Construct: sgRNA BRDN0001148089
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTTGTGAATGACGTACTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- INSR (3643)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75507
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
7184382 |
+ |
INSR |
NNNTGTGAATGACGTACTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
51121768 |
- |
PRKG1 |
NNNTGTGAATGATGTACTGA |
NGG |
2 |
0.6563 |
Tier II |
| 3 |
NC_000015.10 |
15 |
40559647 |
- |
CCDC32 |
NNNTGTTAATGATGTACTGG |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000011.10 |
11 |
106691944 |
+ |
GUCY1A2 |
NNNTCTGAATGACTTACTGG |
NGG |
2 |
0.2095 |
Tier II |
| 5 |
NC_000010.11 |
10 |
48252108 |
- |
FRMPD2 |
NNNTGTGAATCACGTACTGC |
NGG |
2 |
0.1837 |
Tier II |
| 6 |
NC_000006.12 |
6 |
93369632 |
- |
EPHA7 |
NNNTGTGAATGACATAGTGG |
NGG |
2 |
0.0441 |
Tier II |
| 7 |
NC_000014.9 |
14 |
89847338 |
- |
EFCAB11 |
NNNTTTGAATGAGGTACTGG |
NGG |
2 |
0.0409 |
Tier II |
| 8 |
NC_000003.12 |
3 |
154138967 |
+ |
ARHGEF26 |
NNNTGTGAATGACTTACTGG |
NGC |
2 |
0.0059 |
Tier II |
| 9 |
NC_000019.10 |
19 |
37550127 |
- |
LOC124904708 |
NNNTGTGAAGGACGTTCTGG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000003.12 |
3 |
150985305 |
+ |
CLRN1-AS1 |
NNNTGTAAATGACGAACTGG |
NGG |
2 |
0.5789 |
Tier III |
| 11 |
NC_000006.12 |
6 |
47662184 |
+ |
ADGRF2P |
NNNTGTGAATGACGTAGTGC |
NGG |
2 |
0.0252 |
Tier III |
| 12 |
NC_000019.10 |
19 |
37550127 |
- |
ZNF571-AS1 |
NNNTGTGAAGGACGTTCTGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
3211445 |
+ |
Insr |
NNNTGTGAATGACGTATTGG |
NGG |
1 |
0.4667 |
Tier I |
| 2 |
NC_000068.7 |
2 |
33228240 |
+ |
Angptl2 |
NNNTGTGTATGACCTACTGG |
NGG |
2 |
0.1837 |
Tier I |
| 3 |
NC_000072.6 |
6 |
52901243 |
- |
Jazf1 |
NNNTGTGAATGACTTACTGT |
NGG |
2 |
0.1867 |
Tier II |
| 4 |
NC_000068.7 |
2 |
33228240 |
+ |
Ralgps1 |
NNNTGTGTATGACCTACTGG |
NGG |
2 |
0.1837 |
Tier II |
| 5 |
NC_000074.6 |
8 |
64815532 |
- |
Klhl2 |
NNNTGTGAATGATGGACTGG |
NGG |
2 |
0.035 |
Tier II |
| 6 |
NC_000068.7 |
2 |
154566854 |
+ |
E2f1 |
NNNTGTGAATGACGTACTGT |
NTG |
2 |
0.0273 |
Tier II |
| 7 |
NC_000076.6 |
10 |
107319049 |
+ |
Lin7a |
NNNTGTGAATGACGTAATGG |
NGC |
2 |
0.0104 |
Tier II |
| 8 |
NC_000076.6 |
10 |
42818816 |
- |
Sec63 |
NNNTGTGAATGACTTCCTGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000071.6 |
5 |
92996687 |
+ |
Gm36764 |
NNNTGTTAATGAGGTACTGG |
NGG |
2 |
0.0779 |
Tier III |
Other clones with same target sequence:
(none)
