Construct: sgRNA BRDN0001148088
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCCACTGTGTGGTCACTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MYO3A (53904)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76225
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000068.7 |
2 |
22399829 |
+ |
Myo3a |
NNNCACTGTGTGGTCACTAG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000068.7 |
2 |
70253038 |
+ |
Myo3b |
NNNCACTGTGTGGTCACACG |
NGG |
2 |
0.1379 |
Tier I |
3 |
NC_000083.6 |
17 |
33935436 |
- |
Rgl2 |
NNNCACTGTGTGATCACTAG |
NGT |
2 |
0.0149 |
Tier I |
4 |
NC_000076.6 |
10 |
44065481 |
+ |
Crybg1 |
NNNCACTGTGTAGTCACTTG |
NGG |
2 |
0.5026 |
Tier II |
5 |
NC_000082.6 |
16 |
55964733 |
+ |
Gm6958 |
NNNCACTGTGTGGTCACTGG |
NGG |
1 |
0.375 |
Tier II |
6 |
NC_000071.6 |
5 |
107986319 |
- |
Dipk1a |
NNNCACTGTTTGGTCACCAG |
NGG |
2 |
0.3482 |
Tier II |
7 |
NC_000082.6 |
16 |
24901129 |
- |
Lpp |
NNNCACTGTGTGATCACGAG |
NGG |
2 |
0.3077 |
Tier II |
8 |
NC_000079.6 |
13 |
24620797 |
- |
Ripor2 |
NNNCACTGTGAGGTCACTGG |
NGG |
2 |
0.2813 |
Tier II |
9 |
NC_000074.6 |
8 |
14450888 |
- |
Dlgap2 |
NNNCACTCTGTGGTCACTAC |
NGG |
2 |
0.2637 |
Tier II |
10 |
NC_000071.6 |
5 |
34107700 |
+ |
Poln |
NNNCACTGTGTGGTCACAGG |
NGG |
2 |
0.25 |
Tier II |
11 |
NC_000082.6 |
16 |
95444666 |
- |
Erg |
NNNCACTGTGTGGTCACCTG |
NGG |
2 |
0.2308 |
Tier II |
12 |
NC_000074.6 |
8 |
70817390 |
- |
Il12rb1 |
NNNCACTGTGGGGTCATTAG |
NGG |
2 |
0.1867 |
Tier II |
13 |
NC_000067.6 |
1 |
154704672 |
+ |
Cacna1e |
NNNCACTGTCTGCTCACTAG |
NGG |
2 |
0.1684 |
Tier II |
14 |
NC_000067.6 |
1 |
22532133 |
+ |
Rims1 |
NNNCACTGTGTGGACACTAG |
NAG |
2 |
0.1605 |
Tier II |
15 |
NC_000072.6 |
6 |
86732398 |
- |
Gmcl1 |
NNNCTCTGTGTTGTCACTAG |
NGG |
2 |
0.1399 |
Tier II |
16 |
NC_000068.7 |
2 |
10542331 |
- |
Sfmbt2 |
NNNCACTGTGTGGAAACTAG |
NGG |
2 |
0.1376 |
Tier II |
17 |
NC_000076.6 |
10 |
4009902 |
- |
Mthfd1l |
NNNCACTGTGTGTTCACTAC |
NGG |
2 |
0.1286 |
Tier II |
18 |
NC_000078.6 |
12 |
83447351 |
- |
Dpf3 |
NNNCACTGTGTGGTCACTAT |
NGC |
2 |
0.0156 |
Tier II |
19 |
NC_000076.6 |
10 |
36673667 |
+ |
Hs3st5 |
NNNCACTGTGTGGTAACTAG |
NGT |
2 |
0.0036 |
Tier II |
20 |
NC_000071.6 |
5 |
150628988 |
+ |
N4bp2l2 |
NNNCACTGTGTGGACTCTAG |
NGG |
2 |
0.0 |
Tier II |
21 |
NC_000083.6 |
17 |
12209644 |
- |
Agpat4 |
NNNCACTGTGTGGGCACCAG |
NGG |
2 |
0.0 |
Tier II |
22 |
NC_000077.6 |
11 |
35349294 |
- |
Slit3 |
NNNCACTGTGTGGGCACTAG |
NGT |
2 |
0.0 |
Tier II |
23 |
NC_000076.6 |
10 |
90487226 |
- |
Anks1b |
NNNCACTGTGTGGGCACTCG |
NGG |
2 |
0.0 |
Tier II |
24 |
NC_000077.6 |
11 |
69862681 |
- |
Gm32708 |
NNNCACTATGTGGTCACTAT |
NGG |
2 |
0.7 |
Tier III |
25 |
NC_000079.6 |
13 |
37431225 |
- |
Gm4035 |
NNNCACTGTGTGGTCACTTC |
NGG |
2 |
0.2308 |
Tier III |
26 |
NC_000077.6 |
11 |
37671559 |
- |
Gm12128 |
NNNCACTGTGTGGTAACTGG |
NGG |
2 |
0.0833 |
Tier III |
27 |
NC_000077.6 |
11 |
109217341 |
- |
Gm39440 |
NNNCACTGTGTGATCAGTAG |
NGG |
2 |
0.0543 |
Tier III |
28 |
NC_000080.6 |
14 |
52770386 |
- |
Tcra |
NNNCACTGTGTGCTCACTAG |
NTG |
2 |
0.0164 |
Tier III |
29 |
NC_000080.6 |
14 |
52770386 |
- |
Trav7d-4 |
NNNCACTGTGTGCTCACTAG |
NTG |
2 |
0.0164 |
Tier III |
30 |
NC_000080.6 |
14 |
53461732 |
- |
Tcra |
NNNCACTGTGTGCTCACTAG |
NTG |
2 |
0.0164 |
Tier III |
31 |
NC_000080.6 |
14 |
53461732 |
- |
Trav7-4 |
NNNCACTGTGTGCTCACTAG |
NTG |
2 |
0.0164 |
Tier III |
Other clones with same target sequence:
(none)