Construct: sgRNA BRDN0001148228
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTCATATCACCTTACACTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAPSS1 (9061)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77613
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
107693776 |
- |
PAPSS1 |
NNNCATATCACCTTACACTC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
44755134 |
- |
TRIM69 |
NNNCATAGCATCTTACACTC |
NGG |
2 |
0.2256 |
Tier I |
| 3 |
NC_000014.9 |
14 |
36846010 |
- |
SLC25A21 |
NNNTATATTACCTTACACTC |
NGG |
2 |
0.7 |
Tier II |
| 4 |
NC_000006.12 |
6 |
82221782 |
+ |
IBTK |
NNNCATATCACCTCAAACTC |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000012.12 |
12 |
72649844 |
+ |
TRHDE |
NNNAATATCACCTCACACTC |
NGG |
2 |
0.2406 |
Tier II |
| 6 |
NC_000002.12 |
2 |
3783625 |
+ |
DCDC2C |
NNNCATATCACCTTGCACAC |
NGG |
2 |
0.1857 |
Tier II |
| 7 |
NC_000007.14 |
7 |
128310994 |
+ |
RBM28 |
NNNTATATCACCTTACCCTC |
NGG |
2 |
0.1412 |
Tier II |
| 8 |
NC_000023.11 |
X |
108191580 |
+ |
COL4A6 |
NNNTATATCACCTTACTCTC |
NGG |
2 |
0.1067 |
Tier II |
| 9 |
NC_000002.12 |
2 |
84693336 |
+ |
DNAH6 |
NNNCTTATCACCTTCCACTC |
NGG |
2 |
0.0992 |
Tier II |
| 10 |
NC_000007.14 |
7 |
122459475 |
+ |
CADPS2 |
NNNCTTATCAGCTTACACTC |
NGG |
2 |
0.0909 |
Tier II |
| 11 |
NC_000002.12 |
2 |
216334392 |
- |
MARCHF4 |
NNNCATATCACCTTACTTTC |
NGG |
2 |
0.0857 |
Tier II |
| 12 |
NC_000003.12 |
3 |
68033887 |
+ |
TAFA1 |
NNNCATATCACCTTTCACTT |
NGG |
2 |
0.06 |
Tier II |
| 13 |
NC_000010.11 |
10 |
100214676 |
- |
CHUK |
NNNCATATCAACTTACACTC |
NTG |
2 |
0.0292 |
Tier II |
| 14 |
NC_000003.12 |
3 |
79204740 |
- |
ROBO1 |
NNNCATATCACTTTACACTC |
NTG |
2 |
0.021 |
Tier II |
| 15 |
NC_000006.12 |
6 |
77269505 |
- |
LOC101928570 |
NNNCAAAACACCTTACACTC |
NGG |
2 |
0.6933 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
54526323 |
- |
Pgap1 |
NNNCACATCACTTTACACTC |
NGG |
2 |
0.4895 |
Tier II |
| 2 |
NC_000079.6 |
13 |
73263574 |
+ |
Irx4 |
NNNCAGAGCACCTTACACTC |
NGG |
2 |
0.419 |
Tier II |
| 3 |
NC_000075.6 |
9 |
117348910 |
- |
Rbms3 |
NNNCAGATCACATTACACTC |
NGG |
2 |
0.4082 |
Tier II |
| 4 |
NC_000078.6 |
12 |
4594509 |
- |
Itsn2 |
NNNCATATCTCCTCACACTC |
NGG |
2 |
0.2521 |
Tier II |
| 5 |
NC_000069.6 |
3 |
86846707 |
+ |
Dclk2 |
NNNCACATCAGCTTACACTC |
NGG |
2 |
0.2273 |
Tier II |
| 6 |
NC_000069.6 |
3 |
29302405 |
+ |
Egfem1 |
NNNCCTTTCACCTTACACTC |
NGG |
2 |
0.2188 |
Tier II |
| 7 |
NC_000071.6 |
5 |
105237988 |
- |
Gbp10 |
NNNCATTTCACCTTTCACTC |
NGG |
2 |
0.0875 |
Tier II |
| 8 |
NC_000078.6 |
12 |
85614925 |
- |
Jdp2 |
NNNCATATCAGCTTAGACTC |
NGG |
2 |
0.0385 |
Tier II |
| 9 |
NC_000077.6 |
11 |
94582522 |
+ |
Acsf2 |
NNNCATGTCACCTGACACTC |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000067.6 |
1 |
54526323 |
- |
Gm51623 |
NNNCACATCACTTTACACTC |
NGG |
2 |
0.4895 |
Tier III |
| 11 |
NC_000075.6 |
9 |
117348910 |
- |
Gm39451 |
NNNCAGATCACATTACACTC |
NGG |
2 |
0.4082 |
Tier III |
Other clones with same target sequence:
(none)
