Construct: sgRNA BRDN0001148152

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGTGAGTGAAGATGGACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCYL3 (57147)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76792

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 169876035 - SCYL3 NNNTGAGTGAAGATGGACAC NGG 0 1.0 Tier I
2 NC_000012.12 12 132145173 + NOC4L NNNTGAGTGTAGATGAACAC NGG 2 0.8824 Tier II
3 NC_000002.12 2 86557091 - CHMP3 NNNTGAGAGAAGATAGACAC NGG 2 0.7529 Tier II
4 NC_000002.12 2 86557091 - RNF103-CHMP3 NNNTGAGAGAAGATAGACAC NGG 2 0.7529 Tier II
5 NC_000014.9 14 99748984 - EML1 NNNTGAATGAAGATGGATAC NGG 2 0.6429 Tier II
6 NC_000002.12 2 201858086 - CDK15 NNNTGGGTGTAGATGGACAC NGG 2 0.6303 Tier II
7 NC_000002.12 2 2212905 - MYT1L NNNTCAGAGAAGATGGACAC NGG 2 0.6286 Tier II
8 NC_000012.12 12 71623184 - ZFC3H1 NNNTCAGAGAAGATGGACAC NGG 2 0.6286 Tier II
9 NC_000019.10 19 35338475 + CD22 NNNGGAGAGAAGATGGACAC NGG 2 0.5 Tier II
10 NC_000008.11 8 132701402 + DNAAF11 NNNTGAGGAAAGATGGACAC NGG 2 0.4714 Tier II
11 NC_000008.11 8 17350639 - MTMR7 NNNTGGGTGGAGATGGACAC NGG 2 0.3968 Tier II
12 NC_000001.11 1 213746704 + RPS6KC1 NNNAGAGAGAAGATGGACAC NGG 2 0.381 Tier II
13 NC_000015.10 15 33084824 - FMN1 NNNTGAGTCAGGATGGACAC NGG 2 0.35 Tier II
14 NC_000023.11 X 155045671 + FUNDC2 NNNTGAGAGAATATGGACAC NGG 2 0.3077 Tier II
15 NC_000016.10 16 69604714 - NFAT5 NNNAGATTGAAGATGGACAC NGG 2 0.2721 Tier II
16 NC_000013.11 13 48707896 - CYSLTR2 NNNTGAATGAAGATGGACAC NAG 2 0.2593 Tier II
17 NC_000012.12 12 120662105 - CABP1 NNNTGGGTGCAGATGGACAC NGG 2 0.2381 Tier II
18 NC_000010.11 10 333358 - DIP2C NNNTGACTGCAGATGGACAC NGG 2 0.2292 Tier II
19 NC_000005.10 5 62624495 + IPO11 NNNTGAGTGAAGATGACCAC NGG 2 0.1765 Tier II
20 NC_000015.10 15 41090219 + INO80 NNNTGAGAGAAGCTGGACAC NGG 2 0.1684 Tier II
21 NC_000018.10 18 79819964 - KCNG2 NNNTGAGAGAAGCTGGACAC NGG 2 0.1684 Tier II
22 NC_000001.11 1 110613311 - KCNA2 NNNTGCGTGCAGATGGACAC NGG 2 0.1515 Tier II
23 NC_000003.12 3 85836458 - CADM2 NNNTGATTGAAGATGGAGAC NGG 2 0.0762 Tier II
24 NC_000014.9 14 77554890 - SPTLC2 NNNTGAGTGAACATGGAGAC NGG 2 0.0706 Tier II
25 NC_000001.11 1 236171416 + GPR137B NNNTGAGTGAAGATGGGCAT NGG 2 0.0529 Tier II
26 NC_000002.12 2 28405489 - FOSL2 NNNAGAGTGAAGATGGACAG NGG 2 0.028 Tier II
27 NC_000001.11 1 149917124 - SV2A NNNTGAGTGAAGGTGGACAC NTG 2 0.0254 Tier II
28 NC_000013.11 13 100670332 + TMTC4 NNNTGAGTGAAGATGGAGAC NGA 2 0.0093 Tier II
29 NC_000006.12 6 133344100 + EYA4 NNNTGAGTGAAGATGGAGAC NGC 2 0.003 Tier II
30 NC_000009.12 9 36856390 + PAX5 NNNTGAGTGAAAAGGGACAC NGG 2 0.0 Tier II
31 NC_000011.10 11 57607271 - SERPING1 NNNTGAGTGAAGAGGGATAC NGG 2 0.0 Tier II
32 NC_000005.10 5 5309066 - ADAMTS16 NNNTGAGTGAATATGCACAC NGG 2 0.0 Tier II
33 NC_000023.11 X 6848093 - PUDP NNNTTAGTGAAGATGTACAC NGG 2 0.0 Tier II
34 NC_000005.10 5 37920169 - LINC02117 NNNTGAGTGAGAATGGACAC NGG 2 0.6067 Tier III
35 NC_000002.12 2 2735608 + LOC105373390 NNNTCAGGGAAGATGGACAC NGG 2 0.5762 Tier III
36 NC_000002.12 2 2735861 + LOC105373390 NNNTCAGGGAAGATGGACAC NGG 2 0.5762 Tier III
37 NC_000008.11 8 17350639 - LOC102724838 NNNTGGGTGGAGATGGACAC NGG 2 0.3968 Tier III
38 NC_000001.11 1 106841046 + LOC105378888 NNNAGAGTGAAGAAGGACAC NGG 2 0.2948 Tier III
39 NC_000002.12 2 2729542 + LOC105373390 NNNTGAGTGATGGTGGACAC NGG 2 0.2007 Tier III
40 NC_000001.11 1 160778865 - LOC105371470 NNNTGAGTGAGGATGGACAT NGG 2 0.195 Tier III
41 NC_000005.10 5 62624495 + IPO11-LRRC70 NNNTGAGTGAAGATGACCAC NGG 2 0.1765 Tier III
42 NC_000020.11 20 52423918 - LOC105372666 NNNTGAGTGAAGGTGGGCAC NGG 2 0.1151 Tier III
43 NC_000020.11 20 52424188 - LOC105372666 NNNTGAGTGAAGGTGGGCAC NGG 2 0.1151 Tier III
44 NC_000020.11 20 52424248 - LOC105372666 NNNTGAGTGAAGGTGGGCAC NGG 2 0.1151 Tier III
45 NC_000008.11 8 57467190 - LOC105375855 NNNTGAATGAAGATGGACAC NGA 2 0.0694 Tier III
46 NC_000003.12 3 999191 - LOC107986059 NNNTGAGTGAAGTTGGACAC NGA 2 0.0208 Tier III
47 NC_000005.10 5 5309066 - LOC101929200 NNNTGAGTGAATATGCACAC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 163936475 + Scyl3 NNNTGAGTGAGGATGGGCAC NGG 2 0.1147 Tier I
2 NC_000068.7 2 82950866 - Fsip2 NNNTGAGTGAGGATGAACAC NGG 2 0.65 Tier II
3 NC_000080.6 14 122328378 - Clybl NNNTGTGGGAAGATGGACAC NGG 2 0.5238 Tier II
4 NC_000072.6 6 141295128 - Pde3a NNNTGAGAAAAGATGGACAC NGG 2 0.5143 Tier II
5 NC_000077.6 11 46336774 + Itk NNNTGAATGAAGATGGACAA NGG 2 0.5 Tier II
6 NC_000077.6 11 118200324 + Cyth1 NNNTGAGGGAAGGTGGACAC NGG 2 0.4783 Tier II
7 NC_000068.7 2 41645411 + Lrp1b NNNTGATGGAAGATGGACAC NGG 2 0.419 Tier II
8 NC_000071.6 5 141856950 + Sdk1 NNNTCAGTGAAGATGGACAA NGG 2 0.3929 Tier II
9 NC_000074.6 8 39553231 - Msr1 NNNTGAGTGAAGAAGGACTC NGG 2 0.3333 Tier II
10 NC_000068.7 2 54965163 + Galnt13 NNNTGAGTGAAGATGGAATC NGG 2 0.2899 Tier II
11 NC_000073.6 7 84138121 + Abhd17c NNNTGAGTGAAGATGGATGC NGG 2 0.2411 Tier II
12 NC_000073.6 7 102087484 - Trpc2 NNNTGAGAGAAGATGGACAC NAG 2 0.2074 Tier II
13 NC_000073.6 7 102087484 - Xntrpc NNNTGAGAGAAGATGGACAC NAG 2 0.2074 Tier II
14 NC_000069.6 3 26868207 - Spata16 NNNTGGGTGAAGATGGACAC NAG 2 0.1852 Tier II
15 NC_000077.6 11 86649051 + Vmp1 NNNTGATTGATGATGGACAC NGG 2 0.1758 Tier II
16 NC_000082.6 16 96836468 - Dscam NNNTCAGTGAAGCTGGACAC NGG 2 0.1654 Tier II
17 NC_000074.6 8 66328253 - Marchf1 NNNTGAGTGAAGATGGGAAC NGG 2 0.095 Tier II
18 NC_000082.6 16 30972454 - Xxylt1 NNNTGAGTGATGATGGGCAC NGG 2 0.0543 Tier II
19 NC_000077.6 11 53650374 - Rad50 NNNTGAGTGAAGATGAACAC NGT 2 0.0161 Tier II
20 NC_000083.6 17 71203932 - Lpin2 NNNTGAGTGAAGATGGCCAC NTG 2 0.0069 Tier II
21 NC_000086.7 X 138013429 - Il1rapl2 NNNTGAGTGAAGATGGACAG NGC 2 0.0013 Tier II
22 NC_000074.6 8 82031625 + Inpp4b NNNAGAGTGAAGAGGGACAC NGG 2 0.0 Tier II
23 NC_000082.6 16 46066436 - Cd96 NNNTGAGTGAAGAGGGACAC NGA 2 0.0 Tier II
24 NC_000075.6 9 117071083 + Rbms3 NNNTGAGTGAAGATGTCCAC NGG 2 0.0 Tier II
25 NC_000079.6 13 100196454 + Naip2 NNNTGAGTGCAGATGCACAC NGG 2 0.0 Tier II
26 NC_000072.6 6 55584819 + Gm34441 NNNTCAGTGTAGATGGACAC NGG 2 0.6933 Tier III
27 NC_000073.6 7 43990050 + Klk1b10-ps NNNTGAGTGAGGGTGGACAC NGG 2 0.4239 Tier III
28 NC_000077.6 11 34025913 + 4930469K13Rik NNNTGAGTAAGGATGGACAC NGG 2 0.4179 Tier III
29 NC_000069.6 3 26868207 - Gm32458 NNNTGGGTGAAGATGGACAC NAG 2 0.1852 Tier III
30 NC_000082.6 16 30972454 - Gm15742 NNNTGAGTGATGATGGGCAC NGG 2 0.0543 Tier III
31 NC_000080.6 14 54180180 + Tcra NNNTGTGTGAAGATGGACAC NTG 2 0.0278 Tier III
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Other clones with same target sequence:

(none)