Construct: sgRNA BRDN0001148241
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTACCCCTATTATAATGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80235
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
20932782 |
+ |
EIF4G3 |
NNNACACCTATAATAATGAA |
NGG |
2 |
0.7429 |
Tier II |
| 2 |
NC_000009.12 |
9 |
4132695 |
- |
GLIS3 |
NNNACCTCTTTTATAATGAA |
NGG |
2 |
0.7169 |
Tier II |
| 3 |
NC_000021.9 |
21 |
17972190 |
+ |
CHODL |
NNNACCCATATCATAATGAA |
NGG |
2 |
0.6158 |
Tier II |
| 4 |
NC_000004.12 |
4 |
110540774 |
- |
ENPEP |
NNNATCCCCATTATAATGAA |
NGG |
2 |
0.5874 |
Tier II |
| 5 |
NC_000007.14 |
7 |
39991088 |
+ |
CDK13 |
NNNTCCCCTTTTATAATGAA |
NGG |
2 |
0.5615 |
Tier II |
| 6 |
NC_000003.12 |
3 |
98010196 |
+ |
GABRR3 |
NNNACCTATATTATAATGAA |
NGG |
2 |
0.5281 |
Tier II |
| 7 |
NC_000010.11 |
10 |
127452238 |
- |
DOCK1 |
NNNACGCCTATTATAATAAA |
NGG |
2 |
0.3462 |
Tier II |
| 8 |
NC_000021.9 |
21 |
29589082 |
+ |
GRIK1 |
NNNAACCCTGTTATAATGAA |
NGG |
2 |
0.3175 |
Tier II |
| 9 |
NC_000011.10 |
11 |
87836920 |
+ |
RAB38 |
NNNACCCCCATTTTAATGAA |
NGG |
2 |
0.2769 |
Tier II |
| 10 |
NC_000001.11 |
1 |
46574722 |
+ |
MKNK1 |
NNNACTCCTATTATAATGAA |
NAG |
2 |
0.2407 |
Tier II |
| 11 |
NC_000001.11 |
1 |
231718005 |
+ |
DISC1 |
NNNTCCCCTATTACAATGAA |
NGG |
2 |
0.1818 |
Tier II |
| 12 |
NC_000003.12 |
3 |
124455859 |
- |
KALRN |
NNNACCCCTATTATAAAGAA |
NAG |
2 |
0.1383 |
Tier II |
| 13 |
NC_000007.14 |
7 |
84353379 |
- |
SEMA3A |
NNNACCCCTATTATAATAAA |
NGA |
2 |
0.0481 |
Tier II |
| 14 |
NC_000018.10 |
18 |
48106453 |
+ |
ZBTB7C |
NNNAACCCTATTATAATGAA |
NGA |
2 |
0.0397 |
Tier II |
| 15 |
NC_000002.12 |
2 |
141248373 |
- |
LRP1B |
NNNACCCCTATTATAAGGAA |
NTG |
2 |
0.0046 |
Tier II |
| 16 |
NC_000007.14 |
7 |
28808409 |
+ |
CREB5 |
NNNACCACTATTAGAATGAA |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000007.14 |
7 |
78615222 |
+ |
MAGI2 |
NNNACCCCTATTAAATTGAA |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000009.12 |
9 |
24053414 |
- |
LOC124902327 |
NNNACCACTTTTATAATGAA |
NGG |
2 |
0.6618 |
Tier III |
| 19 |
NC_000011.10 |
11 |
87836920 |
+ |
LOC107984361 |
NNNACCCCCATTTTAATGAA |
NGG |
2 |
0.2769 |
Tier III |
| 20 |
NC_000001.11 |
1 |
231718005 |
+ |
TSNAX-DISC1 |
NNNTCCCCTATTACAATGAA |
NGG |
2 |
0.1818 |
Tier III |
| 21 |
NC_000006.12 |
6 |
18584404 |
- |
MIR548A1HG |
NNNACCCCTATTTTAGTGAA |
NGG |
2 |
0.0577 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
43232424 |
+ |
Hpse2 |
NNNACCCTTATAATAATGAA |
NGG |
2 |
0.7 |
Tier II |
| 2 |
NC_000073.6 |
7 |
87605305 |
+ |
Grm5 |
NNNACACCTATTAAAATGAA |
NGG |
2 |
0.5748 |
Tier II |
| 3 |
NC_000085.6 |
19 |
46360498 |
- |
Mfsd13a |
NNNACCCATATAATAATGAA |
NGG |
2 |
0.52 |
Tier II |
| 4 |
NC_000080.6 |
14 |
51888946 |
- |
Mettl17 |
NNNTCCCCTATAATAATGAA |
NGG |
2 |
0.5091 |
Tier II |
| 5 |
NC_000073.6 |
7 |
75654273 |
+ |
Akap13 |
NNNACCTCTGTTATAATGAA |
NGG |
2 |
0.4514 |
Tier II |
| 6 |
NC_000078.6 |
12 |
81225810 |
+ |
Slc8a3 |
NNNTCCCCTATTATAACGAA |
NGG |
2 |
0.4492 |
Tier II |
| 7 |
NC_000068.7 |
2 |
27119545 |
- |
Fam163b |
NNNACCCCTGTTATAATCAA |
NGG |
2 |
0.2646 |
Tier II |
| 8 |
NC_000079.6 |
13 |
81140662 |
- |
Adgrv1 |
NNNCCCCCTATTATAATGAA |
NTG |
2 |
0.0138 |
Tier II |
| 9 |
NC_000067.6 |
1 |
118525933 |
- |
Clasp1 |
NNNACCCCTATTATAAAGAA |
NGC |
2 |
0.0119 |
Tier II |
| 10 |
NC_000082.6 |
16 |
3762175 |
- |
Gm36195 |
NNNACCCCTCTCATAATGAA |
NGG |
2 |
0.3158 |
Tier III |
| 11 |
NC_000067.6 |
1 |
187489420 |
+ |
Gm36249 |
NNNACCCCTATAATAAGGAA |
NGG |
2 |
0.0941 |
Tier III |
| 12 |
NC_000072.6 |
6 |
67679188 |
+ |
Igk |
NNNACCCCTATTATGATGAA |
NGA |
2 |
0.0451 |
Tier III |
Other clones with same target sequence:
(none)
