Construct: sgRNA BRDN0001148172
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTAAATAGTTACCTCCTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KSR2 (283455)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76426
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
117558511 |
+ |
KSR2 |
NNNAATAGTTACCTCCTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
10306054 |
- |
ZGLP1 |
NNNATTAGTTGCCTCCTCGG |
NGG |
2 |
0.2364 |
Tier II |
| 3 |
NC_000003.12 |
3 |
186805709 |
- |
RFC4 |
NNNAATAGTTACCTTCTGGG |
NGG |
2 |
0.0089 |
Tier II |
| 4 |
NC_000019.10 |
19 |
10306054 |
- |
FDX2-ZGLP1 |
NNNATTAGTTGCCTCCTCGG |
NGG |
2 |
0.2364 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
117685551 |
- |
Ksr2 |
NNNAATACTTACCTCCTCGG |
NGG |
1 |
0.6154 |
Tier I |
| 2 |
NC_000071.6 |
5 |
73218558 |
+ |
Fryl |
NNNAATAATTACCTCCTCTG |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000082.6 |
16 |
7074892 |
- |
Rbfox1 |
NNNAATAGTTACTTCCTCTG |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000073.6 |
7 |
79314049 |
+ |
Abhd2 |
NNNAATATTTACCTCCTCTG |
NGG |
2 |
0.4167 |
Tier II |
| 5 |
NC_000079.6 |
13 |
24643139 |
- |
Ripor2 |
NNNAATAGTTACCTGCTAGG |
NGG |
2 |
0.0269 |
Tier II |
| 6 |
NC_000075.6 |
9 |
86670993 |
- |
Me1 |
NNNAATAGTTCCCTCCTCGG |
NTG |
2 |
0.0156 |
Tier II |
| 7 |
NC_000076.6 |
10 |
111339723 |
+ |
Gm40761 |
NNNAATATTTACCTCCTCTG |
NGG |
2 |
0.4167 |
Tier III |
Other clones with same target sequence:
(none)
