Construct: sgRNA BRDN0001148098

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGCTGTACCAAATGTTGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK10 (5602)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75983

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 86101937 - MAPK10 NNNCTGTACCAAATGTTGTG NGG 0 1.0 Tier I
2 NC_000022.11 22 25788587 + MYO18B NNNCTGTACCAAATATTGTG NGG 1 0.9412 Tier II
3 NC_000002.12 2 164802256 - COBLL1 NNNTTGTGCCAAATGTTGTG NGG 2 0.5867 Tier II
4 NC_000007.14 7 158324280 - PTPRN2 NNNCTGTACCAGATGCTGTG NGG 2 0.4815 Tier II
5 NC_000005.10 5 145966621 + SH3RF2 NNNCTGCACCAAATGCTGTG NGG 2 0.4583 Tier II
6 NC_000003.12 3 197861471 + LRCH3 NNNCTGTGCCAAAAGTTGTG NGG 2 0.454 Tier II
7 NC_000002.12 2 225472472 - NYAP2 NNNCTGTACCAAATGCTGTC NGG 2 0.2857 Tier II
8 NC_000009.12 9 136664950 + EGFL7 NNNCTGTAACAATTGTTGTG NGG 2 0.2571 Tier II
9 NC_000020.11 20 63777590 - ZBTB46 NNNCTGTACCTGATGTTGTG NGG 2 0.2222 Tier II
10 NC_000002.12 2 72756353 - EXOC6B NNNCTGTCCCACATGTTGTG NGG 2 0.1128 Tier II
11 NC_000007.14 7 153777989 - DPP6 NNNCTGTCCCACATGTTGTG NGG 2 0.1128 Tier II
12 NC_000003.12 3 40389763 + ENTPD3 NNNCTGTAGCAAATGTTGTG NTG 2 0.0241 Tier II
13 NC_000003.12 3 49640328 + BSN NNNCTGTACCACATGTTGTG NGC 2 0.0058 Tier II
14 NC_000007.14 7 110394559 + LOC105375451 NNNCTGTACCAAGTGTTGTG NGG 1 0.6522 Tier III
15 NC_000011.10 11 113589435 + LOC107984390 NNNCTGCACTAAATGTTGTG NGG 2 0.6471 Tier III
16 NC_000001.11 1 166309494 - LOC112268276 NNNCAATACCAAATGTTGTG NGG 2 0.5 Tier III
17 NC_000005.10 5 145966621 + LOC107986458 NNNCTGCACCAAATGCTGTG NGG 2 0.4583 Tier III
18 NC_000014.9 14 101559076 + DIO3OS NNNCTGCACCAAATGCTGTG NGG 2 0.4583 Tier III
19 NC_000003.12 3 146181865 - LOC105374145 NNNCTGTACTAAATTTTGTG NGG 2 0.1345 Tier III
20 NC_000005.10 5 128832764 - LOC105379168 NNNCTGTACCAAATGCTGTG NGC 2 0.0148 Tier III
21 NC_000002.12 2 38235645 - LOC102723739 NNNCTGTACCAAATGTTTTG NGT 2 0.0108 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 102990547 - Mapk10 NNNCTGTACCAGATGCTGTG NGG 2 0.4815 Tier I
2 NC_000071.6 5 19737889 + Magi2 NNNCTGTACAAAATGATGTG NGG 2 0.7879 Tier II
3 NC_000072.6 6 96503715 + Tafa1 NNNCTGTACCAAATGTTTTA NGG 2 0.625 Tier II
4 NC_000070.6 4 9286780 - Clvs1 NNNCTGTATCGAATGTTGTG NGG 2 0.5688 Tier II
5 NC_000069.6 3 136820409 + Ppp3ca NNNCTGTAGCAAATGATGTG NGG 2 0.5628 Tier II
6 NC_000081.6 15 78451416 - Tmprss6 NNNCTGTGCCAAGTGTTGTG NGG 2 0.4783 Tier II
7 NC_000072.6 6 34314136 - Akr1b3 NNNCTGTGGCAAATGTTGTG NGG 2 0.454 Tier II
8 NC_000078.6 12 57185580 + Slc25a21 NNNCTGTACCAAATGCTTTG NGG 2 0.4444 Tier II
9 NC_000074.6 8 8630614 + Efnb2 NNNCTGTACCAAAAGTTTTG NGG 2 0.4127 Tier II
10 NC_000070.6 4 155400454 - Cfap74 NNNCTGTAGCAAGTGTTGTG NGG 2 0.4037 Tier II
11 NC_000068.7 2 41883217 + Lrp1b NNNCTGTACCAAATCTTGTA NGG 2 0.2557 Tier II
12 NC_000083.6 17 90218802 - Nrxn1 NNNCTGTACGAAGTGTTGTG NGG 2 0.2536 Tier II
13 NC_000072.6 6 96414700 + Tafa1 NNNCTGTACCACATGTTTTG NGG 2 0.1754 Tier II
14 NC_000084.6 18 32213391 + Map3k2 NNNCTGTAACAAATTTTGTG NGG 2 0.1224 Tier II
15 NC_000079.6 13 48832684 + Phf2 NNNCTGTACCAAATGTTTTG NTG 2 0.026 Tier II
16 NC_000070.6 4 134112851 + Ubxn11 NNNCTGTACCAAAGGTTGGG NGG 2 0.0 Tier II
17 NC_000071.6 5 19737889 + Gm52766 NNNCTGTACAAAATGATGTG NGG 2 0.7879 Tier III
18 NC_000070.6 4 9286780 - Gm11816 NNNCTGTATCGAATGTTGTG NGG 2 0.5688 Tier III
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Other clones with same target sequence:

(none)