Construct: sgRNA BRDN0001148101
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCTAGTCTCGGTAAGAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80212
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
71140928 |
- |
MED12 |
NNNTAGTCTCGGTAACTGTG |
NGG |
2 |
0.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
112390219 |
- |
ALPK1 |
NNNTAATCCCGGTAAGAGTG |
NGG |
2 |
0.9231 |
Tier II |
| 3 |
NC_000008.11 |
8 |
3731407 |
+ |
CSMD1 |
NNNTTGTCTTGGTAAGAGTG |
NGG |
2 |
0.3422 |
Tier II |
| 4 |
NC_000012.12 |
12 |
4657562 |
+ |
NDUFA9 |
NNNTTGTCTCGGAAAGAGTG |
NGG |
2 |
0.2517 |
Tier II |
| 5 |
NC_000012.12 |
12 |
24488743 |
- |
SOX5 |
NNNGAGTCTGGGTAAGAGTG |
NGG |
2 |
0.2431 |
Tier II |
| 6 |
NC_000001.11 |
1 |
25479030 |
+ |
MACO1 |
NNNTAGTCTAGGGAAGAGTG |
NGG |
2 |
0.2261 |
Tier II |
| 7 |
NC_000002.12 |
2 |
191934286 |
+ |
CAVIN2-AS1 |
NNNTAGTCTAGGTAAGAGTT |
NGG |
2 |
0.6067 |
Tier III |
| 8 |
NC_000005.10 |
5 |
73463163 |
+ |
LINC01386 |
NNNTAGTCTGGGTAAGAGTA |
NGG |
2 |
0.3646 |
Tier III |
| 9 |
NC_000004.12 |
4 |
151922982 |
+ |
LOC127898556 |
NNNTAGCCTCGGTATGAGTG |
NGG |
2 |
0.1375 |
Tier III |
| 10 |
NC_000004.12 |
4 |
151922982 |
+ |
LOC127898557 |
NNNTAGCCTCGGTATGAGTG |
NGG |
2 |
0.1375 |
Tier III |
| 11 |
NC_000003.12 |
3 |
192030497 |
- |
LOC105374277 |
NNNTAGTCTGGGTAACAGTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
105689428 |
+ |
Carmil2 |
NNNTAGGCACGGTAAGAGTG |
NGG |
2 |
0.5462 |
Tier I |
| 2 |
NC_000071.6 |
5 |
66927125 |
+ |
Limch1 |
NNNGAGTCTCAGTAAGAGTG |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000085.6 |
19 |
3474673 |
- |
Ppp6r3 |
NNNTTGTCTCGGTGAGAGTG |
NGG |
2 |
0.1697 |
Tier II |
| 4 |
NC_000083.6 |
17 |
70621066 |
- |
Dlgap1 |
NNNTTGTCTCTGTAAGAGTG |
NGG |
2 |
0.1399 |
Tier II |
Other clones with same target sequence:
(none)
