Construct: sgRNA BRDN0001148155

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGGACTTTCTGCATAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MKNK2 (2872)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77765

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 2042771 - MKNK2 NNNGACTTTCTGCATAACAA NGG 0 1.0 Tier I
2 NC_000001.11 1 46572068 - MKNK1 NNNGACTTCCTGCATACCAA NGG 2 0.1629 Tier I
3 NC_000021.9 21 29321272 - BACH1 NNNGACATTCTGCATAACAA NTG 2 0.0341 Tier I
4 NC_000002.12 2 70959091 + ATP6V1B1 NNNGACTTTCTGGATATCAA NGG 2 0.0182 Tier I
5 NC_000010.11 10 30340239 + MTPAP NNNCACTTTCTGCATAACAA NGT 2 0.0085 Tier I
6 NC_000003.12 3 175236175 + NAALADL2 NNNGACTTACTGCATAATAA NGG 2 0.5969 Tier II
7 NC_000006.12 6 87568537 - RARS2 NNNGACATTCTGCATAATAA NGG 2 0.5625 Tier II
8 NC_000004.12 4 142189044 - INPP4B NNNGACTTTTTGCATAAAAA NGG 2 0.5068 Tier II
9 NC_000001.11 1 100228267 + DBT NNNGACATTCTGCAAAACAA NGG 2 0.5066 Tier II
10 NC_000005.10 5 77337006 - PDE8B NNNCACTTCCTGCATAACAA NGG 2 0.4887 Tier II
11 NC_000012.12 12 66631672 + GRIP1 NNNGACTTTCTGTGTAACAA NGG 2 0.3267 Tier II
12 NC_000004.12 4 61935038 - ADGRL3 NNNGACCTTCTGAATAACAA NGG 2 0.2644 Tier II
13 NC_000002.12 2 24314723 + ITSN2 NNNGTCTTTCTGCAAAACAA NGG 2 0.2105 Tier II
14 NC_000005.10 5 83289825 - XRCC4 NNNGTCTTTCTCCATAACAA NGG 2 0.1925 Tier II
15 NC_000017.11 17 975595 - NXN NNNGACTTCCTGCATGACAA NGG 2 0.1775 Tier II
16 NC_000005.10 5 128393421 + FBN2 NNNGTCTTTCTGCATAACAA NAG 2 0.0943 Tier II
17 NC_000013.11 13 96261663 - HS6ST3 NNNGACTTTCTGTATAAGAA NGG 2 0.0933 Tier II
18 NC_000017.11 17 18302110 + TOP3A NNNGAGTTTCTGGATAACAA NGG 2 0.0682 Tier II
19 NC_000007.14 7 132422249 + PLXNA4 NNNGACTTCCTGCAGAACAA NGG 2 0.0462 Tier II
20 NC_000008.11 8 35692895 + UNC5D NNNGCCTTTCTGCATAACAA NTG 2 0.0195 Tier II
21 NC_000012.12 12 21343924 - SLCO1A2 NNNTACTTTCTGCATAACAA NTG 2 0.0142 Tier II
22 NC_000003.12 3 175236175 + NAALADL2-AS2 NNNGACTTACTGCATAATAA NGG 2 0.5969 Tier III
23 NC_000007.14 7 3281169 - SDK1-AS1 NNNTACTTTTTGCATAACAA NGG 2 0.3422 Tier III
24 NC_000004.12 4 127609873 - LOC105377412 NNNGTCTTTCTCCATAACAA NGG 2 0.1925 Tier III
25 NC_000016.10 16 48643438 - LOC105371240 NNNGAGTTTCTGCATAGCAA NGG 2 0.0882 Tier III
26 NC_000001.11 1 192595240 + LOC105371664 NNNGACGTTCTGCATAAGAA NGG 2 0.0784 Tier III
27 NC_000008.11 8 35692895 + LOC101929550 NNNGCCTTTCTGCATAACAA NTG 2 0.0195 Tier III
28 NC_000014.9 14 22308471 - TRA NNNCACTTTCTGCATTACAA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 80669303 - Mknk2 NNNGACTTCCTGCATAACAA NGG 1 0.9231 Tier I
2 NC_000074.6 8 121801223 + Klhdc4 NNNGACTCTCTGCAAAACAA NGG 2 0.5789 Tier I
3 NC_000067.6 1 107539329 + Serpinb10 NNNGACATTCTGCATAACAA NGG 1 0.875 Tier II
4 NC_000068.7 2 127807261 + Bub1 NNNGACTTTCTGTATAACAA NGG 1 0.7 Tier II
5 NC_000085.6 19 41976600 + Mms19 NNNGATTTTCTGTATAACAA NGG 2 0.65 Tier II
6 NC_000078.6 12 4300406 + Ncoa1 NNNCACTTCCTGCATAACAA NGG 2 0.4887 Tier II
7 NC_000084.6 18 84529443 + Zfp407 NNNCACTATCTGCATAACAA NGG 2 0.4235 Tier II
8 NC_000073.6 7 99024716 + Uvrag NNNGACTTTCTGAATAATAA NGG 2 0.2473 Tier II
9 NC_000070.6 4 87925848 - Mllt3 NNNGATTTTCTGCATAACCA NGG 2 0.1921 Tier II
10 NC_000086.7 X 152055021 + Smc1a NNNGACTTTCTGCACAACAT NGG 2 0.1636 Tier II
11 NC_000084.6 18 6059051 + Arhgap12 NNNGACTTGCTGCATAACAC NGG 2 0.1407 Tier II
12 NC_000068.7 2 68356411 - Stk39 NNNGACTTTCTGCCTAATAA NGG 2 0.1378 Tier II
13 NC_000068.7 2 179792331 - Cdh4 NNNCACTTTCTGCATAACAA NAG 2 0.1373 Tier II
14 NC_000076.6 10 63962446 - Lrrtm3 NNNGACTTTCTGCAAGACAA NGG 2 0.1113 Tier II
15 NC_000076.6 10 63962446 - Ctnna3 NNNGACTTTCTGCAAGACAA NGG 2 0.1113 Tier II
16 NC_000075.6 9 53311074 - Exph5 NNNGACTTTCTGGATAACAT NGG 2 0.0818 Tier II
17 NC_000068.7 2 67590592 + B3galt1 NNNGAGTTTCTGCATAACAA NGA 2 0.0347 Tier II
18 NC_000073.6 7 51964883 - Gas2 NNNGACTTTCTGCATAAGCA NGG 2 0.0276 Tier II
19 NC_000073.6 7 90889863 - Dlg2 NNNGACTTTCTGCATAAGAA NGT 2 0.0022 Tier II
20 NC_000073.6 7 76929512 + Agbl1 NNNGACTTTCTGCAGCACAA NGG 2 0.0 Tier II
21 NC_000074.6 8 22061410 + Alg11 NNNGACTTTCTGCATTATAA NGG 2 0.0 Tier II
22 NC_000086.7 X 43207259 + Tenm1 NNNTACTTTCTGCATTACAA NGG 2 0.0 Tier II
23 NC_000067.6 1 156751589 + Gm39683 NNNGACTTTCTGGGTAACAA NGG 2 0.0636 Tier III
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Other clones with same target sequence:

(none)