Construct: sgRNA BRDN0001148134

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTAGCCCTGATAACCCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WNK3 (65267)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76926

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 54255834 - WNK3 NNNAGCCCTGATAACCCAAG NGG 0 1.0 Tier I
2 NC_000009.12 9 101249391 - PLPPR1 NNNACCCCTGATAACACAAG NGG 2 0.7857 Tier II
3 NC_000003.12 3 85757004 - CADM2 NNNAGCTCTGACAACCCAAG NGG 2 0.7697 Tier II
4 NC_000001.11 1 18204521 - IGSF21 NNNAGCCATGGTAACCCAAG NGG 2 0.4225 Tier II
5 NC_000003.12 3 128368866 + EEFSEC NNNAGGCCTGAAAACCCAAG NGG 2 0.4 Tier II
6 NC_000001.11 1 202952665 - ADIPOR1 NNNAGGCCTGAGAACCCAAG NGG 2 0.25 Tier II
7 NC_000002.12 2 124494289 + CNTNAP5 NNNAGGCCTGAGAACCCAAG NGG 2 0.25 Tier II
8 NC_000014.9 14 37064244 - SLC25A21 NNNAGGCCTGAGAACCCAAG NGG 2 0.25 Tier II
9 NC_000016.10 16 20922925 + LYRM1 NNNAGGCCTGAGAACCCAAG NGG 2 0.25 Tier II
10 NC_000016.10 16 85914584 + IRF8 NNNAGCCCAGATAACCCAAG NAG 2 0.2407 Tier II
11 NC_000021.9 21 38476609 + ERG NNNAGCCCTGATAACCCATC NGG 2 0.2308 Tier II
12 NC_000011.10 11 108736413 - DDX10 NNNATCCATGATAACCCAAG NGG 2 0.195 Tier II
13 NC_000011.10 11 99981308 + CNTN5 NNNAGGCCTGATAACCCAGG NGG 2 0.1875 Tier II
14 NC_000002.12 2 168820324 - NOSTRIN NNNATCCCTGATAACCCATG NGG 2 0.1615 Tier II
15 NC_000014.9 14 74155415 - LIN52 NNNAGCCCTGATTTCCCAAG NGG 2 0.16 Tier II
16 NC_000012.12 12 12940709 - GPRC5D NNNAGCCCTGATAGCCCACG NGG 2 0.0966 Tier II
17 NC_000022.11 22 21551543 + UBE2L3 NNNAGCCCTGATCACCCAAC NGG 2 0.0902 Tier II
18 NC_000006.12 6 121185350 - TBC1D32 NNNAGCCCTGATAACACAAG NGA 2 0.0694 Tier II
19 NC_000013.11 13 29513879 + SLC7A1 NNNAGCCCTGAGAAGCCAAG NGG 2 0.025 Tier II
20 NC_000006.12 6 163301144 - PACRG NNNAGCCCTGATAAGCCAAC NGG 2 0.0214 Tier II
21 NC_000011.10 11 97798283 + LOC105369453 NNNAGGCCTGAGAACCCAAG NGG 2 0.25 Tier III
22 NC_000011.10 11 108736413 - LOC124902750 NNNATCCATGATAACCCAAG NGG 2 0.195 Tier III
23 NC_000012.12 12 12940709 - GPRC5D-AS1 NNNAGCCCTGATAGCCCACG NGG 2 0.0966 Tier III
24 NC_000020.11 20 50292965 - LINC01270 NNNAGCCCTGATCACCCAAG NCG 2 0.0226 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 94548416 - Cpne2 NNNAGCACTGAGAACCCAAG NGG 2 0.375 Tier I
2 NC_000086.7 X 151274972 + Wnk3 NNNAACCCTGATACCCCAAG NGG 2 0.1857 Tier I
3 NC_000067.6 1 43549492 + Nck2 NNNAGCCCTAATAACCCAGG NGG 2 0.35 Tier II
4 NC_000068.7 2 40680294 - Lrp1b NNNATCCCTGATAACACAAG NGG 2 0.3 Tier II
5 NC_000072.6 6 48428344 + Zfp467 NNNAGCCCTGAGAACCCATG NGG 2 0.2692 Tier II
6 NC_000078.6 12 55110429 - Srp54a NNNAGCCCTGAGAGCCCAAG NGG 2 0.2333 Tier II
7 NC_000078.6 12 55185489 - Srp54b NNNAGCCCTGAGAGCCCAAG NGG 2 0.2333 Tier II
8 NC_000082.6 16 95439409 + Erg NNNAGCCCTGATAACCCATC NGG 2 0.2308 Tier II
9 NC_000073.6 7 105712417 - Dnhd1 NNNAGTCCTGATCACCCAAG NGG 2 0.1955 Tier II
10 NC_000071.6 5 73194174 + Fryl NNNACCCCTGATAAACCAAG NGG 2 0.1746 Tier II
11 NC_000074.6 8 106850507 - Tango6 NNNAGCCCTGAACACCCAAG NGG 2 0.1684 Tier II
12 NC_000078.6 12 84501006 - Lin52 NNNAGCCCTGATTTCCCAAG NGG 2 0.16 Tier II
13 NC_000071.6 5 33656489 + Tmem129 NNNAGCCCTGTTAACCCAAC NGG 2 0.1319 Tier II
14 NC_000080.6 14 23718054 - Kcnma1 NNNATCCCTGTTAACCCAAG NGG 2 0.0923 Tier II
15 NC_000080.6 14 23348800 - Kcnma1 NNNAGCCCTGAGAACGCAAG NGG 2 0.0769 Tier II
16 NC_000067.6 1 61729780 + Pard3b NNNAGCCCTGATAATCCAAA NGG 2 0.0625 Tier II
17 NC_000086.7 X 107400809 + Itm2a NNNAGCCCTGATAACCCAAG NTG 1 0.039 Tier II
18 NC_000073.6 7 74377395 + Slco3a1 NNNAGCCCTGATAACCCAAA NTG 2 0.0365 Tier II
19 NC_000067.6 1 162160790 - Dnm3 NNNAGCCCTGATAAGCCAAC NGG 2 0.0214 Tier II
20 NC_000073.6 7 98452337 - Gucy2d NNNAGCCCTGATCATCCAAG NGG 2 0.014 Tier II
21 NC_000074.6 8 3734241 - Gm16553 NNNAGCACTGATAACCCATG NGG 2 0.4038 Tier III
22 NC_000078.6 12 28998373 - Gm31508 NNNAGCCCTCATATCCCAAG NGG 2 0.2133 Tier III
23 NC_000077.6 11 6086411 + Gm46269 NNNAGCCCTGAGACCCCAAG NGG 2 0.1071 Tier III
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Other clones with same target sequence:

(none)