC_000083.6 17 12209644 - Agpat4 NNNCACTGTGTGGGCACCAG NGG 2 0.0 Tier II
22 NC_000077.6 11 35349294 - Slit3 NNNCACTGTGTGGGCACTAG NGT 2 0.0 Tier II
23 NC_000076.6 10 90487226 - Anks1b NNNCACTGTGTGGGCACTCG NGG 2 0.0 Tier II
24 NC_000077.6 11 69862681 - Gm32708 NNNCACTATGTGGTCACTAT NGG 2 0.7 Tier III
25 NC_000079.6 13 37431225 - Gm4035 NNNCACTGTGTGGTCACTTC NGG 2 0.2308 Tier III
26 NC_000077.6 11 37671559 - Gm12128 NNNCACTGTGTGGTAACTGG NGG 2 0.0833 Tier III
27 NC_000077.6 11 109217341 - Gm39440 NNNCACTGTGTGATCAGTAG NGG 2 0.0543 Tier III
28 NC_000080.6 14 52770386 - Tcra NNNCACTGTGTGCTCACTAG NTG 2 0.0164 Tier III
29 NC_000080.6 14 52770386 - Trav7d-4 NNNCACTGTGTGCTCACTAG NTG 2 0.0164 Tier III
30 NC_000080.6 14 53461732 - Tcra NNNCACTGTGTGCTCACTAG NTG 2 0.0164 Tier III
31 NC_000080.6 14 53461732 - Trav7-4 NNNCACTGTGTGCTCACTAG NTG 2 0.0164 Tier III
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Other clones with same target sequence:

(none)