Construct: sgRNA BRDN0001148200
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATGCTAACAGACTTACAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DLG1 (1739)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76617
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000003.12 |
3 |
197130527 |
+ |
DLG1 |
NNNGCTAACAGACTTACAGT |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000022.11 |
22 |
35984588 |
+ |
RBFOX2 |
NNNGCTAAAAGACTTACAGA |
NGG |
2 |
0.4821 |
Tier II |
3 |
NC_000023.11 |
X |
74851522 |
+ |
NEXMIF |
NNNCCTAACAGACTAACAGT |
NGG |
2 |
0.3065 |
Tier II |
4 |
NC_000017.11 |
17 |
33509517 |
+ |
ASIC2 |
NNNGCTAACAGACTGACAGA |
NGG |
2 |
0.0281 |
Tier II |
5 |
NC_000023.11 |
X |
74487445 |
+ |
SLC16A2 |
NNNGCAAACAGACTTACAGT |
NGC |
2 |
0.0193 |
Tier II |
6 |
NC_000013.11 |
13 |
66884633 |
+ |
PCDH9 |
NNNGCTAACATACTGACAGT |
NGG |
2 |
0.0192 |
Tier II |
7 |
NC_000001.11 |
1 |
60032915 |
+ |
C1orf87 |
NNNGCTAACAGAGTTACAGT |
NGT |
2 |
0.0022 |
Tier II |
8 |
NC_000021.9 |
21 |
31190022 |
- |
TIAM1 |
NNNGCTAACAGCCTTCCAGT |
NGG |
2 |
0.0 |
Tier II |
9 |
NC_000013.11 |
13 |
66884633 |
+ |
PCDH9-AS2 |
NNNGCTAACATACTGACAGT |
NGG |
2 |
0.0192 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000081.6 |
15 |
97987293 |
+ |
Col2a1 |
NNNGCTAATAGACTTACAGA |
NGG |
2 |
0.4922 |
Tier I |
2 |
NC_000076.6 |
10 |
69867762 |
- |
Ank3 |
NNNGCTAACAGACTTACAGA |
NGG |
1 |
0.5625 |
Tier II |
3 |
NC_000082.6 |
16 |
94895674 |
+ |
Kcnj6 |
NNNGATAACAGACTTACAGA |
NGG |
2 |
0.3214 |
Tier II |
4 |
NC_000080.6 |
14 |
9604801 |
+ |
Fhit |
NNNGCTGACAGACTTACACT |
NGG |
2 |
0.3164 |
Tier II |
5 |
NC_000070.6 |
4 |
141959862 |
+ |
Fhad1 |
NNNTGTAACAGACTTACAGT |
NGG |
2 |
0.2182 |
Tier II |
6 |
NC_000067.6 |
1 |
39952490 |
+ |
Map4k4 |
NNNGCTCACAGACTTATAGT |
NGG |
2 |
0.2042 |
Tier II |
7 |
NC_000075.6 |
9 |
10132149 |
- |
Cntn5 |
NNNGCTAACACACTCACAGT |
NGG |
2 |
0.1169 |
Tier II |
8 |
NC_000077.6 |
11 |
21892551 |
- |
Wdpcp |
NNNGCTAACAGACTCACAGT |
NAG |
2 |
0.0707 |
Tier II |
9 |
NC_000068.7 |
2 |
41411234 |
+ |
Lrp1b |
NNNGCTAACAGAATTACAGG |
NGG |
2 |
0.0679 |
Tier II |
10 |
NC_000070.6 |
4 |
3998552 |
+ |
Sdr16c5 |
NNNGCTAACAGAATTACAGG |
NGG |
2 |
0.0679 |
Tier II |
11 |
NC_000068.7 |
2 |
158521041 |
+ |
Arhgap40 |
NNNGCTCACAGAGTTACAGT |
NGG |
2 |
0.0597 |
Tier II |
12 |
NC_000067.6 |
1 |
72357042 |
+ |
Xrcc5 |
NNNGCTTACAGAGTTACAGT |
NGG |
2 |
0.0597 |
Tier II |
13 |
NC_000068.7 |
2 |
14946302 |
+ |
Cacnb2 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier II |
14 |
NC_000074.6 |
8 |
69059832 |
- |
Slc18a1 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier II |
15 |
NC_000083.6 |
17 |
68407380 |
+ |
L3mbtl4 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier II |
16 |
NC_000083.6 |
17 |
68622108 |
- |
L3mbtl4 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier II |
17 |
NC_000086.7 |
X |
83251239 |
- |
Dmd |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier II |
18 |
NC_000071.6 |
5 |
27090067 |
+ |
Dpp6 |
NNNGCTAACAGACTGGCAGT |
NGG |
2 |
0.0096 |
Tier II |
19 |
NC_000074.6 |
8 |
68902847 |
- |
Lpl |
NNNGCTAACAGACTTGCAGT |
NGT |
2 |
0.0031 |
Tier II |
20 |
NC_000070.6 |
4 |
108050092 |
+ |
Scp2 |
NNNGCTAACAGACTGAGAGT |
NGG |
2 |
0.0029 |
Tier II |
21 |
NC_000073.6 |
7 |
97010692 |
+ |
Nars2 |
NNNGCTAACAGACTTACAGC |
NGT |
2 |
0.0015 |
Tier II |
22 |
NC_000077.6 |
11 |
57516581 |
- |
Fam114a2 |
NNNGCTAACAGACTTCCAGT |
NTG |
2 |
0.0 |
Tier II |
23 |
NC_000072.6 |
6 |
90168363 |
+ |
Gm20426 |
NNNGCTAACAGACTCACAGA |
NGG |
2 |
0.1534 |
Tier III |
24 |
NC_000071.6 |
5 |
8784296 |
+ |
Gm5106 |
NNNGCTAACAGAATCACAGT |
NGG |
2 |
0.1049 |
Tier III |
25 |
NC_000074.6 |
8 |
52772318 |
- |
Gm9685 |
NNNGCTAACAGAATCACAGT |
NGG |
2 |
0.1049 |
Tier III |
26 |
NC_000072.6 |
6 |
69321395 |
- |
Igk |
NNNGCTTACAGACTTACAGG |
NGG |
2 |
0.0772 |
Tier III |
27 |
NC_000073.6 |
7 |
17276952 |
- |
Gm42373 |
NNNGCTAACAGAATTACAGG |
NGG |
2 |
0.0679 |
Tier III |
28 |
NC_000073.6 |
7 |
75069882 |
- |
4930533N22Rik |
NNNGCTAACAGAATTACAGG |
NGG |
2 |
0.0679 |
Tier III |
29 |
NC_000068.7 |
2 |
78822475 |
- |
Gm39865 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier III |
30 |
NC_000070.6 |
4 |
118945125 |
+ |
Gm12865 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier III |
31 |
NC_000072.6 |
6 |
60123154 |
- |
Gm5570 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier III |
32 |
NC_000072.6 |
6 |
141882803 |
+ |
Gm30843 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier III |
33 |
NC_000074.6 |
8 |
39900051 |
+ |
Gm36795 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier III |
34 |
NC_000075.6 |
9 |
51051166 |
- |
Gm32681 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier III |
35 |
NC_000078.6 |
12 |
34960969 |
- |
Gm33864 |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier III |
36 |
NC_000082.6 |
16 |
98063947 |
- |
A630089N07Rik |
NNNGCTAACAGACTCACAGG |
NGG |
2 |
0.0481 |
Tier III |
Other clones with same target sequence:
(none)