Construct: sgRNA BRDN0001148198
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGAAGAAGTTGGGCCATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP5K1C (23396)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77665
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
3664862 |
- |
PIP5K1C |
NNNAAGAAGTTGGGCCATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
160141812 |
+ |
SLC22A1 |
NNNAAGAAGGAGGGCCATCG |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000017.11 |
17 |
79150547 |
- |
RBFOX3 |
NNNAAGAAGTTGAGCCAGCG |
NGG |
2 |
0.3077 |
Tier II |
| 4 |
NC_000001.11 |
1 |
155950755 |
- |
ARHGEF2 |
NNNAAGAAATTGGGCCATTG |
NGG |
2 |
0.2967 |
Tier II |
| 5 |
NC_000014.9 |
14 |
70784387 |
- |
MAP3K9 |
NNNCAGAAGTTGGGCCATCG |
NAG |
2 |
0.0915 |
Tier II |
| 6 |
NC_000008.11 |
8 |
51647350 |
+ |
PXDNL |
NNNAAGAAGTTGGGCCAAGG |
NGG |
2 |
0.0833 |
Tier II |
| 7 |
NC_000005.10 |
5 |
173295481 |
+ |
LOC105377731 |
NNNAAGAAGTAGGGCCATGG |
NGG |
2 |
0.0938 |
Tier III |
| 8 |
NC_000020.11 |
20 |
10885225 |
+ |
LOC101929413 |
NNNAAGAAGTTGGGCCATCT |
NGT |
2 |
0.0113 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
81305239 |
+ |
Pip5k1c |
NNNAAGAAGCTGGGCCACCG |
NGG |
2 |
0.2286 |
Tier I |
| 2 |
NC_000076.6 |
10 |
68210482 |
- |
Arid5b |
NNNGAGAAGTTGGGCCATTG |
NGG |
2 |
0.2885 |
Tier II |
| 3 |
NC_000080.6 |
14 |
118566138 |
+ |
Abcc4 |
NNNCAGAAGTTGGGCCATCT |
NGG |
2 |
0.2471 |
Tier II |
| 4 |
NC_000084.6 |
18 |
73858427 |
- |
Mro |
NNNAAGATGTTGGGCCATGG |
NGG |
2 |
0.0536 |
Tier II |
| 5 |
NC_000085.6 |
19 |
3718762 |
- |
Gm36787 |
NNNAAGAATTTGGGCCATCA |
NGG |
2 |
0.5 |
Tier III |
| 6 |
NC_000076.6 |
10 |
68210482 |
- |
Gm51770 |
NNNGAGAAGTTGGGCCATTG |
NGG |
2 |
0.2885 |
Tier III |
| 7 |
NC_000079.6 |
13 |
107137017 |
- |
Gm31452 |
NNNAAGCAGTTGGGCCACCG |
NGG |
2 |
0.1875 |
Tier III |
| 8 |
NC_000084.6 |
18 |
73858427 |
- |
Gm32661 |
NNNAAGATGTTGGGCCATGG |
NGG |
2 |
0.0536 |
Tier III |
Other clones with same target sequence:
(none)
