Construct: sgRNA BRDN0001148210
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGAGCGAGCGGTGCATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RAB11FIP5 (26056)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76185
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
73112441 |
+ |
RAB11FIP5 |
NNNGAGCGAGCGGTGCATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
143726520 |
- |
FAM83H |
NNNGGGCGAGCGGCGCATGG |
NGG |
2 |
0.2057 |
Tier I |
| 3 |
NC_000019.10 |
19 |
1568058 |
+ |
MEX3D |
NNNGAGCGAGCTGGGCATGG |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000001.11 |
1 |
17599397 |
+ |
ARHGEF10L |
NNNGAGCAAGCAGTGCATGG |
NGG |
2 |
0.9333 |
Tier II |
| 5 |
NC_000004.12 |
4 |
142290532 |
- |
INPP4B |
NNNGAGTGAGCAGTGCATGG |
NGG |
2 |
0.7583 |
Tier II |
| 6 |
NC_000009.12 |
9 |
20776304 |
+ |
FOCAD |
NNNGAGCAAGAGGTGCATGG |
NGG |
2 |
0.75 |
Tier II |
| 7 |
NC_000018.10 |
18 |
25282569 |
+ |
ZNF521 |
NNNGAGGGGGCGGTGCATGG |
NGG |
2 |
0.3137 |
Tier II |
| 8 |
NC_000010.11 |
10 |
66559632 |
- |
CTNNA3 |
NNNGAGCGGGGGGTGCATGG |
NGG |
2 |
0.1667 |
Tier II |
| 9 |
NC_000014.9 |
14 |
58468342 |
- |
KIAA0586 |
NNNGAGCCAGCGGGGCATGG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
85374192 |
+ |
Rab11fip5 |
NNNGAGTGAGCGATGCATGG |
NGG |
2 |
0.75 |
Tier I |
| 2 |
NC_000068.7 |
2 |
155629631 |
+ |
Myh7b |
NNNGAGCGAGCTGCGCATGG |
NGG |
2 |
0.1099 |
Tier I |
| 3 |
NC_000068.7 |
2 |
166150652 |
- |
Sulf2 |
NNNGAGCAAGCGGTGAATGG |
NGG |
2 |
1.0 |
Tier II |
| 4 |
NC_000069.6 |
3 |
89238425 |
+ |
Trim46 |
NNNGAGCGAGCGGTGCATGA |
NGG |
1 |
0.9375 |
Tier II |
| 5 |
NC_000077.6 |
11 |
4109348 |
+ |
Sec14l2 |
NNNGAGCAAGCAGTGCATGG |
NGG |
2 |
0.9333 |
Tier II |
| 6 |
NC_000070.6 |
4 |
9235211 |
- |
Clvs1 |
NNNGAGTGAGCAGTGCATGG |
NGG |
2 |
0.7583 |
Tier II |
| 7 |
NC_000073.6 |
7 |
144663546 |
- |
Ano1 |
NNNGAGTGAGCAGTGCATGG |
NGG |
2 |
0.7583 |
Tier II |
| 8 |
NC_000069.6 |
3 |
69505224 |
+ |
Ppm1l |
NNNCAGCGAGCCGTGCATGG |
NGG |
2 |
0.2803 |
Tier II |
| 9 |
NC_000067.6 |
1 |
160585283 |
- |
Rabgap1l |
NNNGAGTGAGTGGTGCATGG |
NGG |
2 |
0.25 |
Tier II |
| 10 |
NC_000082.6 |
16 |
6348545 |
- |
Rbfox1 |
NNNGAGCGCGCGGTGCAGGG |
NGG |
2 |
0.1905 |
Tier II |
| 11 |
NC_000071.6 |
5 |
117327239 |
- |
Vsig10 |
NNNGAGCGTGGGGTGCATGG |
NGG |
2 |
0.15 |
Tier II |
| 12 |
NC_000079.6 |
13 |
48803418 |
- |
Phf2 |
NNNGAGCGAGCGGAGCATGG |
NCG |
2 |
0.0663 |
Tier II |
| 13 |
NC_000081.6 |
15 |
98868865 |
- |
Kmt2d |
NNNGAGCGAGCTGTGCTTGG |
NGG |
2 |
0.0513 |
Tier II |
| 14 |
NC_000071.6 |
5 |
149202717 |
+ |
Uspl1 |
NNNGAGCGAGCGGTGCATAG |
NGA |
2 |
0.0496 |
Tier II |
| 15 |
NC_000086.7 |
X |
162869595 |
- |
Syap1 |
NNNGAGAGAGCGGGGCATGG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000075.6 |
9 |
11277719 |
+ |
Gm18934 |
NNNGAACGAGCGATGCATGG |
NGG |
2 |
0.9231 |
Tier III |
| 17 |
NC_000079.6 |
13 |
71103900 |
+ |
Gm40997 |
NNNGAGAGAGCGGTTCATGG |
NGG |
2 |
0.1071 |
Tier III |
Other clones with same target sequence:
(none)
