Construct: sgRNA BRDN0001148183

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAGGGTGCACATCAAAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP4K1 (11184)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77608

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 38612631 + MAP4K1 NNNGGGTGCACATCAAAGAG NGG 0 1.0 Tier I
2 NC_000017.11 17 7836228 - LOC124904106 NNNTGGTGCACATCAAAGAG NAG 2 0.0943 Tier I
3 NC_000009.12 9 95231748 + FANCC NNNGGGTGCAAATCAAAGAG NGG 1 0.75 Tier II
4 NC_000012.12 12 111867737 - MAPKAPK5 NNNGGGAGCACATAAAAGAG NGG 2 0.3062 Tier II
5 NC_000006.12 6 41582546 - FOXP4 NNNGGGTGCCCAACAAAGAG NGG 2 0.2308 Tier II
6 NC_000012.12 12 2835438 + ITFG2 NNNGGGTTCAGATCAAAGAG NGG 2 0.1563 Tier II
7 NC_000020.11 20 32538333 + NOL4L NNNGGGTGCACACCAAGGAG NGG 2 0.1393 Tier II
8 NC_000006.12 6 11242692 - NEDD9 NNNGGGGGCACATCAGAGAG NGG 2 0.1131 Tier II
9 NC_000017.11 17 7836228 - KDM6B NNNTGGTGCACATCAAAGAG NAG 2 0.0943 Tier II
10 NC_000020.11 20 19448977 - SLC24A3 NNNGGGTGCACATCCAGGAG NGG 2 0.0481 Tier II
11 NC_000012.12 12 121808359 - SETD1B NNNGGGGGCACATCAAAGAG NTG 2 0.0229 Tier II
12 NC_000002.12 2 241038935 - SNED1 NNNGGCTGCACATCACAGAG NGG 2 0.0 Tier II
13 NC_000013.11 13 114106080 + RASA3 NNNGGGTGCACATCGCAGAG NGG 2 0.0 Tier II
14 NC_000019.10 19 56706653 + LOC105372472 NNNGGGAGAACATCAAAGAG NGG 2 0.75 Tier III
15 NC_000019.10 19 56706653 + LOC105372473 NNNGGGAGAACATCAAAGAG NGG 2 0.75 Tier III
16 NC_000003.12 3 44478025 + LOC101928529 NNNGGGTACACTTCAAAGAG NGG 2 0.3333 Tier III
17 NC_000006.12 6 128694015 + LOC105377998 NNNGGGTGCACATTAAAGAG NAG 2 0.1901 Tier III
18 NC_000005.10 5 6763542 + LOC107986401 NNNGGGTGCAGAACAAAGAG NGG 2 0.1731 Tier III
19 NC_000020.11 20 32538333 + LOC105372592 NNNGGGTGCACACCAAGGAG NGG 2 0.1393 Tier III
20 NC_000002.12 2 241038935 - SNED1-AS1 NNNGGCTGCACATCACAGAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 28988186 - Map4k1 NNNGGGTGCACATCAAAGAG NGG 0 1.0 Tier I
2 NC_000067.6 1 131061880 + Mapkapk2 NNNGGGTTAACATCAAAGAG NGG 2 0.5357 Tier II
3 NC_000074.6 8 48432322 - Tenm3 NNNGGGTTAACATCAAAGAG NGG 2 0.5357 Tier II
4 NC_000076.6 10 40672979 - Ddo NNNGGGTGGACAACAAAGAG NGG 2 0.4286 Tier II
5 NC_000071.6 5 103089779 + Mapk10 NNNGAGTGCACATCAAAGAG NAG 2 0.2247 Tier II
6 NC_000077.6 11 110520794 + Map2k6 NNNGGATGCACATCTAAGAG NGG 2 0.2 Tier II
7 NC_000072.6 6 135934733 + Grin2b NNNGGTTGCACATCAATGAG NGG 2 0.0889 Tier II
8 NC_000067.6 1 66356873 + Map2 NNNGTGTGCACATCAAAGAG NAG 2 0.0778 Tier II
9 NC_000072.6 6 61550701 + Ccser1 NNNGGGTGCACATCCAAGAG NAG 2 0.0707 Tier II
10 NC_000070.6 4 139394230 + Ubr4 NNNGGGTGCAGATCAAGGAG NGG 2 0.0441 Tier II
11 NC_000067.6 1 58188889 - Aox3 NNNGGGTGCAGATCAAAGAG NTG 2 0.0097 Tier II
12 NC_000075.6 9 116628813 - Rbms3 NNNGGGTGAACATGAAAGAG NGG 2 0.0 Tier II
13 NC_000080.6 14 17406996 + Gm32121 NNNTGGTGCTCATCAAAGAG NGG 2 0.3209 Tier III
14 NC_000073.6 7 81735135 - Gm42400 NNNGTGTGCCCATCAAAGAG NGG 2 0.1 Tier III
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Other clones with same target sequence:

(none)